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anti-Chimpanzee E2F1 antibody for Western Blotting

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • ATE2FB
  • CG6376
  • dE2f
  • dE2F
  • dE2F1
  • dE2f1
  • de2f1
  • def21
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • drosE2F1
  • DRTF1/E2F
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • e2f
  • E2F
  • E2F-1
  • e2f1
  • E2f1
  • E2F1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • Evar(3)164
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • mKIAA4009
  • RBAP1
  • RBBP3
  • RBP3
  • xE2F
Epitope
AA 360-369, pSer364
31
28
27
25
24
15
13
13
10
10
9
9
9
8
8
7
6
6
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chimpanzee, Human
334
57
28
9
4
3
3
2
1
1
1
Host
Rabbit
271
52
19
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
13
13
13
3
3
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
257
158
60
41
41
37
36
36
11
10
9
2
2
1
1
1
Supplier
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Catalog No. ABIN401277
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011806369 ABIN2470299 IHC ELISA WB Rabbit pSer364 Log in to see Polyclonal
0.0011806369 ABIN2167917 IHC ELISA WB Rabbit AA 360-369 Log in to see Polyclonal

Top referenced anti-E2F1 antibody for Western Blotting

Similar anti-E2F1 Antibodies

Application / Reactivity Human Chimpanzee
Dot Blot (DB) 41 Antibodies
ELISA 152 Antibodies 2 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies 1 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Gel Shift (GS) 2 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 32 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 36 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies
Immunoprecipitation (IP) 47 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 266 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope AA 360-369, pSer364
(31), (28), (27), (25), (24), (15), (13), (13), (10), (10), (9), (9), (9), (8), (8), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chimpanzee, Human
(334), (57), (28), (9), (4), (3), (3), (2), (1), (1), (1)
Host Rabbit
(271), (52), (19), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (13), (13), (13), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(257), (158), (60), (41), (41), (37), (36), (36), (11), (10), (9), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN401277) Images
Specificity This antibody is directed against the phosphorylated form of E2F-1 at the pS364 residue. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. The antibody does not cross-react with E2F-1 phosphorylated at other sites.
Cross-Reactivity (Details) Species reactivity (expected):Chimpanzee 100 % .
Species reactivity (tested):Human.
Purification The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
Immunogen Synthetic peptide corresponding to amino acids 360-369 of Human E2F-1
Isotype IgG

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN401277) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background E2F-1 (also known as tran-scription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains con-served through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain that determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein as well as E2F2 and E2F3 have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner and mediates both cell proliferation and p53-dependent/independent apoptosis. Increased nuclear expression of this protein has been reported in a variety of cancers.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
Gene ID 1869
NCBI Accession NP_005216
UniProt Q01094
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN401277) Images back to top
Application Notes ELISA: 1: 20,000 - 1: 100,000. Western blot: 1: 250 - 1: 2,000. Immunohistochemistry on paraffin sections: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN401277) Images back to top
Concentration 0.80 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-E2F1 Antibody (ABIN401277)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Background publications

Wang, Hou, Mohapatra, Ma, Cress, Pledger, Chen: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005

Joshi, Ordonez-Ercan, Dasgupta, Chellappan: "Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors." in: Oncogene, Vol. 24, Issue 13, pp. 2204-17, 2005

Shats, Milyavsky, Tang, Stambolsky, Erez, Brosh, Kogan, Braunstein, Tzukerman, Ginsberg, Rotter: "p53-dependent down-regulation of telomerase is mediated by p21waf1." in: The Journal of biological chemistry, Vol. 279, Issue 49, pp. 50976-85, 2004

Chaussepied, Ginsberg: "Transcriptional regulation of AKT activation by E2F." in: Molecular cell, Vol. 16, Issue 5, pp. 831-7, 2004

Sdek, Ying, Zheng, Margulis, Tang, Tian, Xiao: "The central acidic domain of MDM2 is critical in inhibition of retinoblastoma-mediated suppression of E2F and cell growth." in: The Journal of biological chemistry, Vol. 279, Issue 51, pp. 53317-22, 2004

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN401277) back to top
Supplier Images
 image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 360-369) (ABIN401277) anti-E2F Transcription Factor 1 (E2F1) (AA 360-369), (pSer364) antibody
 image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 360-369) (ABIN401277) anti-E2F Transcription Factor 1 (E2F1) (AA 360-369), (pSer364) antibody (Image 2)