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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||1572 Antibodies||282 Antibodies||224 Antibodies|
|Transfected Cell Culture (TCC)||1 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Radioimmunoassay (RIA)||54 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||4 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||1 Antibodies||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||4 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||402 Antibodies||82 Antibodies||72 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||486 Antibodies||81 Antibodies|
|Antigen||Tumor Protein P53 (TP53) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This p53 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-p53 AntibodyTarget Details p53 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p53 protein. (region surrounding Leu369)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details p53Product Details anti-p53 Antibody Application Details Handling Images back to top
|Alternative Name||p53 (TP53) (TP53 Antibody Abstract)|
|Background||P53 is a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53|
|Molecular Weight||approx. 53, 43 kDa|
|Pathways||p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling|
Application DetailsProduct Details anti-p53 Antibody Target Details p53 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-p53 Antibody Target Details p53 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-p53 Antibody Target Details p53 Application Details Handling back to top