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anti-Cow (Bovine) HMGB1 antibody for Western Blotting

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • hmgb1
  • amphoterin
  • hmg-1
  • hmg1
  • hmg3
  • sbp-1
  • HMGB1
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • p30
  • Ac2-008
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • Hmgb1
  • high-mobility group box 1
  • high mobility group box 1
  • high mobility group protein B1-like
  • high-mobility group box 1a
  • HMG-box transcription factor 1
  • hmgb1
  • HMGB1
  • Hmgb1
  • LOC100392312
  • LOC100754755
  • LOC100359149
  • hmgb1a
  • Hbp1
Epitope
N-Term
69
53
38
38
38
20
19
19
18
16
15
13
12
12
11
11
7
5
5
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
437
208
140
50
38
29
14
13
6
5
3
1
Host
Rabbit
241
205
4
4
2
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
27
20
18
16
8
8
8
7
7
7
7
7
7
6
6
5
5
5
5
3
2
2
1
1
1
1
1
1
1
Application
Western Blotting (WB)
366
189
129
128
105
83
45
19
13
12
6
5
3
2
1
1
1
1
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Catalog No. ABIN2780399
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0246496 ABIN2780400 WB Rabbit Middle Region Log in to see Polyclonal 1
3.0246496 ABIN2776383 WB Rabbit Middle Region Log in to see Polyclonal 1
3.0246496 ABIN250703 ELISA FACS ICC IF IHC IHC (p) SimWes WB Rabbit AA 100-200, Internal Region Log in to see Polyclonal 12
3.0246496 ABIN2957615 WB Rabbit N-Term, acLys12 Log in to see Polyclonal
3.0246496 ABIN2957616 IHC WB Rabbit N-Term Log in to see Polyclonal
3.0246496 ABIN2506663 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.0246496 ABIN2957614 IC IF IHC WB Rabbit Log in to see Polyclonal
3.0246496 ABIN4319335 CyTOF FACS ICC IF SimWes WB Mouse IgG2b Log in to see 1F3
3.0246496 ABIN1580427 ICC IF IHC WB Mouse IgG2b Log in to see 1F3
1 ABIN1107585 WB Mouse IgG1 Log in to see FBH7 3
1 ABIN1735426 ICC FACS IF IHC WB Rabbit AA 100-200 Log in to see Polyclonal
1 ABIN407016 ELISA IHC IHC (p) WB HRP Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN407013 ELISA ICC IF IHC IHC (p) WB Biotin Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN407015 ELISA FACS ICC IF IHC IHC (p) WB DyLight 488 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319334 CyTOF FACS ICC IF SimWes WB Mouse IgG2b Log in to see 1F3
1 ABIN1735500 IHC (p) WB DyLight 650 Rabbit IgG AA 100-200 Log in to see Polyclonal
1 ABIN4319385 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319384 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319322 ELISA FACS ICC IF IHC IHC (p) WB DyLight 650 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319321 ELISA FACS ICC IF IHC IHC (p) WB DyLight 550 Rabbit AA 100-200, Internal Region Log in to see Polyclonal

Top referenced anti-HMGB1 antibody for Western Blotting

Similar anti-HMGB1 Antibodies

Application / Reactivity Cow (Bovine) Human Mouse (Murine) Pig (Porcine) Rat (Rattus) Dog (Canine) Chicken Xenopus laevis
Cytometry by Time of Flight (CyTOF) 2 Antibodies 5 Antibodies 5 Antibodies 2 Antibodies 2 Antibodies
Simple Western (SimWes) 3 Antibodies 12 Antibodies 8 Antibodies 2 Antibodies 3 Antibodies 1 Antibodies
Western Blotting (WB) 354 Antibodies 32 Antibodies 25 Antibodies 42 Antibodies 165 Antibodies 104 Antibodies 4 Antibodies 1 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope N-Term
(69), (53), (38), (38), (38), (20), (19), (19), (18), (16), (15), (13), (12), (12), (11), (11), (7), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
(437), (208), (140), (50), (38), (29), (14), (13), (6), (5), (3), (1)
Host Rabbit
(241), (205), (4), (4), (2), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(27), (20), (18), (16), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (5), (5), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(366), (189), (129), (128), (105), (83), (45), (19), (13), (12), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2780399) Images
Blocking Peptide Blocking peptide for this product available: ABIN978051
Sequence GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against HMGB1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HMGB1

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 Antibody (ABIN2780399) Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background HMGB1 belongs to the HMGB family. It contains 2 HMG box DNA-binding domains. The protein binds preferentially single-stranded DNA and unwinds double stranded DNA. The oxidized HMGB1 may accumulate even in cells under oxidative stress.
Molecular Weight 25 kDa
Gene ID 3146
NCBI Accession NM_002128, NP_002119
UniProt P09429
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 Antibody (ABIN2780399) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 215 AA

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 Antibody (ABIN2780399) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HMGB1 Antibody (ABIN2780399)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Product cited in:

Straino, Di Carlo, Mangoni, De Mori, Guerra, Maurelli, Panacchia, Di Giacomo, Palumbo, Di Campli, Uccioli, Biglioli, Bianchi, Capogrossi, Germani: "High-mobility group box 1 protein in human and murine skin: involvement in wound healing." in: The Journal of investigative dermatology, Vol. 128, Issue 6, pp. 1545-53, 2008

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2780399) back to top
Supplier Images
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (N-Term) (ABIN2780399) WB Suggested Anti-HMGB1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: