TNFRSF1B ELISA Kit
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- Target See all TNFRSF1B ELISA Kits
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
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Reactivity
- Rhesus Monkey
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Custom Rhesus Macaque soluble Tumor Necrosis Factor Receptor 2 (TNFRSF1B) ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificity
- The antibody pair provided in this kit recognizes Rhesus Macaque Tumor Necrosis Factor Receptor 2 (TNFRSF1B)
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Assay Procedure
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all TNFRSF1B ELISA Kits
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
- Alternative Name
- TNFR2 (TNFRSF1B Products)
- Synonyms
- CD120b ELISA Kit, TBPII ELISA Kit, TNF-R-II ELISA Kit, TNF-R75 ELISA Kit, TNFBR ELISA Kit, TNFR1B ELISA Kit, TNFR2 ELISA Kit, TNFR80 ELISA Kit, p75 ELISA Kit, p75TNFR ELISA Kit, TNF-RII/TNFRSF1B ELISA Kit, TNFR-II ELISA Kit, TNFRSF1B ELISA Kit, TNF-R2 ELISA Kit, TNF-alphaR2 ELISA Kit, TNFRII ELISA Kit, TNFalpha-R2 ELISA Kit, Tnfr-1 ELISA Kit, Tnfr2 ELISA Kit, rank ELISA Kit, zgc:163064 ELISA Kit, TNF receptor superfamily member 1B ELISA Kit, tumor necrosis factor receptor superfamily, member 1b ELISA Kit, tumor necrosis factor receptor superfamily, member 1B ELISA Kit, TNFRSF1B ELISA Kit, Tnfrsf1b ELISA Kit, tnfrsf1b ELISA Kit
- Background
- Tumor Necrosis Factor Receptor 2 (TNFRSF1B)
- Gene ID
- 715454
- UniProt
- F7EAE4
- Pathways
- NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
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