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anti-Human CHEK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
AA 259-311
58
48
37
34
28
20
14
13
12
10
9
6
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
451
150
140
5
4
4
3
2
2
2
1
1
Host
Rabbit
393
51
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
374
180
138
63
59
34
25
22
20
8
6
5
1
1
Supplier
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Catalog No. ABIN497950
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34288716 ABIN498255 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.34288716 ABIN214737 IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
0.34288716 ABIN498827 IHC (p) Rabbit pSer345 Log in to see Polyclonal
0.34288716 ABIN214042 IHC (p) WB Rabbit IgG Ser280 Log in to see Polyclonal
0.34288716 ABIN498828 IHC (p) Rabbit pSer317 Log in to see Polyclonal
0.34288716 ABIN498916 IHC (p) Rabbit Log in to see Polyclonal
0.34288716 ABIN498915 IHC (p) Rabbit Log in to see Polyclonal
0.0008982679 ABIN358109 EIA IHC (p) WB Rabbit Ig pSer317 Log in to see Polyclonal 5
0.0008982679 ABIN358108 EIA IHC (p) WB Rabbit Ig pSer280 Log in to see Polyclonal 5
0.0008982679 ABIN389543 DB IHC (p) WB Rabbit Ig pSer317 Log in to see Polyclonal 3
0.0008982679 ABIN389542 IHC (p) WB Rabbit Ig pSer280 Log in to see Polyclonal 3
0.0008982679 ABIN197292 IHC (p) WB Rabbit Ser317 Log in to see Polyclonal 2
0.0008982679 ABIN265347 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN265348 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN487487 IHC (p) IP WB Mouse IgG2b Log in to see DCS-310
0.0008982679 ABIN302922 IHC (p) WB Rabbit IgG pSer280 Log in to see Polyclonal
0.0008982679 ABIN2857050 ICC IF IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0008982679 ABIN317989 IHC (p) Rabbit pSer345 Log in to see Polyclonal
0.0008982679 ABIN317988 IHC (p) WB Rabbit pSer317 Log in to see Polyclonal
0.0008982679 ABIN189257 ICC FACS IF IHC (p) IHC WB Rabbit IgG C-Term Log in to see EP691Y

Top referenced anti-CHEK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

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Application / Reactivity Human
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ELISA 164 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunofluorescence (IF) 63 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 140 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 63 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Proximity Ligation Assay (PLA) 7 Antibodies
Western Blotting (WB) 362 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope AA 259-311
(58), (48), (37), (34), (28), (20), (14), (13), (12), (10), (9), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(451), (150), (140), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(393), (51), (22), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(374), (180), (138), (63), (59), (34), (25), (22), (20), (8), (6), (5), (1), (1)
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling Images
Specificity Recognizes CHK1 (259-311).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Antigen-Affinity Chromatography.
Immunogen Synthetic peptide contain a sequence corresponding to a region within amino acids 259 and 311 of CHK1.

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CHK1 (CHEK1 Antibody Abstract)
Background Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). Chk1 plays an essential role in the mammalian DNA damage checkpoint.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1
Molecular Weight 54 kDa
Gene ID 1111, 9606
UniProt O14757
Research Area Kinases/Phosphatases, Cell Cycle
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Application Notes Western blotting: 1/500-1/3000. Immunocytochemistry. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/100-1/500. Positive Controls: 293T, A431, H1299, Molt-4, Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 1xPBS, 1 % BSA, 20 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C.

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 259-311) (ABIN497950) AP19049PU-N CHK1 antibody staining of Paraformaldehyde-fixed HeLa by Immunofluorescen...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 259-311) (ABIN497950) AP19049PU-N CHK1 antibody staining of Paraffin-Embedded SCM-1 xenograft at 1/100 dilu...
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 259-311) (ABIN497950) AP19049PU-N CHK1 antibody staining of 293T nuclear extract (A) 7.5% SDS PAGE (20 µg) ...