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|Application / Reactivity||Human|
|Affinity Purification (AP)||2 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Chromatin Immunoprecipitation (ChIP)||10 Antibodies|
|Dot Blot (DB)||31 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||2 Antibodies|
|Enzyme Immunoassay (EIA)||17 Antibodies|
|Flow Cytometry (FACS)||220 Antibodies|
|Gel Shift (GS)||3 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||35 Antibodies|
|Immunocytochemistry (ICC)||78 Antibodies|
|Immunodiffusion (ID)||16 Antibodies|
|Immunofluorescence (IF)||309 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||160 Antibodies|
|Immunohistochemistry (IHC)||510 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||11 Antibodies|
|Antigen||Tumor Protein P53 (TP53) Antibodies|
|Epitope||AA 46-53 Alternatives|
|Conjugate||This p53 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
|4 references available|
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Product Details anti-p53 AntibodyTarget Details p53 Application Details Handling References for anti-p53 Antibody (ABIN863095) Images
|Specificity||Detects ~53 kDa.|
|Sensitivity||1 µg/mL of SMC-183 was sufficient for detection of p53 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|
|Purification||Protein G Purified|
|Immunogen||Fusion protein (Human), amino acids 46-53|
Target Details p53Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN863095) Images back to top
|Alternative Name||p53 (TP53 Antibody Abstract)|
The p53 protein (tumor protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumor suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50 % of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5).
Cellular Localization: Cytoplasm | Nucleus | PML body | Endoplasmic Reticulum
|Research Area||Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis|
|Pathways||p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling|
Application DetailsProduct Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN863095) Images back to top
|Application Notes||Recommended Dilution: WB (1:1000), optimal dilutions for assays should be determined by the user.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN863095) Images back to top
|Buffer||PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
References for anti-p53 Antibody (ABIN863095)Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Georgescu, Săftoiu, Georgescu et al.: "Correlations of proliferation markers, p53 expression and histological findings in colorectal carcinoma." in: Journal of gastrointestinal and liver diseases : JGLD, Vol. 16, Issue 2, pp. 133-9, 2007 (PubMed).
Hansen, Fischer, Chan et al.: "Antibody-mediated p53 protein therapy prevents liver metastasis in vivo." in: Cancer research, Vol. 67, Issue 4, pp. 1769-74, 2007 (PubMed).
Akere, Otegbayo: "Evaluation of the pattern and prognostic implications of anti-p53 in hepatocellular carcinoma." in: Singapore medical journal, Vol. 48, Issue 1, pp. 41-4, 2007 (PubMed).
Di Cesare, Previti, Lombardo et al.: "Serum anti-p53 autoantibodies in patients with type 1 diabetes." in: Annals of clinical and laboratory science, Vol. 31, Issue 3, pp. 253-8, 2001 (PubMed).
ImagesProduct Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN863095) back to top