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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||186 Antibodies||40 Antibodies||53 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Neutralization (Neut)||17 Antibodies|
|Immunoprecipitation (IP)||10 Antibodies||64 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||95 Antibodies||13 Antibodies||6 Antibodies|
|Immunohistochemistry (IHC)||63 Antibodies||19 Antibodies||17 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||52 Antibodies||7 Antibodies||6 Antibodies|
|Antigen||Transforming Growth Factor, alpha (TGFA) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TGFA antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-TGFA AntibodyTarget Details TGFA Application Details Handling Images
|Specificity||This antibody detects endogenous levels of TGF alpha protein. (region surrounding Arg139)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details TGFAProduct Details anti-TGFA Antibody Application Details Handling Images back to top
|Alternative Name||TGF-alpha (TGFA) (TGFA Antibody Abstract)|
|Background||Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33 % homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha|
|Molecular Weight||approx. 10, 17 kDa|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling|
|Pathways||NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway|
Application DetailsProduct Details anti-TGFA Antibody Target Details TGFA Handling Images back to top
ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TGFA Antibody Target Details TGFA Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-TGFA Antibody Target Details TGFA Application Details Handling back to top