We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This GADD45A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-GADD45A AntibodyTarget Details GADD45A Application Details Handling References for anti-GADD45A antibody (ABIN357951) Images
|Specificity||This antibody detects GADD45A (N-term).|
|Purification||Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GADD45A.|
Target Details GADD45AProduct Details anti-GADD45A Antibody Application Details Handling References for anti-GADD45A antibody (ABIN357951) Images back to top
|Alternative Name||GADD45A (GADD45A Antibody Abstract)|
|Background||GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.Synonyms: DDIT-1, DDIT1, DNA damage-inducible transcript 1 protein, GADD45, Growth arrest and DNA damage-inducible protein GADD45 alpha|
|Molecular Weight||18205 Da predicted from amino acid sequence. Theoretical pI: 4.36|
|Gene ID||1647, 9606|
|Research Area||Chromatin and Nuclear Signaling, DNA/RNA|
|Pathways||p53 Signaling, Cell Division Cycle|
Application DetailsProduct Details anti-GADD45A Antibody Target Details GADD45A Handling References for anti-GADD45A antibody (ABIN357951) Images back to top
ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GADD45A Antibody Target Details GADD45A Application Details References for anti-GADD45A antibody (ABIN357951) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-GADD45A antibody (ABIN357951)Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling Images back to top
Al-Romaih, Sadikovic, Yoshimoto, Wang, Zielenska, Squire: "Decitabine-induced demethylation of 5' CpG island in GADD45A leads to apoptosis in osteosarcoma cells." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 5, pp. 471-80, 2008
Li, Morales, Hwang, Wagner, Boothman: "DNA mismatch repair-dependent activation of c-Abl/p73alpha/GADD45alpha-mediated apoptosis." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21394-403, 2008
ImagesProduct Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling References for anti-GADD45A antibody (ABIN357951) back to top