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Mouse (Murine) TNFRSF1A ELISA Kit

Recommended TNFRSF1A Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kits
  • cd120a
  • CD120a
  • fpf
  • FPF
  • MS5
  • p55
  • p55-R
  • p55-r
  • p60
  • TBP1
  • tbp1
  • TNF-alphaR1
  • TNF-R
  • tnf-r
  • TNF-R-I
  • TNF-R1
  • TNF-R55
  • TNFalpha-R1
  • tnfar
  • TNFAR
  • Tnfr-2
  • tnfr1
  • Tnfr1
  • TNFR1
  • TNFR1-d2
  • tnfr55
  • TNFR55
  • TNFR60
  • tnfr60
  • TNFR I
  • TNFRI
  • TNFRp55
  • TNFRSF1A
  • wu:fj65b10
  • xtnfr1
  • zgc:85914
Epitope
AA 30-212
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
27
19
8
7
6
3
2
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2859279
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32748017 ABIN1567756 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32748017 ABIN424877 Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.0012092679 ABIN2762477 Serum, Plasma, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012092679 ABIN456706 0.078-5 ng/mL 0.078 ng/mL Log in to see
0.0012092679 ABIN2109960 Sandwich ELISA Log in to see
0.0012092679 ABIN2952194 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.0012092679 ABIN2952174 0.078-5 ng/mL 0.078 ng/mL Log in to see

Similar TNFRSF1A ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 8 ELISA Kits

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kits
Epitope AA 30-212
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(27), (19), (8), (7), (6), (3), (2), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Supplier Log in to see

Product Details TNFRSF1A ELISA Kit

Target details Application Details Handling Images
Purpose For quantitative detection of mouse TNFR1 in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant mouse TNFR1
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse TNFR1 antibod - 1
  • Lyophilized recombinant mouse TNFR1 standar - 10ng/tubex2
  • Biotinylated anti- mouse TNFR1 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Immunogen Expression system for standard: NSO
Immunogen sequence: L30-A212

Target details

Product Details TNFRSF1A ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name TNFRSF1A (TNFRSF1A ELISA Kit Abstract)
Background Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNF alpha). The protein encoded by this gene is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. And this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate the transcription factor NF-κB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.
Gene ID 21937
UniProt P25118
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details TNFRSF1A ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse TNFR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFR1 has been precoated onto 96-well plates. Standards (NSO, L30-A212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFR1 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TNFR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFR1 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 46, Standard deviation: 2.7, CV(%): 5.9
  • Sample 2: n=16, Mean(pg/ml): 118, Standard deviation: 6.5, CV(%): 5.5
  • Sample 3: n=16, Mean(pg/ml): 581, Standard deviation: 27.5, CV(%): 4.7,
  • Sample 1: n=24, Mean(pg/ml): 48.3, Standard deviation: 2.5, CV(%): 5.2
  • Sample 2: n=24, Mean(pg/ml): 120, Standard deviation: 6, CV(%): 5
  • Sample 3: n=24, Mean(pg/ml): 592, Standard deviation: 33.3, CV(%): 5.6
Restrictions For Research Use only

Handling

Product Details TNFRSF1A ELISA Kit Target details Application Details Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

Product Details TNFRSF1A ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for TNFRSF1A ELISA Kit (Tumor Necrosis Factor Receptor Superfamily, Member 1A) (ABIN2859279) Mouse TNFR1 PicoKine ELISA Kit standard curve