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anti-Mouse (Murine) E2F1 antibody for Immunohistochemistry

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Epitope
AA 69-223
30
30
30
27
25
15
14
14
12
11
10
9
9
9
8
8
6
6
6
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
365
97
43
9
8
6
5
4
4
3
3
3
2
2
2
Host
Mouse
281
72
20
1
Clonality
Monoclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
284
161
77
60
58
54
42
39
25
12
12
3
2
2
1
1
1
Options
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Catalog No. ABIN969516
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.142917 ABIN2704741 IHC WB Rabbit C-Term Log in to see Polyclonal
9.142917 ABIN4306767 CyTOF ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8G9
9.142917 ABIN1845545 FACS IHC ELISA WB Mouse IgG1 AA 69-223 Log in to see
1 ABIN269710 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 16G7 1
1 ABIN3184378 ELISA IHC WB Rabbit IgG Ser310 Log in to see Polyclonal
1 ABIN2736667 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4880694 ELISA FACS IHC WB Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN3022840 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4306766 IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN571306 IHC ELISA WB Rabbit IgG Internal Region, Lys120 Log in to see Polyclonal
1 ABIN408534 IHC (p) IHC ELISA WB Rabbit pThr433 Log in to see Polyclonal
1 ABIN1585777 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN4306779 ICC IF IHC IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 Log in to see 16G7
1 ABIN4306772 ICC IF IHC IHC (p) IP WB PerCP Mouse IgG1 Log in to see 16G7
1 ABIN4306769 ICC IF IHC IHC (p) IP WB DyLight 350 Mouse IgG1 Log in to see 16G7
1 ABIN4306770 ICC IF IHC IHC (p) IP WB DyLight 405 Mouse IgG1 Log in to see 16G7
1 ABIN4306775 ICC IF IHC IHC (p) IP WB DyLight 488 Mouse IgG1 Log in to see 16G7
1 ABIN4306777 ICC IF IHC IHC (p) IP WB DyLight 680 Mouse IgG1 Log in to see 16G7
1 ABIN4306778 ICC IF IHC IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 Log in to see 16G7
1 ABIN4306780 ICC IF IHC IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 Log in to see 16G7

Top referenced anti-E2F1 antibody for Immunohistochemistry

Similar anti-E2F1 Antibodies

Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies 1 Antibodies
ELISA 33 Antibodies 154 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 12 Antibodies
Flow Cytometry (FACS) 5 Antibodies 13 Antibodies
Gel Shift (GS) 3 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 3 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 93 Antibodies
Dot Blot (DB) 39 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope AA 69-223
(30), (30), (30), (27), (25), (15), (14), (14), (12), (11), (10), (9), (9), (9), (8), (8), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(365), (97), (43), (9), (8), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2)
Host Mouse
(281), (72), (20), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(284), (161), (77), (60), (58), (54), (42), (39), (25), (12), (12), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN969516) Images
Purification Purified
Immunogen Purified recombinant fragment of human E2F1 (AA 69-223) expressed in E. coli.
Clone 8G9
Isotype IgG1

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN969516) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Molecular Weight 46.9 kDa
Gene ID 1869
HGNC 1869
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN969516) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN969516) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-E2F1 Antibody (ABIN969516)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Product cited in:

Okazaki, Matsunaga, Okazaki, Utoguchi, Suzuki, Maruyama, Koyanagi, Ohdo: "Circadian rhythm of transferrin receptor 1 gene expression controlled by c-Myc in colon cancer-bearing mice." in: Cancer research, Vol. 70, Issue 15, pp. 6238-46, 2010

Gurtner, Fuschi, Martelli, Manni, Artuso, Simonte, Ambrosino, Antonini, Folgiero, Falcioni, Sacchi, Piaggio: "Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation." in: Cancer research, Vol. 70, Issue 23, pp. 9711-20, 2010

Locatelli, Bosotti, Ciomei, Brasca, Calogero, Mercurio, Fiorentini, Bertolotti, Scacheri, Scaburri, Galvani, Pesenti, De Baere, Soria, Lazar, Isacchi: "Transcriptional analysis of an E2F gene signature as a biomarker of activity of the cyclin-dependent kinase inhibitor PHA-793887 in tumor and skin biopsies from a phase I clinical study." in: Molecular cancer therapeutics, Vol. 9, Issue 5, pp. 1265-73, 2010

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN969516) back to top
Supplier Images
ELISA image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 2: Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and neg...
Immunohistochemistry (IHC) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 3: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues...
Immunohistochemistry (IHC) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 5: Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), a...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 6: Western blot analysis using E2F1 mAb against human E2F1 recombinant protein...