anti-Mammalian HMGB1 antibody for Cellular Assay

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • hmgb1
  • amphoterin
  • hmg-1
  • hmg1
  • hmg3
  • sbp-1
  • HMGB1
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • p30
  • Ac2-008
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • Hmgb1
  • high-mobility group box 1
  • high mobility group box 1
  • high mobility group protein B1-like
  • high-mobility group box 1a
  • HMG-box transcription factor 1
  • hmgb1
  • HMGB1
  • Hmgb1
  • LOC100392312
  • LOC100754755
  • LOC100359149
  • hmgb1a
  • Hbp1
Reactivity
Mammalian
451
232
161
60
41
37
23
14
13
10
10
10
7
5
2
2
1
Host
Mouse
244
211
3
2
2
2
2
Clonality
Monoclonal
Conjugate
This HMGB1 antibody is un-conjugated
27
20
17
17
9
9
8
8
8
7
7
7
7
6
6
6
5
5
5
4
3
3
1
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition), Cellular Assay (CA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
383
187
137
126
117
85
46
19
13
12
9
8
5
3
2
2
1
1
Supplier
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Catalog No. ABIN1176834
$ 270.57
Plus shipping costs $45.00

Top referenced anti-HMGB1 antibody for Cellular Assay

Similar anti-HMGB1 Antibodies

Application / Reactivity Mammalian
Blocking Antibody (Inhibition) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Western Blotting (WB) 3 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Reactivity Mammalian
(451), (232), (161), (60), (41), (37), (23), (14), (13), (10), (10), (10), (7), (5), (2), (2), (1)
Host Mouse
(244), (211), (3), (2), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HMGB1 antibody is un-conjugated
(27), (20), (17), (17), (9), (9), (8), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), Cellular Assay (CA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(383), (187), (137), (126), (117), (85), (46), (19), (13), (12), (9), (8), (5), (3), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling ProductDetails: References for anti-HMGB1 antibody (ABIN1176834) Images
Immunogen 17-mer peptide KGKPDAAKKGVVKAEKS
Clone DPH1-1
Isotype IgG1

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling ProductDetails: References for anti-HMGB1 antibody (ABIN1176834) Images back to top
Antigen
Alternative Name High-mobility group protein B1 (HMGB1) (HMGB1 Antibody Abstract)
Background DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2. DPH1.1 mAb can be used for Western blot (WB), immunofluorescence (IHF) and immunohistochemistry (IHC). DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays. In vivo, DPH1.1 mAb administered intravenously (220 µg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide KGKPDAAKKGVVKAEKS. Hybridomas were produced from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.
Research Area Neurology
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling ProductDetails: References for anti-HMGB1 antibody (ABIN1176834) Images back to top
Application Notes WB: 1 µg/ml antibody in 5% milk TBS-T overnight at 4°C. Migration assay: 0.5/1/2/5 µg/ml of DPH1.1 mAb is added to 30 ng/ml of rHMGB1, according to Palumbo et al. “Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation”.
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details ProductDetails: References for anti-HMGB1 antibody (ABIN1176834) Images back to top
Format Lyophilized
Handling Advice DPH1.1 mAb is provided freeze-dried.
Storage -20 °C

ProductDetails: References for anti-HMGB1 antibody (ABIN1176834)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Background publications

Scaffidi, Misteli, Bianchi: "Release of chromatin protein HMGB1 by necrotic cells triggers inflammation." in: Nature, Vol. 418, Issue 6894, pp. 191-5, 2002

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling ProductDetails: References for anti-HMGB1 antibody (ABIN1176834) back to top
Supplier Images
 image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN1176834) anti-High-Mobility Group Box 1 (HMGB1) antibody
 image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN1176834) anti-High-Mobility Group Box 1 (HMGB1) antibody (Image 2)
 image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN1176834) Representative immunohistochemistry of HM GB1 (brown) in liver during hepatitis.
 image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN1176834) Western Blot on recombinant HM GB1 and Box A. (B): Western Blot of 100 µg extract. Fr...