anti-Pig (Porcine) p53 antibody for Western Blotting

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
N-Term
170
117
84
83
69
65
55
55
54
53
49
47
46
45
40
32
31
31
25
23
23
22
20
18
17
16
15
15
15
15
15
14
11
10
10
9
9
9
8
8
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
2329
514
418
186
126
94
85
81
34
22
19
18
16
16
15
13
6
4
4
4
4
3
3
2
2
2
1
1
Host
Rabbit
1321
1113
7
3
3
1
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
78
76
63
53
45
44
30
30
25
25
25
24
24
24
24
23
23
23
21
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Western Blotting (WB)
1856
746
719
714
614
596
424
340
300
179
72
41
32
31
18
16
15
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN2779317
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3484411 ABIN2777951 WB Rabbit N-Term Log in to see Polyclonal 1
3.3484411 ABIN2779979 WB Rabbit C-Term Log in to see Polyclonal 1
3.3484411 ABIN1583540 ELISA WB Rabbit C-Term Log in to see Polyclonal
3.3484411 ABIN2957711 WB Rabbit C-Term Log in to see Polyclonal
3.3484411 ABIN2957708 WB Rabbit C-Term, acLys370 Log in to see Polyclonal
3.3484411 ABIN2957704 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
3.3484411 ABIN2707675 WB Rabbit N-Term Log in to see Polyclonal
3.3484411 ABIN3222958 WB Rabbit 3meLys370, C-Term Log in to see Polyclonal
1 ABIN202409 WB Rabbit IgG AA 281-330 Log in to see Polyclonal
1 ABIN467883 WB Rabbit N-Term Log in to see Polyclonal
1 ABIN574482 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN187565 IP WB Mouse IgG1 Mutant, AA 213-217 Log in to see Pab 240 2
1 ABIN2755791 IC IF IHC WB Rabbit N-Term, pSer20 Log in to see Polyclonal
1 ABIN2755790 WB Rabbit N-Term, pSer18 Log in to see Polyclonal
1 ABIN5514886 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2575425 WB Rabbit N-Term Log in to see Polyclonal
1 ABIN3066409 WB Rabbit N-Term, pThr18 Log in to see Polyclonal
1 ABIN3066411 IF/ICC IHC WB Rabbit N-Term, pSer20 Log in to see Polyclonal

Similar anti-p53 Antibodies

Application / Reactivity Human Rat (Rattus) Cow (Bovine) Pig (Porcine) Rabbit Dog (Canine) Horse (Equine) Sheep (Ovine)
Immunofluorescence (fixed cells) (IF/ICC) 28 Antibodies 13 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 18 Antibodies 4 Antibodies 4 Antibodies 6 Antibodies
In Vivo Imaging (IVI) 1 Antibodies
Simple Western (SimWes) 9 Antibodies 3 Antibodies 3 Antibodies
Western Blotting (WB) 1773 Antibodies 100 Antibodies 9 Antibodies 8 Antibodies 298 Antibodies 54 Antibodies 19 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope N-Term
(170), (117), (84), (83), (69), (65), (55), (55), (54), (53), (49), (47), (46), (45), (40), (32), (31), (31), (25), (23), (23), (22), (20), (18), (17), (16), (15), (15), (15), (15), (15), (14), (11), (10), (10), (9), (9), (9), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(2329), (514), (418), (186), (126), (94), (85), (81), (34), (22), (19), (18), (16), (16), (15), (13), (6), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(1321), (1113), (7), (3), (3), (1), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(78), (76), (63), (53), (45), (44), (30), (30), (25), (25), (25), (24), (24), (24), (24), (23), (23), (23), (21), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Western Blotting (WB)
(1856), (746), (719), (714), (614), (596), (424), (340), (300), (179), (72), (41), (32), (31), (18), (16), (15), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN2779317) Images
Blocking Peptide Blocking peptide for this product available: ABIN985382
Sequence EEPQSDPSVE PPLSQETFSD LWKLLPENNV LSPLPSQAMD DLMLSPDDIE
Predicted Reactivity Cow: 100%, Dog: 80%, Guinea Pig: 90%, Horse: 90%, Human: 100%, Pig: 100%, Rabbit: 100%, Rat: 90%, Sheep: 100%
Characteristics This is a rabbit polyclonal antibody against TP53. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TP53

Target Details p53

Product Details anti-p53 Antibody Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN2779317) Images back to top
Antigen
Alternative Name TP53 (TP53 Antibody Abstract)
Background TP53 acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.
Alias Symbols: LFS1, TRP53, p53
Protein Size: 393
Molecular Weight 44 kDa
Gene ID 7157
NCBI Accession NM_000546, NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling ProductDetails: References for anti-p53 antibody (ABIN2779317) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 393 AA

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details ProductDetails: References for anti-p53 antibody (ABIN2779317) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-p53 antibody (ABIN2779317)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Boehme, Kulikov, Blattner: "p53 stabilization in response to DNA damage requires Akt/PKB and DNA-PK." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 22, pp. 7785-90, 2008

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN2779317) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) anti-Tumor Protein P53 (TP53) (N-Term) antibody
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) Host: Rabbit
Target Name: TP53
Sample Tissue: Human Fetal Liver
...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) Host: Rabbit
Target Name: TP53
Sample Tissue: Human Adult Placenta