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anti-Human CHEK1 antibody for Immunohistochemistry

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
pSer296
62
50
36
34
29
15
15
13
12
10
9
6
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
469
200
174
14
13
8
8
8
8
4
4
4
3
3
2
1
1
1
1
Host
Rabbit
404
56
24
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
396
184
143
71
67
40
23
22
22
9
7
7
6
2
Options
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Catalog No. ABIN1531427
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.72946 ABIN2779738 IHC WB Rabbit Middle Region Log in to see Polyclonal 1
8.72946 ABIN1532443 IHC ELISA Rabbit IgG Log in to see Polyclonal 1
8.72946 ABIN439073 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 2
8.72946 ABIN1531801 IHC ELISA Rabbit IgG pSer345 Log in to see Polyclonal
8.72946 ABIN1532830 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
8.72946 ABIN2705865 IHC WB Rabbit pSer317 Log in to see Polyclonal
8.72946 ABIN2705868 IC IF IHC WB Rabbit Center Log in to see Polyclonal
8.72946 ABIN2705867 IC IF IHC WB Rabbit Center Log in to see Polyclonal
8.72946 ABIN2705869 IC IF IHC WB Rabbit Center Log in to see Polyclonal
8.72946 ABIN2163687 IHC ELISA WB Rabbit IgG Ser280 Log in to see Polyclonal
8.72946 ABIN2163689 IHC Rabbit IgG Ser345 Log in to see Polyclonal
8.72946 ABIN2163692 IHC ELISA WB Rabbit IgG Ser317 Log in to see Polyclonal
8.72946 ABIN2163691 IHC ELISA WB Rabbit IgG Ser317 Log in to see Polyclonal
8.72946 ABIN2163693 IHC WB Rabbit IgG Ser317 Log in to see Polyclonal
8.72946 ABIN2469671 IHC WB Rabbit IgG Ser280 Log in to see Polyclonal
8.72946 ABIN2160085 IHC WB Rabbit Ig Fraction pSer280 Log in to see Polyclonal
8.72946 ABIN4297946 IHC IHC (p) WB Rabbit pSer345 Log in to see Polyclonal
8.72946 ABIN2160084 DB IHC WB Rabbit Ig Fraction pSer317 Log in to see Polyclonal
8.72946 ABIN2163670 IHC ELISA Rabbit IgG Ser345 Log in to see Polyclonal
8.72946 ABIN2163674 IHC ELISA WB Rabbit IgG Ser317 Log in to see Polyclonal

Top referenced anti-CHEK1 antibody for Immunohistochemistry

Similar anti-CHEK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 22 Antibodies
ELISA 169 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 67 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 142 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 69 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Proximity Ligation Assay (PLA) 7 Antibodies
Western Blotting (WB) 378 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope pSer296
(62), (50), (36), (34), (29), (15), (15), (13), (12), (10), (9), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(469), (200), (174), (14), (13), (8), (8), (8), (8), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(404), (56), (24), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(396), (184), (143), (71), (67), (40), (23), (22), (22), (9), (7), (7), (6), (2)
Pubmed 1 reference available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN1531427) Images
Specificity Chk1 (Phospho-Ser296) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser296.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser296.
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN1531427) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Molecular weight of target protein: 54 kDa
Synonyms: CHEK1, Chk1, kinase Chk1
Molecular Weight 54 kDa
Gene ID 1111
OMIM 603078
UniProt O14757
Research Area Cancer, Cell Cycle, DNA/RNA, Kinases/Phosphatases
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN1531427) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.24529 (NCBI Gene Symbol: CHK1)

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN1531427) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-CHEK1 Antibody (ABIN1531427)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Sanchez, Wong, Thoma, Richman, Wu, Piwnica-Worms, Elledge: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN1531427) back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer296) (ABIN1531427) Western blot analysis of extracts from HuvEc cells treated with UV 15', using Chk1 (P...
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer296) (ABIN1531427) Immunohistochemistry analysis of paraffin-embedded human brain, using Chk1 (Phospho-S...