anti-Human CHEK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
pSer317
70
66
39
33
16
15
15
15
14
12
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
486
195
167
14
14
10
10
10
7
5
4
4
3
2
1
1
1
Host
Rabbit
422
55
23
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
411
178
143
75
69
40
25
21
21
10
7
6
6
5
Supplier
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Catalog No. ABIN4297951
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.588177 ABIN151911 ICC IF IHC (p) IHC PLA WB Rabbit IgG AA 250-300 Log in to see Polyclonal 5
13.588177 ABIN214737 IHC (p) WB Rabbit AA 192-241 Log in to see Polyclonal
13.588177 ABIN4297946 IHC IHC (p) WB Rabbit pSer345 Log in to see Polyclonal
13.588177 ABIN447119 IHC (p) IHC Rabbit pSer345 Log in to see Polyclonal
13.588177 ABIN407619 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
13.588177 ABIN214042 IHC (p) WB Rabbit IgG pSer280 Log in to see Polyclonal
13.588177 ABIN498255 IF IHC (p) WB Rabbit Log in to see Polyclonal
13.588177 ABIN498827 IHC (p) Rabbit pSer345 Log in to see Polyclonal
13.588177 ABIN498828 IHC (p) Rabbit pSer317 Log in to see Polyclonal
13.588177 ABIN498916 IHC (p) Rabbit Log in to see Polyclonal
13.588177 ABIN498915 IHC (p) Rabbit Log in to see Polyclonal
1 ABIN358109 EIA IHC (p) WB Rabbit Ig pSer317 Log in to see Polyclonal 5
1 ABIN358108 EIA IHC (p) WB Rabbit Ig pSer280 Log in to see Polyclonal 5
1 ABIN757072 IF (p) IHC (p) WB Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal 1
1 ABIN389543 DB IHC (p) WB Rabbit Ig pSer317 Log in to see Polyclonal 3
1 ABIN389542 IHC (p) WB Rabbit Ig pSer280 Log in to see Polyclonal 3
1 ABIN197292 IHC (p) WB Rabbit Ser317 Log in to see Polyclonal 2
1 ABIN439073 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 2
1 ABIN487487 IHC (p) IP WB Mouse IgG2b Log in to see DCS-310
1 ABIN2857050 ICC IF IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope pSer317
(70), (66), (39), (33), (16), (15), (15), (15), (14), (12), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(486), (195), (167), (14), (14), (10), (10), (10), (7), (5), (4), (4), (3), (2), (1), (1), (1)
Host Rabbit
(422), (55), (23), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(411), (178), (143), (75), (69), (40), (25), (21), (21), (10), (7), (6), (6), (5)
Pubmed 3 references available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN4297951) Images
Purification Immunogen affinity purified
Immunogen Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 317.

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 antibody (ABIN4297951) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Gene Symbol: CHEK1
Molecular Weight Theoretical MW: 56 kDa
Gene ID 1111
UniProt O14757
Research Area DNA/RNA, Kinases/Phosphatases, Cell Cycle, Cancer
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 antibody (ABIN4297951) Images back to top
Application Notes Western Blot 1:500-1:1000, Immunohistochemistry 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200Western blot (WB) analysis of p-Chk1 (S317) pAb in extracts from MCF7 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 antibody (ABIN4297951) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.2)
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CHEK1 antibody (ABIN4297951)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Product cited in:

Bernard, Lou, Peng, Li, Conney, Lu: "Inverse relationship between p53 and phospho-Chk1 (Ser317) protein expression in UVB-induced skin tumors in SKH-1 mice." in: Experimental and molecular pathology, Vol. 96, Issue 1, pp. 126-31, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Zhou, Chen, Malysa, Li, Oliveira, Zhang, Bepler: "A kinome screen identifies checkpoint kinase 1 (CHK1) as a sensitizer for RRM1-dependent gemcitabine efficacy." in: PLoS ONE, Vol. 8, Issue 3, pp. e58091, 2013 (Sample species: Human). Further details: Immunohistochemistry

Walluscheck, Poehlmann, Hartig, Lendeckel, Schönfeld, Hotz-Wagenblatt, Reissig, Bajbouj, Roessner, Schneider-Stock: "ATF2 knockdown reinforces oxidative stress-induced apoptosis in TE7 cancer cells." in: Journal of cellular and molecular medicine, Vol. 17, Issue 8, pp. 976-88, 2013 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN4297951) back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN4297951) Western Blot: Chk1 [p Ser317] Antibody [ABIN42979519] - p-Chk1 (S317) antibody in ext...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN4297951) Western Blot: Chk1 [p Ser317] Antibody [ABIN42979519] - Lane1:HEK293T cell lysate. La...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN4297951) Immunohistochemistry-Paraffin: Chk1 [p Ser317] Antibody [ABIN42979519] - Paraffin-emb...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN4297951) Western Blot: Chk1 [p Ser317] Antibody [ABIN42979519] - Analysis of p-Chk1 (S317) pAb...