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anti-Cow (Bovine) CHEK1 antibody for Western Blotting

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
Middle Region
58
48
37
34
28
20
14
13
12
10
9
6
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
451
149
139
4
4
4
2
2
1
1
1
Host
Rabbit
393
51
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
374
180
137
64
60
34
25
22
20
8
6
5
1
1
Supplier
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Available images

Catalog No. ABIN2779738
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN2779737 WB Rabbit Middle Region Log in to see Polyclonal 1
0.0011728023 ABIN3197992 IHC WB Rabbit pSer296 Log in to see Polyclonal
0.0011728023 ABIN2561835 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.0011728023 ABIN2972483 WB Rabbit Center, pSer296 Log in to see Polyclonal

Top referenced anti-CHEK1 antibody for Western Blotting

Similar anti-CHEK1 Antibodies

Application / Reactivity Human Chicken Cow (Bovine) Dog (Canine) Mouse (Murine) Rat (Rattus) Xenopus laevis Zebrafish (Danio rerio)
Western Blotting (WB) 362 Antibodies 2 Antibodies 5 Antibodies 3 Antibodies 99 Antibodies 92 Antibodies 2 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 7 Antibodies
Immunoprecipitation (IP) 17 Antibodies 3 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 63 Antibodies 28 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 140 Antibodies 1 Antibodies 2 Antibodies 3 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope Middle Region
(58), (48), (37), (34), (28), (20), (14), (13), (12), (10), (9), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(451), (149), (139), (4), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(393), (51), (22), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(374), (180), (137), (64), (60), (34), (25), (22), (20), (8), (6), (5), (1), (1)
Pubmed 1 reference available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN2779738) Images
Blocking Peptide Blocking peptide for this product available: ABIN974904
Sequence WSCGIVLTAM LAGELPWDQP SDSCQEYSDW KEKKTYLNPW KKIDSAPLAL
Predicted Reactivity Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Bovine : 90 %, Chicken : 90 %, Guinea pig : 90 %, Pig : 90 %, Zebrafish : 70 %
Characteristics This is a rabbit polyclonal antibody against CHEK1. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CHEK1

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN2779738) Images back to top
Antigen
Alternative Name CHEK1 (CHEK1 Antibody Abstract)
Background CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.
Molecular Weight 54 kDa
Gene ID 1111
NCBI Accession NP_001265, NM_001274
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN2779738) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 476 AA

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN2779738) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHEK1 Antibody (ABIN2779738)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Krämer, Mailand, Lukas, Syljuåsen, Wilkinson, Nigg, Bartek, Lukas: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN2779738) back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779738) WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/ml
Positive Control...
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779738) anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (Image 2)