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anti-Human E2F1 antibody for Immunohistochemistry

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • ATE2FB
  • CG6376
  • dE2f
  • dE2F
  • dE2F1
  • dE2f1
  • de2f1
  • def21
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • drosE2F1
  • DRTF1/E2F
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • e2f
  • E2F
  • E2F-1
  • e2f1
  • E2f1
  • E2F1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • Evar(3)164
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • mKIAA4009
  • RBAP1
  • RBBP3
  • RBP3
  • xE2F
Epitope
AA 69-223
31
28
27
25
25
14
14
13
10
10
9
9
9
8
8
7
7
6
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
356
73
29
9
4
3
3
3
1
1
1
Host
Mouse
274
71
19
1
Clonality
Monoclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
279
161
79
60
55
54
41
36
27
12
11
2
2
2
1
1
Supplier
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Catalog No. ABIN969516
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22099273 ABIN129619 IHC ELISA WB Rabbit IgG AA 360-369, pSer364 Log in to see Polyclonal
0.22099273 ABIN2704741 IHC WB Rabbit C-Term Log in to see Polyclonal
0.22099273 ABIN2470299 IHC ELISA WB Rabbit pSer364 Log in to see Polyclonal
0.22099273 ABIN2160780 IHC WB Rabbit Ig Fraction pSer337 Log in to see Polyclonal
0.22099273 ABIN1845545 FACS IHC ELISA WB Mouse IgG1 AA 69-223 Log in to see
0.22099273 ABIN4306767 ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8G9
0.0011390361 ABIN153153 ICC IF IP IHC WB Mouse IgG1 kappa Log in to see 1-80E11 2
0.0011390361 ABIN1491636 FACS IHC ELISA WB Mouse IgG1 Log in to see 8G9
0.0011390361 ABIN269710 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa Log in to see 16G7 1
0.0011390361 ABIN1872398 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011390361 ABIN4880694 ELISA FACS IHC WB Mouse IgG1 AA 69-223 Log in to see 8G9
0.0011390361 ABIN958104 IHC WB Sheep IgG AA 264-437 Log in to see Polyclonal
0.0011390361 ABIN1956180 IHC ELISA WB FITC Rabbit IgG Ser337 Log in to see Polyclonal
0.0011390361 ABIN1955018 IHC ELISA WB Biotin Rabbit IgG Ser337 Log in to see Polyclonal
0.0011390361 ABIN791204 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011390361 ABIN1958510 IHC ELISA WB PE Rabbit IgG Ser337 Log in to see Polyclonal
0.0011390361 ABIN1952865 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Ser337 Log in to see Polyclonal
0.0011390361 ABIN1953737 IHC ELISA WB APC Rabbit IgG Ser337 Log in to see Polyclonal
0.0011390361 ABIN3022840 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011390361 ABIN4306768 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1

Top referenced anti-E2F1 antibody for Immunohistochemistry

Similar anti-E2F1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 24 Antibodies 154 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 12 Antibodies
Flow Cytometry (FACS) 4 Antibodies 12 Antibodies
Immunocytochemistry (ICC) 17 Antibodies 27 Antibodies
Immunofluorescence (IF) 20 Antibodies 46 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 3 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Western Blotting (WB) 71 Antibodies
Dot Blot (DB) 41 Antibodies
Gel Shift (GS) 2 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope AA 69-223
(31), (28), (27), (25), (25), (14), (14), (13), (10), (10), (9), (9), (9), (8), (8), (7), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(356), (73), (29), (9), (4), (3), (3), (3), (1), (1), (1)
Host Mouse
(274), (71), (19), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(279), (161), (79), (60), (55), (54), (41), (36), (27), (12), (11), (2), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN969516) Images
Purification Purified
Immunogen Purified recombinant fragment of human E2F1 (AA 69-223) expressed in E. coli.
Clone 8G9
Isotype IgG1

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN969516) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Molecular Weight 46.9 kDa
Gene ID 1869
HGNC 1869
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN969516) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN969516) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-E2F1 Antibody (ABIN969516)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Product cited in:

Okazaki, Matsunaga, Okazaki, Utoguchi, Suzuki, Maruyama, Koyanagi, Ohdo: "Circadian rhythm of transferrin receptor 1 gene expression controlled by c-Myc in colon cancer-bearing mice." in: Cancer research, Vol. 70, Issue 15, pp. 6238-46, 2010

Gurtner, Fuschi, Martelli, Manni, Artuso, Simonte, Ambrosino, Antonini, Folgiero, Falcioni, Sacchi, Piaggio: "Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation." in: Cancer research, Vol. 70, Issue 23, pp. 9711-20, 2010

Locatelli, Bosotti, Ciomei, Brasca, Calogero, Mercurio, Fiorentini, Bertolotti, Scacheri, Scaburri, Galvani, Pesenti, De Baere, Soria, Lazar, Isacchi: "Transcriptional analysis of an E2F gene signature as a biomarker of activity of the cyclin-dependent kinase inhibitor PHA-793887 in tumor and skin biopsies from a phase I clinical study." in: Molecular cancer therapeutics, Vol. 9, Issue 5, pp. 1265-73, 2010

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN969516) back to top
Supplier Images
ELISA image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 2: Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and neg...
Immunohistochemistry (IHC) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 3: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues...
Immunohistochemistry (IHC) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 5: Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), a...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 6: Western blot analysis using E2F1 mAb against human E2F1 recombinant protein...