anti-Human CHEK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
AA 250-300
61
50
36
33
30
16
15
14
12
9
9
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
466
195
166
14
14
10
10
10
7
5
4
4
3
2
1
1
1
Host
Rabbit
402
55
23
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Proximity Ligation Assay (PLA), Western Blotting (WB)
396
172
133
76
65
40
21
21
21
10
7
6
5
1
Supplier
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Catalog No. ABIN151911
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.399764 ABIN4297951 IHC IHC (p) WB Rabbit pSer317 Log in to see Polyclonal 3
13.399764 ABIN497950 IF IHC (p) WB Rabbit AA 259-311 Log in to see Polyclonal
13.399764 ABIN498255 IF IHC (p) WB Rabbit Log in to see Polyclonal
13.399764 ABIN214737 IHC (p) WB Rabbit AA 192-241 Log in to see Polyclonal
13.399764 ABIN4297946 IHC IHC (p) WB Rabbit pSer345 Log in to see Polyclonal
13.399764 ABIN447119 IHC (p) IHC Rabbit pSer345 Log in to see Polyclonal
13.399764 ABIN407619 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
13.399764 ABIN498827 IHC (p) Rabbit pSer345 Log in to see Polyclonal
13.399764 ABIN214042 IHC (p) WB Rabbit IgG Ser280 Log in to see Polyclonal
13.399764 ABIN498915 IHC (p) Rabbit Log in to see Polyclonal
13.399764 ABIN498916 IHC (p) Rabbit Log in to see Polyclonal
13.399764 ABIN498828 IHC (p) Rabbit pSer317 Log in to see Polyclonal
1 ABIN358109 EIA IHC (p) WB Rabbit Ig pSer317 Log in to see Polyclonal 5
1 ABIN358108 EIA IHC (p) WB Rabbit Ig pSer280 Log in to see Polyclonal 5
1 ABIN757072 IF (p) IHC (p) WB Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal 1
1 ABIN389543 DB IHC (p) WB Rabbit Ig pSer317 Log in to see Polyclonal 3
1 ABIN389542 IHC (p) WB Rabbit Ig pSer280 Log in to see Polyclonal 3
1 ABIN197292 IHC (p) WB Rabbit Ser317 Log in to see Polyclonal 2
1 ABIN439073 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 2
1 ABIN265348 IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-CHEK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-CHEK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 21 Antibodies
ELISA 157 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 66 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 132 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 75 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Proximity Ligation Assay (PLA) 6 Antibodies
Western Blotting (WB) 379 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope AA 250-300
(61), (50), (36), (33), (30), (16), (15), (14), (12), (9), (9), (6), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(466), (195), (166), (14), (14), (10), (10), (10), (7), (5), (4), (4), (3), (2), (1), (1), (1)
Host Rabbit
(402), (55), (23), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Proximity Ligation Assay (PLA), Western Blotting (WB)
(396), (172), (133), (76), (65), (40), (21), (21), (21), (10), (7), (6), (5), (1)
Pubmed 5 references available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN151911) Images
Purification Immunogen affinity purified
Immunogen The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in SwissProt entry O14757 (GeneID 1111).
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN151911) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Gene Symbol: CHEK1
Molecular Weight Theoretical MW: 54 kDa
Gene ID 1111
UniProt O14757
Research Area DNA/RNA, Kinases/Phosphatases, Cell Cycle, Cancer
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling ProductDetails: References for anti-CHEK1 antibody (ABIN151911) Images back to top
Application Notes Western Blot 1:10000-1:25000, Immunohistochemistry 1:1000-1:5000, Immunocytochemistry/Immunofluorescence 1:500-1:2000, Immunohistochemistry-Paraffin 1:1000-1:5000, Proximity Ligation Assay 1:1000-1:10000The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details ProductDetails: References for anti-CHEK1 antibody (ABIN151911) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

ProductDetails: References for anti-CHEK1 antibody (ABIN151911)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Product cited in:

Jazaeri, Shibata, Park, Bryant, Conaway, Modesitt, Smith, Milhollen, Berger, Dutta: "Overcoming platinum resistance in preclinical models of ovarian cancer using the neddylation inhibitor MLN4924." in: Molecular cancer therapeutics, Vol. 12, Issue 10, pp. 1958-67, 2013 (Sample species: Human). Further details: Western Blotting

Terai, Shibata, Abbas, Dutta: "Degradation of p12 subunit by CRL4Cdt2 E3 ligase inhibits fork progression after DNA damage." in: The Journal of biological chemistry, Vol. 288, Issue 42, pp. 30509-14, 2013 (Sample species: Human). Further details: Western Blotting

Hong, Hu, Yuan, Sang, Bu, Chen, Yang, Li, Huang, Chen, Liang, Zhang, Pan, Zeng, Kang: "CHK1 targets spleen tyrosine kinase (L) for proteolysis in hepatocellular carcinoma." in: The Journal of clinical investigation, Vol. 122, Issue 6, pp. 2165-75, 2012 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry

Karnani, Dutta: "The effect of the intra-S-phase checkpoint on origins of replication in human cells." in: Genes & development, Vol. 25, Issue 6, pp. 621-33, 2011

Shiotani, Kobayashi, Watanabe, Yamamoto, Sugimura, Wakabayashi: "Involvement of the ATR- and ATM-dependent checkpoint responses in cell cycle arrest evoked by pierisin-1." in: Molecular cancer research : MCR, Vol. 4, Issue 2, pp. 125-33, 2006

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN151911) back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 250-300) (ABIN151911) Detection of Chk1 in HeLa whole cell lysates using ABIN151911.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 250-300) (ABIN151911) Immunohistochemistry-Paraffin: Chk1 Antibody [ABIN151911] - Human testicular seminoma...
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 250-300) (ABIN151911) Western Blot: Chk1 Antibody [ABIN151911] - Whole cell lysate (20 ug) from HeLa cells....
Proximity Ligation Assay (PLA) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 250-300) (ABIN151911) Proximity Ligation Assay: Chk1 Antibody [ABIN151911] - Secondary-conjugate Duolink II...
Proximity Ligation Assay (PLA) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 250-300) (ABIN151911) Proximity Ligation Assay: Chk1 Antibody [ABIN151911] - Secondary-conjugate Duolink In...
Proximity Ligation Assay (PLA) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 250-300) (ABIN151911) Proximity Ligation Assay: Chk1 Antibody [ABIN151911] - Secondary-conjugate Duolink In...
Proximity Ligation Assay (PLA) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 250-300) (ABIN151911) Proximity Ligation Assay: Chk1 Antibody [ABIN151911] - Secondary-conjugate Duolink II...
Proximity Ligation Assay (PLA) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 250-300) (ABIN151911) Proximity Ligation Assay: Chk1 Antibody [ABIN151911] - Secondary-conjugate Duolink in...
Immunofluorescence (IF) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 250-300) (ABIN151911) Immunocytochemistry/Immunofluorescence: Chk1 Antibody [ABIN151911] - NBF-fixed HeLa c...