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anti-Cow (Bovine) HMGB1 antibody for Western Blotting

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • Ac2-008
  • amphoterin
  • DEF
  • hmg-1
  • HMG-1
  • hmg1
  • HMG1
  • Hmg1
  • HMG3
  • hmg3
  • Hmgb1
  • HMGB1
  • hmgb1
  • ik:tdsubc_1a5
  • p30
  • sbp-1
  • SBP-1
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
Epitope
N-Term
34
27
18
16
13
12
12
11
9
7
7
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
295
112
101
18
18
16
6
5
3
1
1
Host
Rabbit
212
94
4
4
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
20
15
11
9
8
7
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
250
158
75
60
53
27
14
12
11
6
6
2
1
1
1
1
Supplier
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Catalog No. ABIN2780399
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12226487 ABIN2776383 WB Rabbit Middle Region Log in to see Polyclonal 1
0.12226487 ABIN2780400 WB Rabbit Middle Region Log in to see Polyclonal 1
0.12226487 ABIN250705 ICC FACS IF IHC (p) IHC WB Rabbit AA 100-200 Log in to see Polyclonal
0.12226487 ABIN2506663 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.12226487 ABIN1580427 ICC IF IHC WB Mouse IgG2b Log in to see 1F3
0.0011728628 ABIN1107585 WB Mouse IgG1 Log in to see FBH7 3
0.0011728628 ABIN1735426 ICC FACS IF IHC WB Rabbit Internal Region, AA 100-200 Log in to see Polyclonal
0.0011728628 ABIN2957616 IHC WB Rabbit N-Term Log in to see Polyclonal
0.0011728628 ABIN2957615 WB Rabbit N-Term, acLys12 Log in to see Polyclonal
0.0011728628 ABIN2957614 IC IF IHC WB Rabbit Log in to see Polyclonal
0.0011728628 ABIN407015 IHC (p) IHC WB DyLight 488 Rabbit AA 100-200 Log in to see Polyclonal
0.0011728628 ABIN407013 IHC (p) IHC WB Biotin Rabbit AA 100-200 Log in to see Polyclonal
0.0011728628 ABIN407016 IHC (p) IHC WB HRP Rabbit AA 100-200 Log in to see Polyclonal
0.0011728628 ABIN1735500 IHC (p) WB DyLight 650 Rabbit IgG AA 100-200 Log in to see Polyclonal
0.0011728628 ABIN1735499 IHC (p) WB Biotin Rabbit IgG AA 100-200 Log in to see Polyclonal
0.0011728628 ABIN1735501 IHC (p) WB DyLight 488 Rabbit IgG AA 100-200 Log in to see Polyclonal
0.0011728628 ABIN1735502 IHC (p) WB HRP Rabbit IgG AA 100-200 Log in to see Polyclonal
0.0011728628 ABIN1735503 IHC (p) WB DyLight 550 Rabbit IgG AA 100-200 Log in to see Polyclonal
0.0011728628 ABIN1951825 ICC IF WB Mouse IgG2b Log in to see

Top referenced anti-HMGB1 antibody for Western Blotting

Similar anti-HMGB1 Antibodies

Application / Reactivity Human Pig (Porcine) Dog (Canine) Cow (Bovine) Mouse (Murine) Rat (Rattus) Chicken Xenopus laevis
Western Blotting (WB) 244 Antibodies 17 Antibodies 17 Antibodies 20 Antibodies 86 Antibodies 76 Antibodies 4 Antibodies 1 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 12 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 62 Antibodies 10 Antibodies 22 Antibodies 5 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope N-Term
(34), (27), (18), (16), (13), (12), (12), (11), (9), (7), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
(295), (112), (101), (18), (18), (16), (6), (5), (3), (1), (1)
Host Rabbit
(212), (94), (4), (4), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(20), (15), (11), (9), (8), (7), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(250), (158), (75), (60), (53), (27), (14), (12), (11), (6), (6), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2780399) Images
Blocking Peptide Blocking peptide for this product available: ABIN978051
Sequence GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against HMGB1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HMGB1

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 Antibody (ABIN2780399) Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background HMGB1 belongs to the HMGB family. It contains 2 HMG box DNA-binding domains. The protein binds preferentially single-stranded DNA and unwinds double stranded DNA. The oxidized HMGB1 may accumulate even in cells under oxidative stress.
Molecular Weight 25 kDa
Gene ID 3146
NCBI Accession NP_002119, NM_002128
UniProt P09429
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 Antibody (ABIN2780399) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 215 AA

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 Antibody (ABIN2780399) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HMGB1 Antibody (ABIN2780399)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Product cited in:

Straino, Di Carlo, Mangoni, De Mori, Guerra, Maurelli, Panacchia, Di Giacomo, Palumbo, Di Campli, Uccioli, Biglioli, Bianchi, Capogrossi, Germani: "High-mobility group box 1 protein in human and murine skin: involvement in wound healing." in: The Journal of investigative dermatology, Vol. 128, Issue 6, pp. 1545-53, 2008

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2780399) back to top
Supplier Images
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (N-Term) (ABIN2780399) WB Suggested Anti-HMGB1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: