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|Application / Reactivity||Human|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||5 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||109 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||40 Antibodies|
|Immunofluorescence (IF)||79 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||116 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||131 Antibodies|
|Immunoprecipitation (IP)||17 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Simple Western (SimWes)||12 Antibodies|
|Western Blotting (WB)||359 Antibodies|
|Antigen||High-Mobility Group Box 1 (HMGB1) Antibodies|
|Epitope||AA 1-215, N-Term Alternatives|
|Conjugate||This HMGB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-HMGB1 AntibodyTarget Details HMGB1 Application Details Handling Images
|Specificity||The antibody recognizes human HMGB1. Other species not tested.|
|Characteristics||Synonyms: High mobility group protein 1, HMG1, HMG-1, High mobility group protein B1, HMGB-1,Amphoterin|
|Purification||Protein-G affinity chromatography|
|Immunogen||Recombinant human HMGB1 (1-215aa) purified from High Five (Trichoplusia ni) insect cells.|
Target Details HMGB1Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
|Alternative Name||HMGB1 (HMGB1 Antibody Abstract)|
|Background||High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.Synonyms: Amphoterin, HMG-1, HMG1, HMGB-1, High mobility group protein 1, High mobility group protein B1|
|Research Area||Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology|
|Pathways||p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration|
Application DetailsProduct Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
ELISA. Western blot (1: 500 - 1: 2,000), recommended starting dilution is 1: 1,000. Immunohistochemistry (1: 100 - 1: 300), recommended starting dilution is 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
|Buffer||PBS, pH 7.4, containing 0.09 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top