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anti-Human TRAF2 antibody for Immunocytochemistry

Recommended TRAF2 Antibody (supplied by: Log in to see )

Antigen
TNF Receptor-Associated Factor 2 (TRAF2) Antibodies
  • AI325259
  • MGC:45012
  • TANK
  • TRAP
  • TRAP3
Epitope
AA 305-325, Middle Region
45
15
13
10
10
9
7
4
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
153
54
43
5
2
1
1
Host
Rabbit
101
39
18
1
Clonality
Polyclonal
Conjugate
This TRAF2 antibody is un-conjugated
6
6
5
4
4
4
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
137
70
55
24
21
20
11
7
5
5
3
3
Supplier
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Catalog No. ABIN3044047
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN252172 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG AA 6-31 Log in to see Polyclonal
0.0010161072 ABIN1875191 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010161072 ABIN2629576 ICC IHC (p) WB Rabbit IgG AA 305-325 Log in to see Polyclonal
0.0010161072 ABIN1821807 ICC WB Rabbit Log in to see Polyclonal
0.0010161072 ABIN2575596 ICC ELISA WB HRP Mouse IgG1, kappa Log in to see 214CT16-3-4
0.0010161072 ABIN2403692 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010161072 ABIN2451845 ICC ELISA WB Biotin Mouse IgG1, kappa Log in to see 214CT16-3-4
0.0010161072 ABIN2988898 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-TRAF2 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 15 Antibodies 70 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 5 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 9 Antibodies
Immunofluorescence (IF) 4 Antibodies 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 33 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen TNF Receptor-Associated Factor 2 (TRAF2) Antibodies
Epitope AA 305-325, Middle Region
(45), (15), (13), (10), (10), (9), (7), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(153), (54), (43), (5), (2), (1), (1)
Host Rabbit
(101), (39), (18), (1)
Clonality
Polyclonal
Conjugate This TRAF2 antibody is un-conjugated
(6), (6), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(137), (70), (55), (24), (21), (20), (11), (7), (5), (5), (3), (3)
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Product Details anti-TRAF2 Antibody

Target Details TRAF2 Application Details Handling Images
Sequence RQHRLDQDKI EALSSKVQQL E
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for TNF receptor-associated factor 2(TRAF2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: TNF receptor-associated factor 2
Protein Name: TNF receptor-associated factor 2
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human TRAF2(305-325aa RQHRLDQDKIEALSSKVQQLE), different from the related rat and mouse sequences by one amino acid.
Isotype IgG

Target Details TRAF2

Product Details anti-TRAF2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TRAF2 (TRAF2 Antibody Abstract)
Background TRAF2(TNF Receptor-Associated Factor 2), also called TRAP, is a protein that in humans is encoded by the TRAF2 gene. The protein encoded by this gene is a member of the TNF receptor(TNFR) associated factor(TRAF) protein family. TRAF2 is a common signal transducer for TNFR2 and CD40 that mediates activation of NF-kappa-B. Rothe et al.(1996) identified ITRAF, which binds to TRAF1, TRAF2, and TRAF3, and that when overexpressed inhibits TRAF2-mediated NF-kappa-B activation. They proposed that ITRAF is an inhibitor of TRAF function that regulates TRAF protein activity by sequestering TRAFs in a latent state in the cytoplasm. Kanamori et al.(2002) found that mouse Traf2 interacted directly with T2bp, and they presented evidence that T2BP is involved in TNF-mediated signaling by its interaction with TRAF2.
UniProt Q12933
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Apoptosis/Necrosis
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-TRAF2 Antibody Target Details TRAF2 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for TRAF2 is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-TRAF2 Antibody Target Details TRAF2 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-TRAF2 Antibody Target Details TRAF2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-TRAF2 antibody (TNF Receptor-Associated Factor 2) (AA 305-325) (ABIN3044047) Anti-TRAF2 antibody, ICC ICC: HELA Cell
Immunohistochemistry (IHC) image for anti-TRAF2 antibody (TNF Receptor-Associated Factor 2) (AA 305-325) (ABIN3044047) Anti-TRAF2 antibody, IHC(P) IHC(P): Human Tonsil Tissue
Western Blotting (WB) image for anti-TRAF2 antibody (TNF Receptor-Associated Factor 2) (AA 305-325) (ABIN3044047) anti-TNF Receptor-Associated Factor 2 (TRAF2) (AA 305-325), (Middle Region) antibody (Image 3)