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anti-Zebrafish CHEK1 antibody for Western Blotting

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
AA 192-241
60
49
36
30
30
15
15
12
10
9
9
6
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
445
190
162
13
11
8
7
6
6
4
3
3
2
2
2
1
1
1
1
Host
Rabbit
384
53
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
375
170
133
70
65
40
22
21
21
10
7
6
6
2
Supplier
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Catalog No. ABIN214737
$ 676.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN207301 ELISA WB Rabbit N-Term Log in to see Polyclonal

Similar anti-CHEK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Monkey Zebrafish Bat Horse (Equine) Rabbit
Immunofluorescence (fixed cells) (IF/ICC) 10 Antibodies 5 Antibodies 4 Antibodies
Western Blotting (WB) 358 Antibodies 11 Antibodies 135 Antibodies 113 Antibodies 13 Antibodies 2 Antibodies 4 Antibodies 7 Antibodies 7 Antibodies
Proximity Ligation Assay (PLA) 6 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 18 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope AA 192-241
(60), (49), (36), (30), (30), (15), (15), (12), (10), (9), (9), (6), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(445), (190), (162), (13), (11), (8), (7), (6), (6), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(384), (53), (22), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(375), (170), (133), (70), (65), (40), (22), (21), (21), (10), (7), (6), (6), (2)
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling Images
Brand IHC-plus™
Specificity Human CHEK1 / CHK1
Predicted Reactivity Percent identity by BLAST analysis: Human, Mouse, Dog, Horse (100%) Bovine, Pig, Guinea pig, Chicken (92%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide located between aa192-241 of human CHEK1 (O14757, NP_001265). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Horse, Zebra finch (100%), Hamster, Bovine, Pig, Opossum, Guinea pig, Chicken, Platypus (92%), Stickleback (85%).

Type of Immunogen: Synthetic peptide

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CHEK1 / CHK1 (CHEK1 Antibody Abstract)
Background Name/Gene ID: CHEK1
Subfamily: NIM1
Family: Protein Kinase

Synonyms: CHEK1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase 1, CHK1, Chk1-S, Protein kinase chk1, Checkpoint kinase-1
Gene ID 1111
NCBI Accession NP_001265
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Application Notes Approved: IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
Format Lyophilized
Reconstitution After adding water, will consist of PBS buffer with 2 % sucrose
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 192-241) (ABIN214737) Human Thymus (formalin-fixed, paraffin-embedded) stained with CHEK1 antibody ABIN2147...