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anti-Human E2F1 antibody for Dot Blot

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Epitope
pSer332
31
30
29
27
25
15
14
14
12
11
10
9
9
9
8
8
7
7
6
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
376
100
43
9
8
6
5
4
4
3
3
3
3
2
2
Host
Rabbit
291
73
20
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB)
294
170
80
60
58
55
42
40
25
13
12
3
2
2
1
1
1
1
Options
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Catalog No. ABIN389869
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.08027 ABIN650836 DB Rabbit Ig pHis357 Log in to see Polyclonal 4
28.08027 ABIN2158619 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
28.08027 ABIN2158620 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
28.08027 ABIN3028630 DB ELISA Rabbit Ig Fraction pHis357 Log in to see Polyclonal
28.08027 ABIN3028631 DB ELISA Rabbit Ig Fraction pSer332 Log in to see Polyclonal
1 ABIN932458 DB ICC Mouse IgG2b Log in to see 658C2a
1 ABIN1831921 DB ICC Mouse IgG2b Log in to see 658C2a
1 ABIN1952863 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN1953735 DB ELISA APC Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN1955016 DB ELISA Biotin Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN1956178 DB ELISA FITC Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN1958508 DB ELISA PE Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN1957393 DB ELISA HRP Rabbit IgG Ser332 Log in to see Polyclonal
1 ABIN2599349 DB ELISA WB Rabbit IgG His357 Log in to see Polyclonal
1 ABIN2599351 DB ELISA Rabbit IgG pSer332 Log in to see Polyclonal
1 ABIN1953307 DB ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
1 ABIN1954175 DB ELISA WB APC Rabbit IgG Log in to see Polyclonal
1 ABIN1955417 DB ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN1956603 DB ELISA WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN1957794 DB ELISA WB HRP Rabbit IgG Log in to see Polyclonal

Top referenced anti-E2F1 antibody for Dot Blot

Similar anti-E2F1 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 41 Antibodies
ELISA 162 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies
Flow Cytometry (FACS) 13 Antibodies
Gel Shift (GS) 3 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 47 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 42 Antibodies
Immunohistochemistry (IHC) 80 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies
Immunoprecipitation (IP) 60 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 294 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope pSer332
(31), (30), (29), (27), (25), (15), (14), (14), (12), (11), (10), (9), (9), (9), (8), (8), (7), (7), (6), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(376), (100), (43), (9), (8), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2)
Host Rabbit
(291), (73), (20), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB)
(294), (170), (80), (60), (58), (55), (42), (40), (25), (13), (12), (3), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN389869) Images
Specificity This Phospho-E2F1-S332 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S332 of human E2F1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This E2F1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S332 of human E2F1.
Clone RB8110
Isotype Ig

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN389869) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Synonyms: Transcription factor E2F1, E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, E2F1, RBBP3
Molecular Weight 46920 DA
Gene ID 1869
UniProt Q01094
Research Area Apoptosis/Necrosis, Cancer, Cell Cycle, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN389869) Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN389869) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-E2F1 Antibody (ABIN389869)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Background publications

ODonnell, Wentzel, Zeller, Dang, Mendell: "c-Myc-regulated microRNAs modulate E2F1 expression." in: Nature, Vol. 435, Issue 7043, pp. 839-43, 2005

Wang, Hou, Mohapatra, Ma, Cress, Pledger, Chen: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005

Joshi, Ordonez-Ercan, Dasgupta, Chellappan: "Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors." in: Oncogene, Vol. 24, Issue 13, pp. 2204-17, 2005

Saberwal, Lucas, Janssen, Deobhakta, Hu, Galili, Raza, Mundle: "Increased levels and activity of E2F1 transcription factor in myelodysplastic bone marrow." in: International journal of hematology, Vol. 80, Issue 2, pp. 146-54, 2004

Chaussepied, Ginsberg: "Transcriptional regulation of AKT activation by E2F." in: Molecular cell, Vol. 16, Issue 5, pp. 831-7, 2004

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN389869) back to top
Supplier Images
Dot Blot (DB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (pSer332) (ABIN389869) Dot blot analysis of anti-E2F1-pS332 Pab (ABIN389869) on nitrocellulose membrane. 50...