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Human TNFRSF1B ELISA Kit

Recommended TNFRSF1B Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA Kits
  • CD120b
  • LOC100218184
  • MGC83471
  • p75
  • p75TNFR
  • TANK
  • TBPII
  • TNF-alphaR2
  • TNF-R-II
  • TNF-R2
  • TNF-R75
  • TNF-RII/TNFRSF1B
  • TNFalpha-R2
  • TNFBR
  • Tnfr-1
  • TNFR-II
  • TNFR1B
  • Tnfr2
  • TNFR2
  • TNFR80
  • TNFRII
  • TNFRSF1B
  • TRAF2
Epitope
AA 23-257
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
17
12
7
1
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
7.8-500 pg/mL
Minimum Detection Limit
7.8 pg/mL
Application
ELISA
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Catalog No. ABIN411364
$ 350.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32619375 ABIN417657 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.32619375 ABIN1117663 Sandwich ELISA Serum, Plasma, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32619375 ABIN1572806 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.32619375 ABIN2708116 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Urine 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012085857 ABIN367357 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 0.625-40 ng/mL 0.625 ng/mL Log in to see
0.0012085857 ABIN3198618 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012085857 ABIN2685725 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 7.8-500 pg/mL 7.8 pg/mL Log in to see 2
0.0012085857 ABIN2685723 Sandwich ELISA Plasma (EDTA), Plasma (citrate), Plasma (heparin), Cell Culture Supernatant, Serum, Urine 0.16-10.0 ng/mL 0.16 ng/mL Log in to see
0.0012085857 ABIN2685724 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012085857 ABIN2685726 Sandwich ELISA Cell Lysate, Plasma, Serum Log in to see
0.0012085857 ABIN2540118 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012085857 ABIN456708 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012085857 ABIN2109996 Sandwich ELISA Log in to see
0.0012085857 ABIN2116374 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012085857 ABIN2952176 7.813-500 pg/mL 7.813 pg/mL Log in to see
0.0012085857 ABIN3042311 Sandwich ELISA Log in to see

Top referenced TNFRSF1B ELISA Kit for ELISA

Similar TNFRSF1B ELISA Kits

Application / Reactivity Human
ELISA 17 ELISA Kits

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA Kits
Epitope AA 23-257
Reactivity Human
Kits with alternative reactivity to:
(17), (12), (7), (1), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 7.8-500 pg/mL
Minimum Detection Limit 7.8 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details TNFRSF1B ELISA Kit

Target details Application Details Handling References for TNFRSF1B Kit (ABIN411364) Images
Purpose For quantitative detection of human soluble TNFsRII in cell culture supernates, serum, plasma( heparin, EDTA, citrate) and urine.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine
Detection Method Colorimetric
Specificity Natural and recombinant human sTNFsRII
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- human sTNFR II antibod - 1
  • Lyophilized recombinant human sTNFR II standar - 10ng/tubex2
  • Biotinylated anti- human sTNFR II antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: L23-D257

Target details

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Antigen
Alternative Name TNFRSF1B (TNFRSF1B ELISA Kit Abstract)
Background Tumor necrosis factor receptor p75(TNF-R p75, or TNF-R2) is a 75- kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II(TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.
Gene ID 7133
UniProt P20333
Pathways NF-kappaB Signaling, Apoptosis

Application Details

Product Details TNFRSF1B ELISA Kit Target details Handling References for TNFRSF1B Kit (ABIN411364) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human sTNFsRII ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sTNFsRII has been precoated onto 96-well plates. Standards(NSO, L23-D257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sTNFsRII is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sTNFsRII amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human sTNFsRII standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human sTNFsRII standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 63, Standard deviation: 3.09, CV(%): 4.9
  • Sample 2: n=16, Mean(pg/ml): 125, Standard deviation: 7.13, CV(%): 5.7
  • Sample 3: n=16, Mean(pg/ml): 257, Standard deviation: 9.25, CV(%): 3.6,
  • Sample 1: n=24, Mean(pg/ml): 78, Standard deviation: 4.76, CV(%): 6.1
  • Sample 2: n=24, Mean(pg/ml): 134, Standard deviation: 9.65, CV(%): 7.2
  • Sample 3: n=24, Mean(pg/ml): 269, Standard deviation: 14, CV(%): 5.2
Restrictions For Research Use only

Handling

Product Details TNFRSF1B ELISA Kit Target details Application Details References for TNFRSF1B Kit (ABIN411364) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TNFRSF1B Kit (ABIN411364)

Product Details TNFRSF1B ELISA Kit Target details Application Details Handling Images back to top
Background publications

Beltinger, White, Maris, Sulman, Jensen, LePaslier, Stallard, Goeddel, de Sauvage, Brodeur: "Physical mapping and genomic structure of the human TNFR2 gene." in: Genomics, Vol. 35, Issue 1, pp. 94-100, 1996

Images

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Supplier Images
ELISA image for TNFRSF1B ELISA Kit (Tumor Necrosis Factor Receptor Superfamily, Member 1B) (ABIN411364) Human sTNFR II PicoKine ELISA Kit standard curve