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|Antigen||Transforming Growth Factor, alpha (TGFA) Antibodies|
|Conjugate||This TGFA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
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Product Details anti-TGFA AntibodyTarget Details TGFA Application Details Handling References for anti-TGFA antibody (ABIN116240) Images
|Specificity||This antibody reacts with Transforming Growth Factor alpha.|
|Immunogen||Highly pure (>98%) recombinant hTGF-alpha.|
Target Details TGFAProduct Details anti-TGFA Antibody Application Details Handling References for anti-TGFA antibody (ABIN116240) Images back to top
|Alternative Name||TGF-alpha (TGFA) (TGFA Antibody Abstract)|
|Background||Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling|
|Pathways||NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway|
Application DetailsProduct Details anti-TGFA Antibody Target Details TGFA Handling References for anti-TGFA antibody (ABIN116240) Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTGF-α (0.5 ng/mL), a concentration of 2.5-2.7 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTGF-α by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTGF-α. (Sandwich): To detect hTGF-α by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TGF-α (PP1070B1 or PP1070B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTGF-α. Western Blot: To detect hTGF-α by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTGF-α is 1.5- 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TGFA Antibody Target Details TGFA Application Details References for anti-TGFA antibody (ABIN116240) Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|
References for anti-TGFA antibody (ABIN116240)Product Details anti-TGFA Antibody Target Details TGFA Application Details Handling Images back to top
Li, Fan, Chen, Li, Woodley: "Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration." in: The Journal of investigative dermatology, Vol. 126, Issue 9, pp. 2096-105, 2006
Hasson, Gallula, Shimoni, Grad-Itach, Marikovsky, Mitrani: "Skin-derived micro-organs induce angiogenesis in rabbits." in: Journal of vascular research, Vol. 43, Issue 2, pp. 139-48, 2006
Kramer, Yang, Snodgrass, Li, Scammell, Davis, Weitz: "Regulation of daily locomotor activity and sleep by hypothalamic EGF receptor signaling." in: Science (New York, N.Y.), Vol. 294, Issue 5551, pp. 2511-5, 2001
Xian, Mardell, Read: "Specificity of the localization of transforming growth factor-alpha immunoreactivity in colon mucosa." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 47, Issue 7, pp. 949-58, 1999