anti-Human p53 antibody for Immunohistochemistry (Frozen Sections)

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
full length
170
118
84
83
69
65
55
55
54
53
49
47
46
45
40
32
31
31
25
23
23
22
20
18
17
16
15
15
15
15
15
14
11
10
9
9
9
9
8
8
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2329
513
418
186
127
94
85
82
34
23
20
18
17
16
15
14
6
4
4
4
4
3
3
3
2
2
1
1
Host
Mouse
1322
1112
7
3
3
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
78
76
63
53
45
44
30
30
25
25
25
24
24
24
24
23
23
23
21
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
1856
746
719
715
614
596
423
340
299
179
72
41
32
31
18
17
15
15
13
9
8
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN967416
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1108553 IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see IMD-53 2
1 ABIN120054 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 3
1 ABIN4342783 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12 1
1 ABIN152249 CyTOF ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 20
1 ABIN120058 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 181-190 Log in to see DO-11 1
1 ABIN111971 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG2a AA 16-25 Log in to see BP53-12 8
1 ABIN320175 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 4
1 ABIN967420 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Log in to see PAb 240 7
1 ABIN967419 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Log in to see PAb 240 7
1 ABIN152250 ChIP FACS IHC (fro) IHC (p) IP IHC RIA ELISA WB Mouse IgG1 Log in to see Pab1801 7
1 ABIN967458 IHC (f) IHC (fro) ICS IP WB Mouse IgG2b Log in to see DO 7
1 ABIN967457 IHC (f) IHC (fro) IP WB Mouse IgG2a Log in to see DO 8
1 ABIN4219254 ELISA IHC (fp) IHC (fro) IP WB Mouse IgG2a AA 20-31 Log in to see Bp53-11 9
1 ABIN967421 IHC (fro) IF IHC (p) IP WB Mouse IgG1 Log in to see PAb 1801 9
1 ABIN967422 IHC (f) IHC (fro) IF IP WB Mouse IgG1 Log in to see PAb 1801 9
1 ABIN3043664 ICC IHC (fro) IHC (p) WB Mouse IgG2a Log in to see BP53-12 28
1 ABIN264449 EIA IHC (fro) IP WB Mouse IgG2b AA 371-380 Log in to see HR231
1 ABIN356316 FACS IHC (fro) IF IHC (p) Mouse IgG2a Log in to see BP53-12 1
1 ABIN452450 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG2a AA 20-31 Log in to see Bp53-11 8
1 ABIN2476020 ELISA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 4

Top referenced anti-p53 antibody for Immunohistochemistry (Frozen Sections)

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Application / Reactivity Rat (Rattus)
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Cytometry by Time of Flight (CyTOF) 4 Antibodies
ELISA 128 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 72 Antibodies
Gel Shift (GS) 3 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 64 Antibodies
Immunofluorescence (IF) 116 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 73 Antibodies
Immunohistochemistry (IHC) 104 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 59 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 78 Antibodies
Immunoprecipitation (IP) 68 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 298 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope full length
(170), (118), (84), (83), (69), (65), (55), (55), (54), (53), (49), (47), (46), (45), (40), (32), (31), (31), (25), (23), (23), (22), (20), (18), (17), (16), (15), (15), (15), (15), (15), (14), (11), (10), (9), (9), (9), (9), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(2329), (513), (418), (186), (127), (94), (85), (82), (34), (23), (20), (18), (17), (16), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(1322), (1112), (7), (3), (3), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(78), (76), (63), (53), (45), (44), (30), (30), (25), (25), (25), (24), (24), (24), (24), (23), (23), (23), (21), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
(1856), (746), (719), (715), (614), (596), (423), (340), (299), (179), (72), (41), (32), (31), (18), (17), (15), (15), (13), (9), (8), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN967416) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant full-length human p53
Clone G59-12
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN967416) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background P53 is a 53 kD nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and the wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the S-phase of the cell cycle. p53 migrates at a reduced molecular weight of 53 kDa. Clone G59-12 recognizes mutant and wild type human, rat and mouse p53 tumor suppressor protein. Recombinant full-length human p53 was used as immunogen.
Molecular Weight 53 kDa
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling ProductDetails: References for anti-p53 antibody (ABIN967416) Images back to top
Application Notes Clone G59-12 conjugated to R-Phycoerythrin (PE) is suggested for flow cytometric analysis of p53. Positive control cell lines include SKBR-3 human breast carcinoma cells (ATCC HTB-30) and A431 human vulval carcinoma cells (ATCC CRL-1555). Jurkat T cells (ATCC TIB-152) or MCF-7 human breast carcinoma cells (ATCC HTB-22) are suggested as negative controls. Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach, or breast tissue.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details ProductDetails: References for anti-p53 antibody (ABIN967416) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

ProductDetails: References for anti-p53 antibody (ABIN967416)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Van Meir, Roemer, Diserens, Kikuchi, Rempel, Haas, Huang, Friedmann, de Tribolet, Cavenee: "Single cell monitoring of growth arrest and morphological changes induced by transfer of wild-type p53 alleles to glioblastoma cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 4, pp. 1008-12, 1995

Jacquemier, Molès, Penault-Llorca, Adélaide, Torrente, Viens, Birnbaum, Theillet: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, 1994

Mørkve, Halvorsen, Stangeland, Gulsvik, Laerum: "Quantitation of biological tumor markers (p53, c-myc, Ki-67 and DNA ploidy) by multiparameter flow cytometry in non-small-cell lung cancer." in: International journal of cancer. Journal international du cancer, Vol. 52, Issue 6, pp. 851-5, 1993

van den Berg, Baas, Polak, Offerhaus: "Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid." in: The American journal of pathology, Vol. 142, Issue 2, pp. 381-5, 1993

Yeargin, Cheng, Yu, Gjerset, Bogart, Haas: "P53 mutation in acute T cell lymphoblastic leukemia is of somatic origin and is stable during establishment of T cell acute lymphoblastic leukemia cell lines." in: The Journal of clinical investigation, Vol. 91, Issue 5, pp. 2111-7, 1993

Cheng, Yee, Yeargin, Friedmann, Haas: "Suppression of acute lymphoblastic leukemia by the human wild-type p53 gene." in: Cancer research, Vol. 52, Issue 1, pp. 222-6, 1992

Yeargin, Cheng, Haas: "Role of the p53 tumor suppressor gene in the pathogenesis and in the suppression of acute lymphoblastic T-cell leukemia." in: Leukemia, Vol. 6 Suppl 3, pp. 85S-91S, 1992

Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991

Background publications

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992

Gjerset, Arya, Volkman, Haas: "Association of induction of a fully tumorigenic phenotype in murine radiation-induced T-lymphoma cells with loss of differentiation antigens, gain of CD44, and alterations in p53 protein levels." in: Molecular carcinogenesis, Vol. 5, Issue 3, pp. 190-8, 1992

Stein, Stoica, Tilley, Burghardt: "Rat ovarian granulosa cell culture: a model system for the study of cell-cell communication during multistep transformation." in: Cancer research, Vol. 51, Issue 2, pp. 696-706, 1991

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN967416) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (full length) (ABIN967416) Western blot analysis of p53. A SV-40 transformed rat granulosa cell lysate was probe...
 image for anti-p53 antibody (Tumor Protein P53) (full length) (ABIN967416) anti-Tumor Protein P53 (TP53) (full length) antibody (Image 2)
'Independent Validation' Badge
Lot Number 2335876
Method validated Western Blotting
Positive Control U251-MG cells
Negative Control PC3 cells
Notes Strong bands of the expected size were observed in the positive control sample, and not in the negative control sample.
Primary Antibody
  • Antibody: Tumor Protein P53 (TP53) antibody
  • Catalog number: ABIN967416
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 2335876
Secondary Antibody
Controls
  • U251-MG (positive) and PC3 (negative) cell line extracts were prepared using RIPA buffer (R0278, Sigma Aldrich).
  • Loading control: blots were stripped and re-probed for beta-actin to ensure equal loading of lysates.
Protocol
  • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25%
  • Beta-mercaptoethanol at 95°C for 5 min prior to loading.
  • 2. 32 µg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
  • 3. The Spectra Multicolor Broad Range molecular mass marker (26634 Thermo Scientific) was used as a
  • standard.
  • 4. Proteins were then transferred onto PVDF membrane by tank transfer and protein transfer was
  • confirmed with Ponceau S staining.
  • 5. The immunoblot membrane was blocked in PBS containing 3% (W/V) non-fat dry milk at room
  • temperature for 1 h.
  • 6. The membrane was rinsed with PBS containing 0.05% Tween-20 once.
  • 7. The membrane was immersed with the protein side up in the antibody solution in PBS containing 1%
  • (W/V) non-fat dry milk and incubated for 2 h at room temperature (~26°C).
  • 8. The membrane was rinsed in PBS containing 0.05% Tween-20 thrice for 10 min each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in PBS containing
  • 1% (W/V) non-fat dry milk and incubated for 1 h at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice PBS containing 0.05% Tween-20 thrice for 10 min each.
  • 11. The membrane was washed in PBS twice for 30 sec each.
  • 12. Signals were detected with Pierce ECL Western Blotting Substrate (32109, Thermo Scientific). The
  • blot was scanned for 300 sec.
  • 13. The membrane was rinsed three times with PBS containing 0.05% Tween-20.4. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10
  • min each.
  • 15. The membrane was washed in PBS containing 0.05% Tween-20 times for 10 min each.
  • 16. Repeated Steps 5-12 with the loading control antibody (beta-actin) and its matching secondary
  • antibody.
Experimental Notes None
Validation Images
Western Blotting validation image for anti-Tumor Protein P53 (TP53) (full length) antibody (ABIN967416) Figure 1: Western blot analysis of U251-MG and PC3 cell line extracts using Tumor Pro...