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anti-Human CHEK1 antibody for Immunocytochemistry

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
Internal Region
58
48
37
33
28
19
14
13
12
10
9
6
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
446
147
137
5
4
4
3
2
2
2
1
1
Host
Rabbit
390
50
21
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
370
180
135
60
57
34
22
22
19
8
6
4
1
1
Supplier
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Catalog No. ABIN2857050
€ 341.52
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010086455 ABIN189257 ICC FACS IF IHC (p) IHC WB Rabbit IgG C-Term Log in to see EP691Y
0.0010086455 ABIN2857049 ICC IF WB Rabbit IgG Internal Region, Ser345 Log in to see Polyclonal
0.0010086455 ABIN151911 ICC IF IHC (p) IHC PLA WB Rabbit IgG AA 250-300 Log in to see Polyclonal
0.0010086455 ABIN2857048 ICC IF IHC (p) WB Rabbit IgG Internal Region, pSer345 Log in to see Polyclonal
0.0010086455 ABIN1821001 ICC IHC (p) Mouse IgG2b Log in to see DCS-310-1
0.0010086455 ABIN445205 ICC IF IHC WB Rabbit IgG Ser296 Log in to see EPR915
0.0010086455 ABIN439073 ICC IF IHC (p) IHC WB Rabbit Gly282 Log in to see Polyclonal
0.0010086455 ABIN2883630 ICC ELISA WB Rabbit IgG pSer301 Log in to see Polyclonal
0.0010086455 ABIN2594897 ICC Rabbit Internal Region Log in to see Polyclonal
0.0010086455 ABIN2594896 ICC Rabbit Internal Region Log in to see Polyclonal
0.0010086455 ABIN2883628 ICC ELISA WB Rabbit IgG pSer286 Log in to see Polyclonal
0.0010086455 ABIN407619 ICC IF IHC (p) IHC WB Rabbit IgG Asp311 Log in to see Polyclonal
0.0010086455 ABIN2594893 ICC Rabbit Internal Region Log in to see Polyclonal
0.0010086455 ABIN1977789 ICC IF IP IHC (p) PLA WB Rabbit IgG AA 250-300 Log in to see Polyclonal
0.0010086455 ABIN4297950 ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 2G1D5
0.0010086455 ABIN1843350 ICC IF WB Rabbit pSer286 Log in to see Polyclonal
0.0010086455 ABIN1843354 ICC IF WB Rabbit pSer301 Log in to see Polyclonal
0.0010086455 ABIN1843352 ICC IF WB Rabbit Log in to see Polyclonal
0.0010086455 ABIN4297952 ICC IF IHC WB Rabbit pSer317 Log in to see Polyclonal
0.0010086455 ABIN2987163 ICC IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal

Similar anti-CHEK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 22 Antibodies
ELISA 164 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunofluorescence (IF) 63 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 140 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 63 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Proximity Ligation Assay (PLA) 7 Antibodies
Western Blotting (WB) 362 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope Internal Region
(58), (48), (37), (33), (28), (19), (14), (13), (12), (10), (9), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(446), (147), (137), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(390), (50), (21), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(370), (180), (135), (60), (57), (34), (22), (22), (19), (8), (6), (4), (1), (1)
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Cross-Reactivity (Details) Mouse (85 %), Rat (92 %), Bovine (92 %)
Characteristics Rabbit Polyclonal antibody to Chk1 (CHK1 checkpoint homolog (S. pombe))
Chk1 antibody [C1C2-6], Internal
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Chk1. The exact sequence is proprietary.
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Molecular Weight 54 kDa
Gene ID 1111
Research Area Cancer, Cell Cycle, DNA/RNA, Kinases/Phosphatases
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*
Comment

Positive Control: human Chk1-transfected 293T

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Internal Region) (ABIN2857050) WB Image Chk1 antibody [C1C2-6], Internal detects Chk1 protein by western blot analys...
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Internal Region) (ABIN2857050) IHC-P Image Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft, using ...
Immunofluorescence (IF) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Internal Region) (ABIN2857050) ICC/IF Image CHK1 antibody [C1C2-6], Internal detects Chk1 protein at nucleus by immu...