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anti-Mouse (Murine) p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
pSer20
123
87
62
53
51
50
49
48
46
46
43
40
39
30
27
25
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
8
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
1760
318
269
99
34
19
13
11
11
10
10
9
5
4
4
4
3
3
3
2
2
2
1
Host
Rabbit
1234
613
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
59
49
34
31
23
23
15
15
14
14
14
14
14
14
13
4
4
4
4
4
4
4
4
4
4
3
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1378
582
529
319
315
302
235
156
99
87
53
39
35
17
16
12
12
11
11
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN498795
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.33521742 ABIN499003 IHC (p) WB Rabbit Log in to see Polyclonal
0.33521742 ABIN499004 IHC (p) WB Rabbit Log in to see Polyclonal
0.33521742 ABIN498159 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.33521742 ABIN498886 IHC (p) WB Rabbit Log in to see Polyclonal
0.00090312806 ABIN358195 EIA IHC (p) WB Rabbit Ig pThr18 Log in to see Polyclonal 5
0.00090312806 ABIN967420 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Log in to see PAb 240 7
0.00090312806 ABIN967419 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Log in to see PAb 240 7
0.00090312806 ABIN723935 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 240-290, Wild Type Log in to see Polyclonal 1
0.00090312806 ABIN677408 IF (p) IHC (p) WB Rabbit IgG AA 345-390, Wild Type Log in to see Polyclonal 1
0.00090312806 ABIN319211 IHC (p) WB Rabbit pSer6 Log in to see Polyclonal 4
0.00090312806 ABIN272049 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.00090312806 ABIN272050 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.00090312806 ABIN272156 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.00090312806 ABIN127171 IHC (p) IP WB Rabbit pSer15 Log in to see Polyclonal
0.00090312806 ABIN389626 DB IHC (p) WB Rabbit Ig pThr18 Log in to see Polyclonal
0.00090312806 ABIN126922 IF IHC (p) IP WB Mouse IgG1 Ser20 Log in to see C6A5
0.00090312806 ABIN702895 IF (p) IHC (p) WB Rabbit IgG AA 5-55, pSer20 Log in to see Polyclonal
0.00090312806 ABIN383969 FACS IHC (p) IP WB Mouse IgG1 AA 14-289 Log in to see PAb240
0.00090312806 ABIN317853 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.00090312806 ABIN1740217 ELISA ICC IF IHC (p) Rabbit IgG Lys379, acLys379 Log in to see Polyclonal

Top referenced anti-p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-p53 Antibodies

Application / Reactivity Mouse (Murine) Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 11 Antibodies
ELISA 73 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 30 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 58 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunohistochemistry (IHC) 74 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 45 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 222 Antibodies
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope pSer20
(123), (87), (62), (53), (51), (50), (49), (48), (46), (46), (43), (40), (39), (30), (27), (25), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(1760), (318), (269), (99), (34), (19), (13), (11), (11), (10), (10), (9), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1)
Host Rabbit
(1234), (613), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(59), (49), (34), (31), (23), (23), (15), (15), (14), (14), (14), (14), (14), (14), (13), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1378), (582), (529), (319), (315), (302), (235), (156), (99), (87), (53), (39), (35), (17), (16), (12), (12), (11), (11), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Specificity This antibody detects endogenous levels of p-P53 protein only when phosphorylated at Ser20.
Cross-Reactivity (Details) Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background P53 is a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers. Phosphorylation of p53 at residue Thr 155 is mediated by the COP9 signalosome (CSN) and targets p53 to ubiquitin-26S Proteasome-dependent degradation.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Molecular Weight approx. 53,43 kDa
Gene ID 7157
NCBI Accession NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p53 antibody (Tumor Protein P53) (pSer20) (ABIN498795) Immunohistochemistry (IHC) analyzes of p-P53 antibody in paraffin-embedded hum...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (pSer20) (ABIN498795) Western blot (WB) analysis of p-P53 antibody in extracts from COS7 cells.