anti-Human HRAS antibody for Enzyme Immunoassay

Recommended HRAS Antibody (supplied by: Log in to see )

Antigen
V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) Antibodies
  • H-Ras
  • K-Ras
  • hras1
  • rash1
  • HRAS
  • ras
  • N-Ras
  • c-bas/has
  • hras
  • zgc:110250
  • H-RAS
  • C-BAS/HAS
  • C-H-RAS
  • C-HA-RAS1
  • CTLO
  • H-RASIDX
  • HAMSV
  • HRAS1
  • K-RAS
  • N-RAS
  • RASH1
  • c-H-ras
  • H-ras
  • Ha-ras
  • Harvey-ras
  • Hras-1
  • Kras2
  • c-Ha-ras
  • c-rasHa
  • AI929937
  • K-ras
  • Ki-ras
  • Kras-2
  • p21B
  • Harvey rat sarcoma viral oncogene homolog
  • v-Ha-ras Harvey rat sarcoma viral oncogene homolog
  • v-Ha-ras Harvey rat sarcoma viral oncogene homolog a
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
  • Harvey rat sarcoma virus oncogene
  • Harvey rat sarcoma virus oncogene 1
  • hras
  • HRAS
  • hrasa
  • NRAS
  • KRAS
  • Hras
  • Hras1
  • Kras
Epitope
Middle Region
16
9
6
5
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
122
89
75
11
10
9
2
1
1
1
1
1
1
1
1
Host
Rabbit
110
30
1
1
Clonality
Polyclonal
Conjugate
This HRAS antibody is un-conjugated
9
9
6
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
102
49
26
26
23
19
12
4
3
2
2
1
1
1
Supplier
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Catalog No. ABIN360066
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN952792 EIA IF WB Rabbit Ig C-Term, AA 153-184 Log in to see Polyclonal 5
1 ABIN238418 EIA WB Mouse IgG1 Log in to see H-Ras-03 5

Top referenced anti-HRAS antibody for Enzyme Immunoassay

Similar anti-HRAS Antibodies

Application / Reactivity Human
ELISA 35 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 23 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Neutralization (Neut) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 87 Antibodies

General

Antigen V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) Antibodies
Epitope Middle Region
(16), (9), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(122), (89), (75), (11), (10), (9), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(110), (30), (1), (1)
Clonality
Polyclonal
Conjugate This HRAS antibody is un-conjugated
(9), (9), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(102), (49), (26), (26), (23), (19), (12), (4), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HRAS Antibody

Target Details HRAS Application Details Handling ProductDetails: References for anti-HRAS antibody (ABIN360066) Images
Specificity This antibody reacts to HRAS.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human HRAS.
Isotype Ig

Target Details HRAS

Product Details anti-HRAS Antibody Application Details Handling ProductDetails: References for anti-HRAS antibody (ABIN360066) Images back to top
Antigen
Alternative Name HRAS (HRAS Antibody Abstract)
Background HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. These proteins function in signal transduction pathways. They can bind GTP and GDP, and they have intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in the HRAS gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma.Synonyms: GTPase HRas, H-Ras, H-Ras-1, HRAS1, Ha-Ras, c-H-ras, p21ras, v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Gene ID 3265, 9606
UniProt P01112
Pathways p53 Signaling, MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-HRAS Antibody Target Details HRAS Handling ProductDetails: References for anti-HRAS antibody (ABIN360066) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HRAS Antibody Target Details HRAS Application Details ProductDetails: References for anti-HRAS antibody (ABIN360066) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-HRAS antibody (ABIN360066)

Product Details anti-HRAS Antibody Target Details HRAS Application Details Handling Images back to top
Background publications

Winter-Vann, Kamen, Bergo, Young, Melnyk, James, Casey: "Targeting Ras signaling through inhibition of carboxyl methylation: an unexpected property of methotrexate." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 11, pp. 6529-34, 2003

Coats, Booden, Buss: "Transient palmitoylation supports H-Ras membrane binding but only partial biological activity." in: Biochemistry, Vol. 38, Issue 39, pp. 12926-34, 1999

Sakai, Rikimaru, Ueda, Matsumoto, Ishii, Enomoto, Yamamoto, Tsuchida: "The p53 tumor-suppressor gene and ras oncogene mutations in oral squamous-cell carcinoma." in: International journal of cancer. Journal international du cancer, Vol. 52, Issue 6, pp. 867-72, 1993

Images

Product Details anti-HRAS Antibody Target Details HRAS Application Details Handling ProductDetails: References for anti-HRAS antibody (ABIN360066) back to top
Supplier Images
 image for anti-HRAS antibody (V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog) (Middle Region) (ABIN360066) anti-V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) (Middle Region) antibody
 image for anti-HRAS antibody (V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog) (Middle Region) (ABIN360066) anti-V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) (Middle Region) antibody (Image 2)