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|Application / Reactivity||Human|
|Dot Blot (DB)||41 Antibodies|
|Enzyme Immunoassay (EIA)||12 Antibodies|
|Flow Cytometry (FACS)||10 Antibodies|
|Gel Shift (GS)||2 Antibodies|
|Immunocytochemistry (ICC)||13 Antibodies|
|Immunofluorescence (IF)||32 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||39 Antibodies|
|Immunohistochemistry (IHC)||64 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||40 Antibodies|
|Immunoprecipitation (IP)||47 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||265 Antibodies|
|Antigen||E2F Transcription Factor 1 (E2F1) Antibodies|
|Conjugate||This E2F1 antibody is un-conjugated Alternatives|
Dot Blot (DB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-E2F1 AntibodyTarget Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN389869) Images
|Specificity||This Phospho-E2F1-S332 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S332 of human E2F1.|
|Purification||This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Immunogen||This E2F1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S332 of human E2F1.|
Target Details E2F1Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN389869) Images back to top
|Alternative Name||E2F1 (E2F1 Antibody Abstract)|
E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Synonyms: Transcription factor E2F1, E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, E2F1, RBBP3
|Molecular Weight||46920 DA|
|Research Area||Apoptosis/Necrosis, Cancer, Cell Cycle, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure|
|Pathways||p53 Signaling, Cell Division Cycle|
Application DetailsProduct Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN389869) Images back to top
|Application Notes||DB = 1:500|
|Restrictions||For Research Use only|
HandlingProduct Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN389869) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.|
|Expiry Date||6 months|
References for anti-E2F1 Antibody (ABIN389869)Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
ODonnell, Wentzel, Zeller et al.: "c-Myc-regulated microRNAs modulate E2F1 expression." in: Nature, Vol. 435, Issue 7043, pp. 839-43, 2005 (PubMed).
Joshi, Ordonez-Ercan, Dasgupta et al.: "Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors." in: Oncogene, Vol. 24, Issue 13, pp. 2204-17, 2005 (PubMed).
Wang, Hou, Mohapatra et al.: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005 (PubMed).
Chaussepied, Ginsberg: "Transcriptional regulation of AKT activation by E2F." in: Molecular cell, Vol. 16, Issue 5, pp. 831-7, 2004 (PubMed).
Saberwal, Lucas, Janssen et al.: "Increased levels and activity of E2F1 transcription factor in myelodysplastic bone marrow." in: International journal of hematology, Vol. 80, Issue 2, pp. 146-54, 2004 (PubMed).
ImagesProduct Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN389869) back to top