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anti-Cow (Bovine) Checkpoint Kinase 2 antibody for Western Blotting

Recommended Checkpoint Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • Cds1
  • CDS1
  • CHK-2
  • Chk2
  • CHK2
  • fa66f08
  • hCds1
  • HuCds1
  • HUCDS1
  • LFS2
  • PP1425
  • Rad53
  • RAD53
  • wu:fa66f08
  • zgc:55865
Epitope
C-Term
44
42
40
32
23
15
13
13
12
11
10
9
9
7
6
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human
454
186
167
10
9
8
8
6
5
1
1
1
Host
Rabbit
332
128
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
7
7
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Western Blotting (WB)
372
127
124
82
75
56
45
40
25
6
5
2
1
1
Supplier
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Catalog No. ABIN497951
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398065 ABIN2705870 IHC WB Rabbit pSer387 Log in to see Polyclonal
0.13398065 ABIN2705871 IC IF WB Rabbit pSer383 Log in to see Polyclonal
0.0011731816 ABIN3197994 IHC WB Rabbit Log in to see Polyclonal
0.0011731816 ABIN2972735 WB Rabbit Center Log in to see Polyclonal
0.0011731816 ABIN2971453 ICC IF WB Rabbit Center, pThr383 Log in to see Polyclonal
0.0011731816 ABIN2971451 IHC WB Rabbit Center, pThr387 Log in to see Polyclonal

Similar anti-Checkpoint Kinase 2 Antibodies

Application / Reactivity Human Cow (Bovine)
Western Blotting (WB) 362 Antibodies 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Immunoprecipitation (IP) 39 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 75 Antibodies
Immunohistochemistry (IHC) 127 Antibodies 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunofluorescence (IF) 83 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 46 Antibodies 1 Antibodies
Immunochromatography (IC) 6 Antibodies 1 Antibodies
Flow Cytometry (FACS) 25 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope C-Term
(44), (42), (40), (32), (23), (15), (13), (13), (12), (11), (10), (9), (9), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human
(454), (186), (167), (10), (9), (8), (8), (6), (5), (1), (1), (1)
Host Rabbit
(332), (128), (8), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(8), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Western Blotting (WB)
(372), (127), (124), (82), (75), (56), (45), (40), (25), (6), (5), (2), (1), (1)
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Product details

Target details Application Details Handling Images
Specificity Recognizes CHK2 (422-551).
Cellular Localization: Nucleus (Isoforms 2, 4, 7, 9 and 12).
Cross-Reactivity (Details) Species reactivity (expected):Bovine (87 %).
Species reactivity (tested):Human.
Purification Antigen-Affinity Chromatography.
Immunogen Recombinant protein fragment contain a sequence corresponding to a region within amino acids 422 and 551 of Human CHK2

Target details

Product details Application Details Handling Images back to top
Antigen
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background Checkpoint kinase 2 (Chk2), also known as Cds1, is a 61 kDa nuclear protein that functions as a checkpoint kinase to regulate the cell cycle progression following DNA damage. Chk2 inhibits CDC2 by inactivating CDC25, the phosphatase that normally activates CDC2. Other targets for Chk2 include the tumor suppressors BRCA1 and p53, which it stabilizes by phosphorylation of Ser20. Chk2 is itself phosphorylated and activated by ATM following DNA damage. Defects in Chk2 contribute to the development of human cancers, and implicate Chk2 as a candidate tumor suppressor and an attractive target for drug discovery.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Molecular Weight 65 kDa
Gene ID 11200, 9606
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product details Target details Handling Images back to top
Application Notes Western blotting: 1/500-1/3000. Positive Control: Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1.0 mg/mL
Buffer 1xPBS, 10 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C.

Images

Product details Target details Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (C-Term) antibody (ABIN497951) AP19050PU-N CHK2 antibody staining of Raji whole cell lysate (A) 7.5% SDS PAGE (30 µg...