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anti-Primate p53 antibody for Immunofluorescence

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 16-25
152
99
84
68
64
64
55
54
54
51
49
45
43
41
31
30
29
28
24
23
23
21
20
19
17
17
15
15
15
15
15
14
11
10
9
9
9
8
8
7
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Primate
2131
480
395
169
127
93
68
64
34
23
18
17
16
16
15
11
6
4
4
4
4
3
3
2
2
1
Host
Mouse
1253
968
7
3
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
71
68
56
47
36
35
24
24
21
21
19
18
18
18
18
17
17
17
17
16
16
15
14
14
14
14
14
12
7
4
4
4
4
4
4
4
4
3
3
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1719
716
636
579
526
466
399
265
250
179
55
41
32
31
18
16
15
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN111971
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN111970 IF FITC Mouse IgG2a AA 16-25 Log in to see BP53-12 8
1 ABIN231628 FACS IF FITC Mouse IgG2a AA 16-25 Log in to see BP53-12
1 ABIN4342794 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342793 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342845 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342840 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342841 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342842 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342855 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342857 ICC IF IHC (fro) IHC (p) WB Biotin Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342849 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342853 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342858 FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342859 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342860 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342862 FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342865 FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342869 ELISA ICC IF IHC (fro) IHC (p) WB Biotin Mouse IgG2b kappa AA 37-45 Log in to see DO-7
1 ABIN4342872 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgG2b kappa AA 37-45 Log in to see DO-7
1 ABIN4342870 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG2b kappa AA 37-45 Log in to see DO-7

Top referenced anti-p53 antibody for Immunofluorescence

Similar anti-p53 Antibodies

Application / Reactivity Primate Human
Cytometry by Time of Flight (CyTOF) 8 Antibodies 18 Antibodies
ELISA 53 Antibodies 666 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 77 Antibodies
Immunocytochemistry (ICC) 74 Antibodies
Immunofluorescence (IF) 74 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 75 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 62 Antibodies
Immunoprecipitation (IP) 25 Antibodies
Simple Western (SimWes) 3 Antibodies
Western Blotting (WB) 81 Antibodies
Affinity Purification (AP) 2 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 14 Antibodies
Dot Blot (DB) 27 Antibodies
ELISA (Capture) 2 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 16-25
(152), (99), (84), (68), (64), (64), (55), (54), (54), (51), (49), (45), (43), (41), (31), (30), (29), (28), (24), (23), (23), (21), (20), (19), (17), (17), (15), (15), (15), (15), (15), (14), (11), (10), (9), (9), (9), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Primate
(2131), (480), (395), (169), (127), (93), (68), (64), (34), (23), (18), (17), (16), (16), (15), (11), (6), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Host Mouse
(1253), (968), (7), (3), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(71), (68), (56), (47), (36), (35), (24), (24), (21), (21), (19), (18), (18), (18), (18), (17), (17), (17), (17), (16), (16), (15), (14), (14), (14), (14), (14), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1719), (716), (636), (579), (526), (466), (399), (265), (250), (179), (55), (41), (32), (31), (18), (16), (15), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN111971) Images
Specificity This Monoclonal antibody BP53-12 recognizes defined epitope (aa 16-25) on Human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells. It is frequently mutated or inactivated in many types of cancer.
Purification Precipitation Methods
Immunogen Bacterially expressed full-length wild-type p53.
Clone BP53-12
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN111971) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Gene ID 7157
NCBI Accession NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN111971) Images back to top
Application Notes ELISA. Western Blotting (Non-reducing conditions): Recommended Dilution: 1-2 μg/mL, overnight in 4 °CPositive Control: RAMOS Human lymphoma cell lineSample Preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer(1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. SDS-PAGE: 12 % separating gel. Immunoprecipitation. Immunocytochemistry. Immunohistochemistry on Frozen and Paraffin-Embedded Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN111971) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-p53 Antibody (ABIN111971)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Tanigawa, Fujii, Hou: "Stabilization of p53 is involved in quercetin-induced cell cycle arrest and apoptosis in HepG2 cells." in: Bioscience, biotechnology, and biochemistry, Vol. 72, Issue 3, pp. 797-804, 2008

Taylor, DePrimo, Agarwal, Agarwal, Schönthal, Katula, Stark: "Mechanisms of G2 arrest in response to overexpression of p53." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3607-22, 1999

Agarwal, Agarwal, Taylor, Chernova, Sharma, Stark: "A p53-dependent S-phase checkpoint helps to protect cells from DNA damage in response to starvation for pyrimidine nucleotides." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 25, pp. 14775-80, 1999

Taylor, Agarwal, Agarwal, Stacey, Stark: "p53 inhibits entry into mitosis when DNA synthesis is blocked." in: Oncogene, Vol. 18, Issue 2, pp. 283-95, 1999

Agarwal, Agarwal, Taylor, Stark: "p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 18, pp. 8493-7, 1995

Bártek, Bártková, Vojt?sek, Stasková, Lukás, Rejthar, Kovarík, Midgley, Gannon, Lane: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." in: Oncogene, Vol. 6, Issue 9, pp. 1699-703, 1991

Product cited in:

Dolezalová, Vojt?sek, Kovarík: "Epitope analysis of the human p53 tumour suppressor protein." in: Folia biologica, Vol. 43, Issue 1, pp. 49-51, 1997

Bártková, Bártek, Lukás, Vojt?sek, Stasková, Rejthar, Kovarík, Midgley, Lane: "p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 2, pp. 196-202, 1991