anti-Human E2F1 antibody for Dot Blot

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Epitope
pHis357
31
30
30
27
25
15
14
14
12
11
10
10
9
9
8
8
7
6
6
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
371
102
47
10
8
6
6
5
5
4
3
3
2
1
1
Host
Rabbit
286
73
21
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
21
19
16
14
13
13
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB)
291
166
78
60
58
55
42
38
25
13
12
3
2
2
1
1
1
Options
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Catalog No. ABIN650836
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
29.509521 ABIN389869 DB Rabbit Ig pSer332 Log in to see Polyclonal 5
29.509521 ABIN3028630 DB ELISA Rabbit Ig Fraction pHis357 Log in to see Polyclonal
29.509521 ABIN3028631 DB ELISA Rabbit Ig Fraction pSer332 Log in to see Polyclonal
1 ABIN932458 DB ICC Mouse IgG2b Log in to see 658C2a
1 ABIN1952863 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN1953735 DB ELISA APC Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN1955016 DB ELISA Biotin Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN1958508 DB ELISA PE Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN1956178 DB ELISA FITC Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN1831921 DB ICC Mouse IgG2b Log in to see 658C2a
1 ABIN1957393 DB ELISA HRP Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN2599351 DB ELISA Rabbit IgG pSer332 Log in to see Polyclonal
1 ABIN2599349 DB ELISA WB Rabbit IgG His357 Log in to see Polyclonal
1 ABIN2256856 DB ELISA Rabbit IgG pSer332 Log in to see Polyclonal
1 ABIN2256860 DB ELISA FITC Rabbit IgG pSer332 Log in to see Polyclonal
1 ABIN1953307 DB ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
1 ABIN1954175 DB ELISA WB APC Rabbit IgG Log in to see Polyclonal
1 ABIN1957794 DB ELISA WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN2256858 DB ELISA APC Rabbit IgG pSer332 Log in to see Polyclonal
1 ABIN2256861 DB ELISA HRP Rabbit IgG pSer332 Log in to see Polyclonal

Similar anti-E2F1 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 39 Antibodies
ELISA 157 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies
Flow Cytometry (FACS) 13 Antibodies
Gel Shift (GS) 3 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 42 Antibodies
Immunohistochemistry (IHC) 78 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies
Immunoprecipitation (IP) 60 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 291 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope pHis357
(31), (30), (30), (27), (25), (15), (14), (14), (12), (11), (10), (10), (9), (9), (8), (8), (7), (6), (6), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(371), (102), (47), (10), (8), (6), (6), (5), (5), (4), (3), (3), (2), (1), (1)
Host Rabbit
(286), (73), (21), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(21), (19), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB)
(291), (166), (78), (60), (58), (55), (42), (38), (25), (13), (12), (3), (2), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling ProductDetails: References for anti-E2F1 antibody (ABIN650836) Images
Specificity This Phospho-E2F1-H357 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding H357 of human E2F1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This E2F1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding H357 of human E2F1.
Clone RB07916
Isotype Ig

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling ProductDetails: References for anti-E2F1 antibody (ABIN650836) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Synonyms: Retinoblastoma-associated protein 1, RBAP-1, PBR3, PRB-binding protein E2F-1, Retinoblastoma-binding protein 3, RBBP-3, Transcription factor E2F1, E2F-1,RBBP3, E2F1
Molecular Weight 46920 DA
Gene ID 1869
UniProt Q01094
Research Area Transcription Factors, Phospho-specific antibodies, Signaling
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling ProductDetails: References for anti-E2F1 antibody (ABIN650836) Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details ProductDetails: References for anti-E2F1 antibody (ABIN650836) Images back to top
Format Liquid
Concentration 0.26 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-E2F1 antibody (ABIN650836)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Background publications

Pulikkan, Dengler, Peramangalam, Peer Zada, Müller-Tidow, Bohlander, Tenen, Behre: "Cell-cycle regulator E2F1 and microRNA-223 comprise an autoregulatory negative feedback loop in acute myeloid leukemia." in: Blood, Vol. 115, Issue 9, pp. 1768-78, 2010

Paik, Wang, Liu, Lue, Lin: "Regulation of E2F1-induced apoptosis by the nucleolar protein RRP1B." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6348-63, 2010

Yang, Feng, Jiang, Wu, Li, Aau, Yu: "miR-449a and miR-449b are direct transcriptional targets of E2F1 and negatively regulate pRb-E2F1 activity through a feedback loop by targeting CDK6 and CDC25A." in: Genes & development, Vol. 23, Issue 20, pp. 2388-93, 2009

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling ProductDetails: References for anti-E2F1 antibody (ABIN650836) back to top
Supplier Images
Dot Blot (DB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (pHis357) (ABIN650836) Dot blot analysis of Phospho-hE2F1-H357 Pab (ABIN650836) on nitrocellulose membrane. ...