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anti-Mouse (Murine) E2F1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • ATE2FB
  • CG6376
  • dE2f
  • dE2F
  • dE2F1
  • dE2f1
  • de2f1
  • def21
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • drosE2F1
  • DRTF1/E2F
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • e2f
  • E2F
  • E2F-1
  • e2f1
  • E2f1
  • E2F1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • Evar(3)164
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • mKIAA4009
  • RBAP1
  • RBBP3
  • RBP3
  • xE2F
Reactivity
Human, Mouse (Murine)
350
71
29
9
4
3
3
3
1
1
1
Host
Mouse
274
65
19
1
Clonality
Monoclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
273
159
76
53
53
49
41
36
23
12
11
2
2
1
1
1
Supplier
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Catalog No. ABIN115204
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008985354 ABIN271885 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008985354 ABIN115203 IF IHC (p) IP WB Mouse IgG2a Log in to see KH95
0.0008985354 ABIN670686 IF (p) IHC (p) WB Rabbit IgG AA 105-135 Log in to see Polyclonal
0.0008985354 ABIN408534 IHC (p) IHC ELISA WB Rabbit IgG Thr433 Log in to see Polyclonal
0.0008985354 ABIN1386153 IF (p) IHC (p) WB Rabbit IgG pSer375 Log in to see Polyclonal
0.0008985354 ABIN1386489 IF (p) IHC (p) WB Rabbit IgG pThr433 Log in to see Polyclonal
0.0008985354 ABIN4306767 ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8G9
0.0008985354 ABIN4306769 ICC IF IHC IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306770 ICC IF IHC IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306772 ICC IF IHC IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306773 ICC IF IHC IHC (p) IP WB Allophycocyanin Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306775 ICC IF IHC IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306777 ICC IF IHC IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306778 ICC IF IHC IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306779 ICC IF IHC IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306780 ICC IF IHC IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306781 ICC IF IHC IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306771 ICC IF IHC IHC (p) IP WB PE Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306774 ICC IF IHC IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see 16G7
0.0008985354 ABIN4306776 ICC IF IHC IHC (p) IP WB DyLight 405LS Mouse IgG1 kappa Log in to see 16G7

Top referenced anti-E2F1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-E2F1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 23 Antibodies 153 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 12 Antibodies
Flow Cytometry (FACS) 3 Antibodies 11 Antibodies
Immunocytochemistry (ICC) 15 Antibodies 27 Antibodies
Immunofluorescence (IF) 18 Antibodies 46 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 3 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Western Blotting (WB) 70 Antibodies
Dot Blot (DB) 41 Antibodies
Gel Shift (GS) 2 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Reactivity Human, Mouse (Murine)
(350), (71), (29), (9), (4), (3), (3), (3), (1), (1), (1)
Host Mouse
(274), (65), (19), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(273), (159), (76), (53), (53), (49), (41), (36), (23), (12), (11), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN115204) Images
Specificity This antibody reacts with a protein of 60 kDa.
Cellular Localization: Nuclear.
Purification Purified
Immunogen BALB/C mice were immunized with a recombinant Human E2F-1 protein.
Clone KH95
Isotype IgG2a

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN115204) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background E2Fs are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
Gene ID 1869
NCBI Accession NP_005216
UniProt Q01094
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN115204) Images back to top
Application Notes Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil, Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN115204) Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-E2F1 Antibody (ABIN115204)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Background publications

Helin, Harlow: "Heterodimerization of the transcription factors E2F-1 and DP-1 is required for binding to the adenovirus E4 (ORF6/7) protein." in: Journal of virology, Vol. 68, Issue 8, pp. 5027-35, 1994

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN115204) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-E2F1 antibody (E2F Transcription Factor 1) (ABIN115204) Formalin fixed paraffin embedded human tonsil stained with E2F-1 Transcription Factor...