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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||23 Antibodies||153 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||12 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies||11 Antibodies|
|Immunocytochemistry (ICC)||15 Antibodies||27 Antibodies|
|Immunofluorescence (IF)||18 Antibodies||46 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||3 Antibodies|
|Immunohistochemistry (IHC)||32 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||22 Antibodies|
|Immunoprecipitation (IP)||17 Antibodies|
|Western Blotting (WB)||70 Antibodies|
|Dot Blot (DB)||41 Antibodies|
|Gel Shift (GS)||2 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Antigen||E2F Transcription Factor 1 (E2F1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This E2F1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-E2F1 AntibodyTarget Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN115204) Images
This antibody reacts with a protein of 60 kDa.
Cellular Localization: Nuclear.
|Immunogen||BALB/C mice were immunized with a recombinant Human E2F-1 protein.|
Target Details E2F1Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN115204) Images back to top
|Alternative Name||E2F1 (E2F1 Antibody Abstract)|
|Background||E2Fs are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1|
|Research Area||Cancer, Transcription Factors|
|Pathways||p53 Signaling, Cell Division Cycle|
Application DetailsProduct Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN115204) Images back to top
Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil, Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN115204) Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-E2F1 Antibody (ABIN115204)Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Helin, Harlow: "Heterodimerization of the transcription factors E2F-1 and DP-1 is required for binding to the adenovirus E4 (ORF6/7) protein." in: Journal of virology, Vol. 68, Issue 8, pp. 5027-35, 1994
ImagesProduct Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN115204) back to top