anti-Cow (Bovine) CHEK1 antibody for Immunohistochemistry

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
Middle Region
71
66
39
33
16
16
15
15
14
12
6
5
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
485
195
167
14
13
9
9
9
7
4
4
4
3
2
1
1
1
Host
Rabbit
421
55
23
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
411
178
143
76
69
40
25
21
21
10
7
6
6
5
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Catalog No. ABIN2779737
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.897652 ABIN2779738 IHC WB Rabbit Middle Region Log in to see Polyclonal 1
1 ABIN3197992 IHC WB Rabbit pSer296 Log in to see Polyclonal
1 ABIN601007 IHC ELISA WB Rabbit IgG pSer317 Log in to see Polyclonal
1 ABIN601008 IHC WB Rabbit IgG Log in to see Polyclonal

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope Middle Region
(71), (66), (39), (33), (16), (16), (15), (15), (14), (12), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(485), (195), (167), (14), (13), (9), (9), (9), (7), (4), (4), (4), (3), (2), (1), (1), (1)
Host Rabbit
(421), (55), (23), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(411), (178), (143), (76), (69), (40), (25), (21), (21), (10), (7), (6), (6), (5)
Pubmed 1 reference available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN2779737) Images
Blocking Peptide Blocking peptide for this product available: ABIN974903
Sequence SARITIPDIK KDRWYNKPLK KGAKRPRVTS GGVSESPSGF SKHIQSNLDF
Predicted Reactivity Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against CHEK1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CHEK1

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN2779737) Images back to top
Antigen
Alternative Name CHEK1 (CHEK1 Antibody Abstract)
Background CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.
Alias Symbols: CHK1
Protein Interaction Partner: RELA, CUL4A, UBC, SETMAR, DDB1, TNRC6B, ATR, HECW2, CLSPN, DTL, BTG3, TOPBP1, MAPKAPK2, HSP90AA1, CDC25C, RAD51, PIGM, CUL1, VCP, CDC25A, ATM, APP, MED15, NEK2, TLK1, RB1, BRCA1, FEM1B, XIAP, COPS5, MEPE, LATS2, TP53, Chek1, MEN1, USP7, DNMT1, FANCE, PCNA
Protein Size: 476
Molecular Weight 54 kDa
Gene ID 1111
NCBI Accession NM_001274, NP_001265
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling ProductDetails: References for anti-CHEK1 antibody (ABIN2779737) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 476 AA

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details ProductDetails: References for anti-CHEK1 antibody (ABIN2779737) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-CHEK1 antibody (ABIN2779737)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Bennett, Larkin, Gillespie, Clarke: "Claspin is phosphorylated in the Chk1-binding domain by a kinase distinct from Chk1." in: Biochemical and biophysical research communications, Vol. 369, Issue 3, pp. 973-6, 2008

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN2779737) back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (ABIN2779737) Host: Rabbit Target Name: CHEK1 Sample Type: 721_B Antibody Dilution: 1.0ug/ml CHEK1...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (ABIN2779737) Host: Rabbit Target Name: CHEK1 Sample Type: Human Adult Placenta Antibody Dilution: ...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (ABIN2779737) Host: Rabbit Target Name: CHEK1 Sample Type: Human Fetal Lung Antibody Dilution: 1.0u...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (ABIN2779737) Host: Rabbit Target Name: CHEK1 Sample Type: Human Fetal Muscle Antibody Dilution: 1....
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (ABIN2779737) Lanes : Lane 1: 40ug SH-SY5Y lysateLane 2: 40ug SH-SY5Y lysateLane 3: 40ug HEK293T ly...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (ABIN2779737) Rabbit Anti-CHEK1 antibody Catalog Number: ARP32588_P050 Paraffin Embedded Tissue: H...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (ABIN2779737) WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positiv...