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anti-Human HMGB1 antibody for Enzyme Immunoassay

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • Ac2-008
  • amphoterin
  • DEF
  • hmg-1
  • HMG-1
  • hmg1
  • HMG1
  • Hmg1
  • HMG3
  • hmg3
  • Hmgb1
  • HMGB1
  • hmgb1
  • ik:tdsubc_1a5
  • p30
  • sbp-1
  • SBP-1
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
Epitope
AA 1-215, N-Term
33
27
18
16
13
12
12
11
9
7
7
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
295
113
102
19
19
17
6
6
3
1
1
1
Host
Mouse
213
93
4
4
2
Clonality
Monoclonal
Conjugate
This HMGB1 antibody is un-conjugated
20
15
11
9
8
7
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
250
158
75
59
53
27
14
12
11
6
5
2
1
1
1
1
Supplier
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Catalog No. ABIN356226
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN614615 EIA IF IHC (p) WB Mouse IgG2a AA 1-216 Log in to see 1D5
0.0008833851 ABIN1107586 EIA IHC (fro) WB Rabbit N-Term, meLys42 Log in to see Polyclonal 1
0.0008833851 ABIN614617 EIA IF IHC (p) WB Mouse IgG2a AA 1-91 Log in to see 2F6
0.0008833851 ABIN614616 EIA IF IHC (p) WB Mouse IgG2a AA 1-216 Log in to see 1B11
0.0008833851 ABIN1107583 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-HMGB1 antibody for Enzyme Immunoassay

Similar anti-HMGB1 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
ELISA 149 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 27 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 77 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 62 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 244 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope AA 1-215, N-Term
(33), (27), (18), (16), (13), (12), (12), (11), (9), (7), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(295), (113), (102), (19), (19), (17), (6), (6), (3), (1), (1), (1)
Host Mouse
(213), (93), (4), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HMGB1 antibody is un-conjugated
(20), (15), (11), (9), (8), (7), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(250), (158), (75), (59), (53), (27), (14), (12), (11), (6), (5), (2), (1), (1), (1), (1)
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling Images
Specificity The antibody recognizes human HMGB1. Other species not tested.
Characteristics Synonyms: High mobility group protein 1, HMG1, HMG-1, High mobility group protein B1, HMGB-1,Amphoterin
Purification Protein-G affinity chromatography
Immunogen Recombinant human HMGB1 (1-215aa) purified from High Five (Trichoplusia ni) insect cells.
Clone J2E1
Isotype IgG2b

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.Synonyms: Amphoterin, HMG-1, HMG1, HMGB-1, High mobility group protein 1, High mobility group protein B1
Gene ID 3146
UniProt P09429
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
Application Notes ELISA. Western blot (1: 500 - 1: 2,000), recommended starting dilution is 1: 1,000. Immunohistochemistry (1: 100 - 1: 300), recommended starting dilution is 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 1-215) (ABIN356226) anti-High-Mobility Group Box 1 (HMGB1) (AA 1-215), (N-Term) antibody
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 1-215) (ABIN356226) anti-High-Mobility Group Box 1 (HMGB1) (AA 1-215), (N-Term) antibody (Image 2)