anti-Cow (Bovine) CHEK2 antibody for Western Blotting

Recommended CHEK2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • CHK-2
  • checkpoint kinase 2
  • CHK2 checkpoint homolog (S. pombe)
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • LOC396525
Epitope
C-Term
50
49
37
35
18
17
15
15
15
13
12
10
8
7
7
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human
471
217
198
19
19
16
13
8
5
4
2
1
1
1
1
1
1
Host
Rabbit
340
132
8
1
1
Clonality
Polyclonal
Conjugate
This CHEK2 antibody is un-conjugated
9
8
8
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Western Blotting (WB)
385
120
120
107
84
66
44
42
26
6
6
5
2
1
1
Supplier
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Catalog No. ABIN497951
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.347672 ABIN2705870 IHC WB Rabbit pSer387 Log in to see Polyclonal
3.347672 ABIN2705871 IC IF WB Rabbit pSer383 Log in to see Polyclonal
1 ABIN594244 WB Rabbit IgG pSer516 Log in to see Polyclonal
1 ABIN601012 IHC ELISA WB Rabbit IgG pThr387 Log in to see Polyclonal
1 ABIN2619239 WB Rabbit IgG AA 133-148 Log in to see Polyclonal
1 ABIN601010 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN3197994 IHC WB Rabbit Log in to see Polyclonal
1 ABIN2619241 ICC IF WB Rabbit pThr383 Log in to see Polyclonal
1 ABIN2619240 IHC (p) WB Rabbit pThr387 Log in to see Polyclonal
1 ABIN2972735 WB Rabbit Center Log in to see Polyclonal
1 ABIN2971453 IF/ICC WB Rabbit Center, pThr383 Log in to see Polyclonal
1 ABIN2971451 IHC WB Rabbit Center, pThr387 Log in to see Polyclonal

Similar anti-CHEK2 Antibodies

Application / Reactivity Human Cow (Bovine)
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies 1 Antibodies
Western Blotting (WB) 377 Antibodies 13 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Immunoprecipitation (IP) 43 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 106 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 119 Antibodies 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunofluorescence (IF) 84 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 42 Antibodies 1 Antibodies
Immunochromatography (IC) 6 Antibodies 1 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope C-Term
(50), (49), (37), (35), (18), (17), (15), (15), (15), (13), (12), (10), (8), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human
(471), (217), (198), (19), (19), (16), (13), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(340), (132), (8), (1), (1)
Clonality
Polyclonal
Conjugate This CHEK2 antibody is un-conjugated
(9), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Western Blotting (WB)
(385), (120), (120), (107), (84), (66), (44), (42), (26), (6), (6), (5), (2), (1), (1)
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Product Details anti-CHEK2 Antibody

Target Details CHEK2 Application Details Handling Images
Specificity Recognizes CHK2 (422-551).
Cellular Localization: Nucleus (Isoforms 2, 4, 7, 9 and 12).
Cross-Reactivity (Details) Species reactivity (expected):Bovine (87 %).
Species reactivity (tested):Human.
Purification Antigen-Affinity Chromatography.
Immunogen Recombinant protein fragment contain a sequence corresponding to a region within amino acids 422 and 551 of Human CHK2

Target Details CHEK2

Product Details anti-CHEK2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background Checkpoint kinase 2 (Chk2), also known as Cds1, is a 61 kDa nuclear protein that functions as a checkpoint kinase to regulate the cell cycle progression following DNA damage. Chk2 inhibits CDC2 by inactivating CDC25, the phosphatase that normally activates CDC2. Other targets for Chk2 include the tumor suppressors BRCA1 and p53, which it stabilizes by phosphorylation of Ser20. Chk2 is itself phosphorylated and activated by ATM following DNA damage. Defects in Chk2 contribute to the development of human cancers, and implicate Chk2 as a candidate tumor suppressor and an attractive target for drug discovery.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Molecular Weight 65 kDa
Gene ID 11200, 9606
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK2 Antibody Target Details CHEK2 Handling Images back to top
Application Notes Western blotting: 1/500-1/3000. Positive Control: Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 1xPBS, 10 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C.

Images

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK2 antibody (Checkpoint Kinase 2) (C-Term) (ABIN497951) AP19050PU-N CHK2 antibody staining of Raji whole cell lysate (A) 7.5% SDS PAGE (30 µg...