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|Application / Reactivity||Human||Cow (Bovine)|
|Western Blotting (WB)||363 Antibodies||7 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Luminex Assay (LMNX)||2 Antibodies|
|Immunoprecipitation (IP)||39 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||76 Antibodies|
|Immunohistochemistry (IHC)||128 Antibodies||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||56 Antibodies|
|Immunofluorescence (IF)||83 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||46 Antibodies||1 Antibodies|
|Immunochromatography (IC)||6 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||25 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Antigen||Checkpoint Kinase 2 (CHEK2) Antibodies|
|Reactivity||Cow (Bovine), Human Alternatives|
Western Blotting (WB)
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Recognizes CHK2 (422-551).
Cellular Localization: Nucleus (Isoforms 2, 4, 7, 9 and 12).
Species reactivity (expected):Bovine (87 %).
Species reactivity (tested):Human.
|Immunogen||Recombinant protein fragment contain a sequence corresponding to a region within amino acids 422 and 551 of Human CHK2|
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|Alternative Name||CHK2 (CHEK2 Antibody Abstract)|
|Background||Checkpoint kinase 2 (Chk2), also known as Cds1, is a 61 kDa nuclear protein that functions as a checkpoint kinase to regulate the cell cycle progression following DNA damage. Chk2 inhibits CDC2 by inactivating CDC25, the phosphatase that normally activates CDC2. Other targets for Chk2 include the tumor suppressors BRCA1 and p53, which it stabilizes by phosphorylation of Ser20. Chk2 is itself phosphorylated and activated by ATM following DNA damage. Defects in Chk2 contribute to the development of human cancers, and implicate Chk2 as a candidate tumor suppressor and an attractive target for drug discovery.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2|
|Molecular Weight||65 kDa|
|Gene ID||11200, 9606|
|Pathways||p53 Signaling, Apoptosis, Cell Division Cycle|
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Western blotting: 1/500-1/3000. Positive Control: Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||1xPBS, 10 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.|
|Precaution of Use||This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at -20 °C.|
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