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anti-Mouse (Murine) GADD45A antibody for Enzyme Immunoassay

Recommended GADD45A Antibody (supplied by: Log in to see )

Antigen
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies
  • AA545191
  • ddit1
  • Ddit1
  • DDIT1
  • Gadd45
  • GADD45
  • gadd45
  • gadd45a
  • GADD45A
  • MGC53491
  • zgc:91795
Epitope
N-Term
14
13
10
6
4
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
76
28
28
Host
Rabbit
64
13
Clonality
Polyclonal
Conjugate
This GADD45A antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
55
19
11
11
6
4
1
1
1
1
Supplier
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Catalog No. ABIN357951
$ 390.50
Plus shipping costs $45.00

Top referenced anti-GADD45A antibody for Enzyme Immunoassay

Similar anti-GADD45A Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 6 Antibodies 19 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 4 Antibodies
Western Blotting (WB) 13 Antibodies 56 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies
Epitope N-Term
(14), (13), (10), (6), (4), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(76), (28), (28)
Host Rabbit
(64), (13)
Clonality
Polyclonal
Conjugate This GADD45A antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(55), (19), (11), (11), (6), (4), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-GADD45A Antibody

Target Details GADD45A Application Details Handling References for anti-GADD45A Antibody (ABIN357951) Images
Specificity This antibody detects GADD45A (N-term).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GADD45A.
Isotype Ig

Target Details GADD45A

Product Details anti-GADD45A Antibody Application Details Handling References for anti-GADD45A Antibody (ABIN357951) Images back to top
Antigen
Alternative Name GADD45A (GADD45A Antibody Abstract)
Background GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.Synonyms: DDIT-1, DDIT1, DNA damage-inducible transcript 1 protein, GADD45, Growth arrest and DNA damage-inducible protein GADD45 alpha
Molecular Weight 18205 Da predicted from amino acid sequence. Theoretical pI: 4.36
Gene ID 1647, 9606
UniProt P24522
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-GADD45A Antibody Target Details GADD45A Handling References for anti-GADD45A Antibody (ABIN357951) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details References for anti-GADD45A Antibody (ABIN357951) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-GADD45A Antibody (ABIN357951)

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling Images back to top
Background publications

Al-Romaih, Sadikovic, Yoshimoto, Wang, Zielenska, Squire: "Decitabine-induced demethylation of 5' CpG island in GADD45A leads to apoptosis in osteosarcoma cells." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 5, pp. 471-80, 2008

Li, Morales, Hwang, Wagner, Boothman: "DNA mismatch repair-dependent activation of c-Abl/p73alpha/GADD45alpha-mediated apoptosis." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21394-403, 2008

Images

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling References for anti-GADD45A Antibody (ABIN357951) back to top
Supplier Images
 image for anti-GADD45A antibody (Growth Arrest and DNA-Damage-Inducible, alpha) (N-Term) (ABIN357951) anti-Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (N-Term) antibody