anti-Rat (Rattus) TGFA antibody for Immunofluorescence

Recommended TGFA Antibody (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) Antibodies
  • TGFA
  • LOC100009482
  • NV15606
  • RATTGFAA
  • TGFAA
  • wa-1
  • wa1
  • TFGA
  • TGF alpha
  • transforming growth factor, alpha
  • transforming growth factor alpha
  • transforming growth factor-alpha
  • tgfa
  • TGFA
  • LOC100009482
  • LOC100121300
  • Tgfa
  • TGF-A
  • LOC100009150
  • LOC100358936
Epitope
C-Term
101
75
16
6
5
5
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
339
121
117
92
70
36
6
3
2
2
Host
Mouse
204
136
17
4
1
Clonality
Monoclonal
Conjugate
This TGFA antibody is un-conjugated
26
13
10
9
8
8
7
7
7
7
7
7
7
7
7
7
7
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
179
174
164
155
145
116
114
63
62
18
14
13
8
4
4
2
2
2
1
1
Supplier
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Catalog No. ABIN3026161
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2373985 FACS IF IP IHC WB Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358941 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 405 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358942 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 488 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358946 ELISA ICC IF IHC (fro) WB Biotin Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358948 ELISA FACS ICC IF IHC (fro) WB DyLight 680 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358950 ELISA FACS ICC IF IHC (fro) WB DyLight 755 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358879 FACS ICC IF IHC (fro) IP WB Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358943 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 647 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358944 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 700 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358949 ELISA FACS ICC IF IHC (fro) WB DyLight 488 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358947 ELISA FACS ICC IF IHC (fro) WB DyLight 650 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358953 ELISA FACS ICC IF IHC (fro) WB DyLight 350 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358952 ELISA FACS ICC IF IHC (fro) WB DyLight 550 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358877 FACS ICC IF IHC (fro) IP WB Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358878 FACS ICC IF IHC (fro) IP WB Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358954 ELISA FACS ICC IF IHC (fro) WB DyLight 405 Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN2744783 FACS IF IHC (p) WB Mouse IgG1, kappa C-Term Log in to see MF9
1 ABIN4358899 CyTOF ELISA FACS ICC IF IHC (fro) IP WB Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN4358900 CyTOF ELISA FACS ICC IF IHC (fro) IP WB Mouse IgG1 kappa C-Term Log in to see MF9
1 ABIN3026162 FACS IF IHC WB Mouse IgG1, kappa C-Term Log in to see MF9

Similar anti-TGFA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 2 Antibodies 2 Antibodies
ELISA 40 Antibodies 26 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 39 Antibodies 37 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 20 Antibodies 18 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 58 Antibodies

General

Antigen Transforming Growth Factor, alpha (TGFA) Antibodies
Epitope C-Term
(101), (75), (16), (6), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(339), (121), (117), (92), (70), (36), (6), (3), (2), (2)
Host Mouse
(204), (136), (17), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TGFA antibody is un-conjugated
(26), (13), (10), (9), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(179), (174), (164), (155), (145), (116), (114), (63), (62), (18), (14), (13), (8), (4), (4), (2), (2), (2), (1), (1)
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Product Details anti-TGFA Antibody

Target Details TGFA Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
Clone MF9
Isotype IgG1, kappa

Target Details TGFA

Product Details anti-TGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name TGF alpha (TGFA Antibody Abstract)
Background TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
Gene ID 7039
Research Area Cancer, Angiogenesis, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-TGFA Antibody Target Details TGFA Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL, FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

Restrictions For Research Use only

Handling

Product Details anti-TGFA Antibody Target Details TGFA Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-TGFA Antibody Target Details TGFA Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-TGFA antibody (Transforming Growth Factor, alpha) (C-Term) (ABIN3026161) IHC testing of FFPE human testicular carcinoma with TGF alpha antibody