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anti-Human TGFA antibody for Functional Studies

Recommended TGFA Antibody (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) Antibodies
  • LOC100009482
  • NV15606
  • RATTGFAA
  • TFGA
  • TGFA
  • TGFAA
  • TGF alpha
  • wa-1
  • wa1
Reactivity
Human
333
124
121
87
60
32
4
2
1
1
Host
Rabbit
201
134
21
4
1
Clonality
Polyclonal
Conjugate
This TGFA antibody is un-conjugated
27
13
10
8
8
8
7
7
7
7
7
7
7
7
7
7
7
7
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
201
110
101
95
77
67
65
58
55
17
11
7
4
3
3
2
2
1
1
Supplier
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Catalog No. ABIN116239
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008771965 ABIN116240 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal 4
0.0008771965 ABIN2476746 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0008771965 ABIN641065 Func ELISA WB Rabbit IgG Log in to see

Top referenced anti-TGFA antibody for Functional Studies

Similar anti-TGFA Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 92 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 93 Antibodies
Functional Studies (Func) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 53 Antibodies
Immunofluorescence (IF) 76 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 63 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 52 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 95 Antibodies
Immunoprecipitation (IP) 64 Antibodies
Neutralization (Neut) 17 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies

General

Antigen Transforming Growth Factor, alpha (TGFA) Antibodies
Reactivity Human
(333), (124), (121), (87), (60), (32), (4), (2), (1), (1)
Host Rabbit
(201), (134), (21), (4), (1)
Clonality
Polyclonal
Conjugate This TGFA antibody is un-conjugated
(27), (13), (10), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(201), (110), (101), (95), (77), (67), (65), (58), (55), (17), (11), (7), (4), (3), (3), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-TGFA Antibody

Target Details TGFA Application Details Handling References for anti-TGFA Antibody (ABIN116239) Images
Specificity This antibody reacts with Transforming Growth Factor alpha.
Purification Affinity chromatography.
Immunogen Highly pure (>98%) recombinant hTGF-alpha

Target Details TGFA

Product Details anti-TGFA Antibody Application Details Handling References for anti-TGFA Antibody (ABIN116239) Images back to top
Antigen
Alternative Name TGF-alpha (TGFA) (TGFA Antibody Abstract)
Background Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha
Gene ID 7039
NCBI Accession NP_001093161
UniProt P01135
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-TGFA Antibody Target Details TGFA Handling References for anti-TGFA Antibody (ABIN116239) Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTGF-α (0.5 ng/mL), a concentration of 2.5-2.7 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTGF-α by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTGF-α. (Sandwich): To detect hTGF-α by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TGF-α (PP1070B1 or PP1070B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTGF-α. Western Blot: To detect hTGF-α by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTGF-α is 1.5- 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-TGFA Antibody Target Details TGFA Application Details References for anti-TGFA Antibody (ABIN116239) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-TGFA Antibody (ABIN116239)

Product Details anti-TGFA Antibody Target Details TGFA Application Details Handling Images back to top
Background publications

Li, Fan, Chen, Li, Woodley: "Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration." in: The Journal of investigative dermatology, Vol. 126, Issue 9, pp. 2096-105, 2006

Hasson, Gallula, Shimoni, Grad-Itach, Marikovsky, Mitrani: "Skin-derived micro-organs induce angiogenesis in rabbits." in: Journal of vascular research, Vol. 43, Issue 2, pp. 139-48, 2006

Kramer, Yang, Snodgrass, Li, Scammell, Davis, Weitz: "Regulation of daily locomotor activity and sleep by hypothalamic EGF receptor signaling." in: Science (New York, N.Y.), Vol. 294, Issue 5551, pp. 2511-5, 2001

Xian, Mardell, Read: "Specificity of the localization of transforming growth factor-alpha immunoreactivity in colon mucosa." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 47, Issue 7, pp. 949-58, 1999