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anti-Human CHEK1 antibody for Western Blotting

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
Middle Region
56
47
37
32
28
20
14
13
12
10
9
6
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
431
137
128
4
4
3
3
2
1
1
1
1
Host
Rabbit
377
47
21
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
12
12
11
8
8
8
3
3
3
3
3
3
3
3
3
Application
Western Blotting (WB)
354
173
125
56
52
34
22
21
18
8
6
4
1
1
Supplier
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Catalog No. ABIN2779737
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13314965 ABIN2779738 IHC WB Rabbit Middle Region Log in to see Polyclonal 1
0.13314965 ABIN1531555 IF ELISA WB Rabbit IgG pSer301 Log in to see Polyclonal 2
0.13314965 ABIN1531554 IF ELISA WB Rabbit IgG pSer286 Log in to see Polyclonal 2
0.13314965 ABIN1531427 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal 1
0.13314965 ABIN389542 IHC (p) WB Rabbit Ig pSer280 Log in to see Polyclonal 3
0.13314965 ABIN389543 DB IHC (p) WB Rabbit Ig pSer317 Log in to see Polyclonal 3
0.13314965 ABIN1532570 IF ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.13314965 ABIN387806 WB Mouse IgM Log in to see 2G1D5 2
0.13314965 ABIN969476 ELISA WB Mouse IgG1 Log in to see 2G1D5 2
0.13314965 ABIN388983 WB Rabbit Ig AA 258-287, Ser280 Log in to see Polyclonal 2
0.13314965 ABIN487487 IHC (p) IP WB Mouse IgG2b Log in to see DCS-310
0.13314965 ABIN1106682 EIA WB Mouse IgG1 Log in to see 2G1D5 1
0.13314965 ABIN497950 IF IHC (p) WB Rabbit AA 259-311 Log in to see Polyclonal
0.13314965 ABIN1531800 ELISA WB Rabbit IgG pSer317 Log in to see Polyclonal
0.13314965 ABIN1531799 ELISA WB Rabbit IgG pSer280 Log in to see Polyclonal
0.13314965 ABIN2857050 ICC IF IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
0.13314965 ABIN1532831 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13314965 ABIN1532830 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13314965 ABIN1532829 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13314965 ABIN2857049 ICC IF WB Rabbit IgG Internal Region, Ser345 Log in to see Polyclonal

Top referenced anti-CHEK1 antibody for Western Blotting

Similar anti-CHEK1 Antibodies

Application / Reactivity Human Chicken Cow (Bovine) Mouse (Murine) Rat (Rattus) Xenopus laevis
Western Blotting (WB) 347 Antibodies 2 Antibodies 5 Antibodies 89 Antibodies 83 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 7 Antibodies
Immunoprecipitation (IP) 17 Antibodies 3 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 54 Antibodies 21 Antibodies 16 Antibodies
Immunohistochemistry (IHC) 130 Antibodies 1 Antibodies 2 Antibodies 33 Antibodies 39 Antibodies 1 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope Middle Region
(56), (47), (37), (32), (28), (20), (14), (13), (12), (10), (9), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(431), (137), (128), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(377), (47), (21), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(12), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(354), (173), (125), (56), (52), (34), (22), (21), (18), (8), (6), (4), (1), (1)
Pubmed 1 reference available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN2779737) Images
Blocking Peptide Blocking peptide for this product available: ABIN974903
Sequence SARITIPDIK KDRWYNKPLK KGAKRPRVTS GGVSESPSGF SKHIQSNLDF
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Bovine : 90 %
Characteristics This is a rabbit polyclonal antibody against CHEK1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CHEK1

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN2779737) Images back to top
Antigen
Alternative Name CHEK1 (CHEK1 Antibody Abstract)
Background CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.
Molecular Weight 54 kDa
Gene ID 1111
NCBI Accession NP_001265, NM_001274
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN2779737) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 476 AA

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN2779737) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHEK1 Antibody (ABIN2779737)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Bennett, Larkin, Gillespie, Clarke: "Claspin is phosphorylated in the Chk1-binding domain by a kinase distinct from Chk1." in: Biochemical and biophysical research communications, Vol. 369, Issue 3, pp. 973-6, 2008

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN2779737) back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779737) Host: Rabbit
Target Name: CHEK1
Sample Tissue: 721_B
Antibody Di...
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779737) Host: Rabbit
Target Name: CHEK1
Sample Tissue: Human Adult Placenta
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779737) Host: Rabbit
Target Name: CHEK1
Sample Tissue: Human Fetal Lung
...
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779737) Host: Rabbit
Target Name: CHEK1
Sample Tissue: Human Fetal Muscle
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779737) Lanes: Lane 1:441 µg SH-SY5Y lysate
Lane 2: 041 µg SH-SY5Y lysate
ane ...