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anti-Pig (Porcine) p53 antibody for Western Blotting

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
N-Term
123
86
62
54
51
50
49
48
46
46
43
40
39
30
27
25
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
8
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Pig (Porcine), Rabbit
1760
319
269
99
33
18
12
11
11
10
10
9
5
4
4
4
3
3
3
2
2
2
1
Host
Rabbit
1234
613
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
59
49
34
31
23
23
15
15
14
14
14
14
14
14
13
4
4
4
4
4
4
4
4
4
4
3
1
1
Application
Western Blotting (WB)
1378
582
529
319
315
303
235
156
99
87
53
39
35
17
16
12
12
11
11
9
4
3
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN2777951
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.117733724 ABIN2779317 ChIP IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.117733724 ABIN2779979 WB Rabbit C-Term Log in to see Polyclonal 1
0.117733724 ABIN2792141 WB Rabbit C-Term Log in to see Polyclonal 1
0.117733724 ABIN1583540 ELISA WB Rabbit C-Term Log in to see Polyclonal
0.117733724 ABIN2707675 WB Rabbit N-Term Log in to see Polyclonal
0.0011849351 ABIN187565 IP WB Mouse IgG1 Mutant, AA 213-217 Log in to see Pab 240 2
0.0011849351 ABIN2755791 IC IF IHC WB Rabbit N-Term, pSer20 Log in to see Polyclonal
0.0011849351 ABIN2755790 WB Rabbit N-Term, pSer18 Log in to see Polyclonal
0.0011849351 ABIN2957704 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
0.0011849351 ABIN2957708 WB Rabbit C-Term, acLys370 Log in to see Polyclonal
0.0011849351 ABIN2957711 WB Rabbit C-Term Log in to see Polyclonal
0.0011849351 ABIN3222958 WB Rabbit 3meLys370, C-Term Log in to see Polyclonal
0.0011849351 ABIN3066409 WB Rabbit N-Term, pThr18 Log in to see Polyclonal
0.0011849351 ABIN3066411 ICC IF IHC WB Rabbit N-Term, pSer20 Log in to see Polyclonal

Top referenced anti-p53 antibody for Western Blotting

Similar anti-p53 Antibodies

Application / Reactivity Human Cow (Bovine) Pig (Porcine) Rabbit
Western Blotting (WB) 1313 Antibodies 21 Antibodies 15 Antibodies 11 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Radioimmunoassay (RIA) 54 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies
Intracellular Staining (ICS) 3 Antibodies 1 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 290 Antibodies 9 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 301 Antibodies 8 Antibodies
Immunohistochemistry (IHC) 515 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope N-Term
(123), (86), (62), (54), (51), (50), (49), (48), (46), (46), (43), (40), (39), (30), (27), (25), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Pig (Porcine), Rabbit
(1760), (319), (269), (99), (33), (18), (12), (11), (11), (10), (10), (9), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1)
Host Rabbit
(1234), (613), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(59), (49), (34), (31), (23), (23), (15), (15), (14), (14), (14), (14), (14), (14), (13), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1)
Application Western Blotting (WB)
(1378), (582), (529), (319), (315), (303), (235), (156), (99), (87), (53), (39), (35), (17), (16), (12), (12), (11), (11), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2777951) Images
Blocking Peptide Blocking peptide for this product available: ABIN985387
Sequence EEPQSDPSVE PPLSQETFSD LWKLLPENNV LSPLPSQAMD DLMLSPDDIE
Predicted Reactivity Bovine : 100 %, Human : 100 %, Horse : 90 %
Characteristics This is a rabbit polyclonal antibody against TP53. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TP53

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN2777951) Images back to top
Antigen
Alternative Name TP53 (TP53 Antibody Abstract)
Background TP53 acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.
Molecular Weight 44 kDa
Gene ID 7157
NCBI Accession NM_000546, NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN2777951) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 393 AA

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN2777951) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-p53 Antibody (ABIN2777951)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Boehme, Kulikov, Blattner: "p53 stabilization in response to DNA damage requires Akt/PKB and DNA-PK." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 22, pp. 7785-90, 2008

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2777951) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2777951) WB Suggested Anti-TP53 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2777951) Sample Type: 1. and 2. Human Cystic Fibrosis Bronchial Epithelial Cells (35ug)...