anti-Human p53 antibody for Radioimmunoassay

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 46-53
173
128
94
84
74
70
70
62
59
55
50
49
48
47
42
38
32
31
29
28
24
22
20
20
17
16
15
15
15
15
15
14
12
11
10
10
10
9
9
8
7
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2428
519
426
188
127
94
87
84
35
23
20
18
17
16
15
14
6
4
4
4
4
3
3
3
2
2
1
1
Host
Mouse
1372
1169
7
3
1
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
81
78
65
55
49
48
34
34
26
26
25
25
25
25
25
24
24
24
23
22
22
21
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
1931
771
750
742
650
637
434
347
316
179
71
41
32
31
24
17
17
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN863095
$ 321.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN152250 ChIP FACS IHC (fro) IHC (p) IP IHC RIA ELISA WB Mouse IgG1 Log in to see Pab1801 7
1 ABIN2482441 FACS IP RIA IHC ELISA WB Atto 390 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482456 FACS IP RIA IHC ELISA WB PE Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482445 FACS IP RIA IHC ELISA WB Atto 633 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482454 FACS IP RIA IHC ELISA WB PE-Atto 594 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482457 FACS IP RIA IHC ELISA WB Streptavidin Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482442 FACS IP RIA IHC ELISA WB Atto 488 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482449 FACS IP RIA IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482450 FACS IP RIA IHC ELISA WB APC Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482453 FACS IP RIA IHC ELISA WB HRP Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482452 FACS IP RIA IHC ELISA WB FITC Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482455 FACS IP RIA IHC ELISA WB PerCP Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482443 FACS IP RIA IHC ELISA WB Atto 565 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482444 FACS IP RIA IHC ELISA WB Atto 594 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482447 FACS IP RIA IHC ELISA WB Atto 680 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482446 FACS IP RIA IHC ELISA WB Atto 655 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482448 FACS IP RIA IHC ELISA WB Atto 700 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482451 FACS IP RIA IHC ELISA WB Biotin Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN5550996 EIA FACS IHC (fro) IHC (p) IP RIA WB Mouse IgG1 AA 46-53 Log in to see Pab 1801
1 ABIN1943386 IHC (fro) FACS IP IHC (p) RIA ELISA WB Atto 390 Mouse IgG1 AA 46-53 Log in to see Pab 1801

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 46-53
(173), (128), (94), (84), (74), (70), (70), (62), (59), (55), (50), (49), (48), (47), (42), (38), (32), (31), (29), (28), (24), (22), (20), (20), (17), (16), (15), (15), (15), (15), (15), (14), (12), (11), (10), (10), (10), (9), (9), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2428), (519), (426), (188), (127), (94), (87), (84), (35), (23), (20), (18), (17), (16), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(1372), (1169), (7), (3), (1), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(81), (78), (65), (55), (49), (48), (34), (34), (26), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (22), (22), (21), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(1931), (771), (750), (742), (650), (637), (434), (347), (316), (179), (71), (41), (32), (31), (24), (17), (17), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 antibody (ABIN863095) Images
Specificity Detects ~53 kDa.
Purification Protein G Purified
Immunogen Fusion protein (Human), amino acids 46-53
Clone Pab 1801
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 antibody (ABIN863095) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background The p53 protein (tumor protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumor suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50 % of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5).
Gene ID 7157
NCBI Accession NP_000537
UniProt P04637
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 antibody (ABIN863095) Images back to top
Application Notes
  • WB (1:1000)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-183 was sufficient for detection of p53 in 10 μg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 antibody (ABIN863095) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-p53 antibody (ABIN863095)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Hansen, Fischer, Chan, Chang, Baldwin, Aragon, Carter, Lilly, Nishimura, Weisbart, Reeves: "Antibody-mediated p53 protein therapy prevents liver metastasis in vivo." in: Cancer research, Vol. 67, Issue 4, pp. 1769-74, 2007

Georgescu, Săftoiu, Georgescu, Ciurea, Ciurea: "Correlations of proliferation markers, p53 expression and histological findings in colorectal carcinoma." in: Journal of gastrointestinal and liver diseases : JGLD, Vol. 16, Issue 2, pp. 133-9, 2007

Akere, Otegbayo: "Evaluation of the pattern and prognostic implications of anti-p53 in hepatocellular carcinoma." in: Singapore medical journal, Vol. 48, Issue 1, pp. 41-4, 2007

Di Cesare, Previti, Lombardo, Di Benedetto, Mazzù, Romano, De Luca, Lasco, Cucinotta: "Serum anti-p53 autoantibodies in patients with type 1 diabetes." in: Annals of clinical and laboratory science, Vol. 31, Issue 3, pp. 253-8, 2001

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 antibody (ABIN863095) back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 46-53) antibody (ABIN863095) p53 Hela 20ug 1 in 1000 Western Blotting.