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anti-Rat (Rattus) TRAF6 antibody for Immunohistochemistry (Frozen Sections)

Recommended TRAF6 Antibody (supplied by: Log in to see )

Antigen
TNF Receptor-Associated Factor 6 (TRAF6) Antibodies
  • 2310003F17Rik
  • AI851288
  • C630032O20Rik
  • CG10961
  • Dmel\\CG10961
  • dtraf2
  • dTraf2
  • DTRAF2
  • dTRAF2
  • dTRAF6
  • fi15f02
  • GB10539
  • MGC:3310
  • RNF85
  • rnf85
  • TRAF
  • Traf2
  • TRAF2
  • traf6
  • TRAF6
  • wu:fi15f02
  • xtraf6
  • zgc:63704
Epitope
AA 145-160, N-Term
22
18
15
11
6
5
5
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
130
58
49
4
4
2
Host
Rabbit
126
10
1
Clonality
Polyclonal
Conjugate
This TRAF6 antibody is un-conjugated
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
116
43
28
26
20
16
16
11
4
4
2
2
1
Supplier
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Available images

Catalog No. ABIN3044469
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN3029156 ICC IHC (fro) IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal

Similar anti-TRAF6 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 12 Antibodies 43 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 11 Antibodies 19 Antibodies
Immunofluorescence (IF) 7 Antibodies 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 39 Antibodies
Immunochromatography (IC) 4 Antibodies

General

Antigen TNF Receptor-Associated Factor 6 (TRAF6) Antibodies
Epitope AA 145-160, N-Term
(22), (18), (15), (11), (6), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(130), (58), (49), (4), (4), (2)
Host Rabbit
(126), (10), (1)
Clonality
Polyclonal
Conjugate This TRAF6 antibody is un-conjugated
(5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(116), (43), (28), (26), (20), (16), (16), (11), (4), (4), (2), (2), (1)
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Product Details anti-TRAF6 Antibody

Target Details TRAF6 Application Details Handling Images
Sequence LRHLEDHQAH CEFALM
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for TNF receptor-associated factor 6(TRAF6) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: TNF receptor-associated factor 6, E3 ubiquitin protein ligase
Protein Name: TNF receptor-associated factor 6
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human TRAF6(145-160aa LRHLEDHQAHCEFALM), different from the related mouse and rat sequences by two amino acids .
Isotype IgG

Target Details TRAF6

Product Details anti-TRAF6 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TRAF6 (TRAF6 Antibody Abstract)
Background TNF receptor-associated factor 6, also called E3 ubiquitin-protein ligase TRAF6 or RNF85, is a TRAF human protein. The protein encoded by this gene is a member of the TNF receptor associated factor(TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This gene is mapped to 11p12. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase(IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein.
UniProt Q9Y4K3
Research Area Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-TRAF6 Antibody Target Details TRAF6 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) , IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-TRAF6 Antibody Target Details TRAF6 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-TRAF6 Antibody Target Details TRAF6 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-TRAF6 antibody (TNF Receptor-Associated Factor 6) (AA 145-160) (ABIN3044469) Lane 9: MCF-7 Cell Lysate
Immunohistochemistry (IHC) image for anti-TRAF6 antibody (TNF Receptor-Associated Factor 6) (AA 145-160) (ABIN3044469) Anti-TRAF6 antibody, IHC(P) IHC(P): Rat Intestine Tissue
Immunohistochemistry (IHC) image for anti-TRAF6 antibody (TNF Receptor-Associated Factor 6) (AA 145-160) (ABIN3044469) Anti-TRAF6 antibody, ICC ICC: HELA Cell
Immunohistochemistry (IHC) image for anti-TRAF6 antibody (TNF Receptor-Associated Factor 6) (AA 145-160) (ABIN3044469) Anti-TRAF6 antibody, IHC(F) IHC(F): Rat Spleen Tissue
Immunohistochemistry (IHC) image for anti-TRAF6 antibody (TNF Receptor-Associated Factor 6) (AA 145-160) (ABIN3044469) Anti-TRAF6 antibody, IHC(F) IHC(F): Rat Liver Tissue
Immunohistochemistry (IHC) image for anti-TRAF6 antibody (TNF Receptor-Associated Factor 6) (AA 145-160) (ABIN3044469) Anti-TRAF6 antibody, IHC(P) IHC(P): Human Tonsil Tissue