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anti-Human HMGB1 antibody for Immunocytochemistry

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • Ac2-008
  • amphoterin
  • DEF
  • hmg-1
  • HMG-1
  • hmg1
  • HMG1
  • Hmg1
  • HMG3
  • hmg3
  • Hmgb1
  • HMGB1
  • hmgb1
  • ik:tdsubc_1a5
  • p30
  • sbp-1
  • SBP-1
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
Epitope
Center
65
52
40
39
39
20
19
19
18
17
13
12
12
11
10
8
6
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
400
187
118
34
30
22
14
13
6
6
3
1
1
Host
Rabbit
232
179
4
4
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
25
17
17
14
8
7
7
6
6
6
6
6
6
5
4
4
4
4
4
4
3
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
348
178
132
90
84
62
23
12
12
11
6
6
2
1
1
1
1
Supplier
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Catalog No. ABIN2854997
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN2506663 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN250705 FACS ICC IF IHC IHC (p) SimWes WB Rabbit AA 100-200, Internal Region Log in to see Polyclonal 12
0.0009962841 ABIN1735426 ICC FACS IF IHC WB Rabbit Internal Region, AA 100-200 Log in to see Polyclonal
0.0009962841 ABIN1859178 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 9-163 Log in to see Polyclonal
0.0009962841 ABIN1078145 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-215 Log in to see Polyclonal
0.0009962841 ABIN448273 ICC FACS IF IHC (p) IHC WB Rabbit IgG Log in to see EPR3507
0.0009962841 ABIN3201396 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
0.0009962841 ABIN1959851 ICC IF WB Mouse IgG2b Log in to see 1F3
0.0009962841 ABIN1580427 ICC IF IHC WB Mouse IgG2b Log in to see 1F3
0.0009962841 ABIN4319334 FACS ICC IF SimWes WB Mouse IgG2b Log in to see 1F3
0.0009962841 ABIN4319335 FACS ICC IF SimWes WB Mouse IgG2b Log in to see 1F3
0.0009962841 ABIN4319324 ELISA ICC IF IHC IHC (p) WB Rabbit Center Log in to see Polyclonal
0.0009962841 ABIN4319327 FACS ICC IF IHC IHC (p) WB DyLight 405 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
0.0009962841 ABIN4319383 FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
0.0009962841 ABIN4319384 FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
0.0009962841 ABIN4319385 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
0.0009962841 ABIN4319386 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
0.0009962841 ABIN4221285 ELISA ICC IF IHC WB HRP Rabbit Center Log in to see Polyclonal
0.0009962841 ABIN2973987 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN1951825 ICC IF WB Mouse IgG2b Log in to see

Top referenced anti-HMGB1 antibody for Immunocytochemistry

Similar anti-HMGB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 44 Antibodies 45 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 17 Antibodies 62 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 17 Antibodies 17 Antibodies
Immunofluorescence (IF) 24 Antibodies 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 38 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 89 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope Center
(65), (52), (40), (39), (39), (20), (19), (19), (18), (17), (13), (12), (12), (11), (10), (8), (6), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(400), (187), (118), (34), (30), (22), (14), (13), (6), (6), (3), (1), (1)
Host Rabbit
(232), (179), (4), (4), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(25), (17), (17), (14), (8), (7), (7), (6), (6), (6), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(348), (178), (132), (90), (84), (62), (23), (12), (12), (11), (6), (6), (2), (1), (1), (1), (1)
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling Images
Cross-Reactivity Dog (Canine), Pig (Porcine), Rabbit, Rhesus Monkey, Cow (Bovine)
Cross-Reactivity (Details) Dog (100 %), Pig (100 %), Rabbit (100 %), Rhesus Monkey (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to HMGB1 (high-mobility group box 1)
HMGB1 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.
Isotype IgG

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Cellular Localization: Nucleus
Molecular Weight 25 kDa
Gene ID 3146
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Immunohistochemistry Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* ImmunohistochemistryAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , A375 , NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
Format Liquid
Concentration 1.92 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on mouse esophagus by imm...
Immunofluorescence (IF) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) ICC/IF Image HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent ana...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by western blot analysis. Various whole...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg NIH-...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg PC-1...
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on rat brain stem by immu...
Immunofluorescence (IF) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) ICC/IF Image HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent ana...
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on mouse colon by immunoh...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg 293T...
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) IHC-P Image Immunohistochemical analysis of paraffin-embedded 59T xenograft, using HM...