anti-Human CHEK1 antibody for Immunohistochemistry

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
Middle Region
60
50
36
32
30
16
15
14
12
9
9
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
459
195
166
14
13
9
9
9
7
4
4
3
3
2
1
1
1
Host
Rabbit
396
54
23
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
389
170
133
73
65
40
22
21
21
10
7
6
6
2
Options
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Catalog No. ABIN2779738
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.742401 ABIN2779737 IHC WB Rabbit Middle Region Log in to see Polyclonal 1
9.742401 ABIN1531427 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal 1
9.742401 ABIN1532443 IHC ELISA Rabbit IgG Log in to see Polyclonal 1
9.742401 ABIN439073 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 2
9.742401 ABIN1531801 IHC ELISA Rabbit IgG pSer345 Log in to see Polyclonal
9.742401 ABIN1532830 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.742401 ABIN2705868 IC IF IHC WB Rabbit Center Log in to see Polyclonal
9.742401 ABIN2705867 IC IF IHC WB Rabbit Center Log in to see Polyclonal
9.742401 ABIN2705869 IC IF IHC WB Rabbit Center Log in to see Polyclonal
9.742401 ABIN2705865 IHC WB Rabbit pSer317 Log in to see Polyclonal
9.742401 ABIN2469671 IHC WB Rabbit IgG Ser280 Log in to see Polyclonal
9.742401 ABIN4297946 IHC IHC (p) WB Rabbit pSer345 Log in to see Polyclonal
9.742401 ABIN4297952 ICC IF IHC WB Rabbit pSer317 Log in to see Polyclonal
9.742401 ABIN2736519 IHC WB Rabbit IgG Log in to see Polyclonal
9.742401 ABIN4297947 IHC IHC (p) WB Rabbit Log in to see Polyclonal
9.742401 ABIN3030470 IHC ELISA WB Rabbit Ig Fraction pSer280 Log in to see Polyclonal
9.742401 ABIN634171 IHC WB Rabbit Log in to see Polyclonal
9.742401 ABIN1847310 IHC ELISA WB Rabbit Internal Region, pSer296 Log in to see Polyclonal
1 ABIN151911 ICC IF IHC (p) IHC PLA WB Rabbit IgG AA 250-300 Log in to see Polyclonal 5
1 ABIN4297951 IHC IHC (p) WB Rabbit pSer317 Log in to see Polyclonal 3

Top referenced anti-CHEK1 antibody for Immunohistochemistry

Similar anti-CHEK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Zebrafish Horse (Equine) Rabbit Guinea Pig
Immunofluorescence (fixed cells) (IF/ICC) 10 Antibodies 5 Antibodies 4 Antibodies
Western Blotting (WB) 372 Antibodies 10 Antibodies 13 Antibodies 140 Antibodies 117 Antibodies 3 Antibodies 10 Antibodies 10 Antibodies 5 Antibodies
Proximity Ligation Assay (PLA) 6 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope Middle Region
(60), (50), (36), (32), (30), (16), (15), (14), (12), (9), (9), (6), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(459), (195), (166), (14), (13), (9), (9), (9), (7), (4), (4), (3), (3), (2), (1), (1), (1)
Host Rabbit
(396), (54), (23), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(389), (170), (133), (73), (65), (40), (22), (21), (21), (10), (7), (6), (6), (2)
Pubmed 1 reference available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN2779738) Images
Blocking Peptide Blocking peptide for this product available: ABIN974904
Sequence WSCGIVLTAM LAGELPWDQP SDSCQEYSDW KEKKTYLNPW KKIDSAPLAL
Predicted Reactivity Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against CHEK1. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CHEK1

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN2779738) Images back to top
Antigen
Alternative Name CHEK1 (CHEK1 Antibody Abstract)
Background CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.
Alias Symbols: CHK1
Protein Interaction Partner: RELA, CUL4A, UBC, SETMAR, DDB1, TNRC6B, ATR, HECW2, CLSPN, DTL, BTG3, TOPBP1, MAPKAPK2, HSP90AA1, CDC25C, RAD51, PIGM, CUL1, VCP, CDC25A, ATM, APP, MED15, NEK2, TLK1, RB1, BRCA1, FEM1B, XIAP, COPS5, MEPE, LATS2, TP53, Chek1, MEN1, USP7, DNMT1, FANCE, PCNA
Protein Size: 476
Molecular Weight 54 kDa
Gene ID 1111
NCBI Accession NM_001274, NP_001265
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling ProductDetails: References for anti-CHEK1 antibody (ABIN2779738) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 476 AA

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details ProductDetails: References for anti-CHEK1 antibody (ABIN2779738) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-CHEK1 antibody (ABIN2779738)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Krämer, Mailand, Lukas, Syljuåsen, Wilkinson, Nigg, Bartek, Lukas: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN2779738) back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779738) WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/ml
Positive Control...
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779738) anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (Image 2)