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|Application / Reactivity||Human|
|Dot Blot (DB)||1 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||5 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||12 Antibodies|
|Immunofluorescence (IF)||12 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||42 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||18 Antibodies|
|Immunoprecipitation (IP)||14 Antibodies|
|Western Blotting (WB)||152 Antibodies|
|Antigen||Peroxiredoxin 1 (PRDX1) Antibodies|
|Conjugate||This Peroxiredoxin 1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-Peroxiredoxin 1 AntibodyTarget Details Peroxiredoxin 1 Application Details Handling Images
|Characteristics||Monoclonal Antibody to detect peroxiredoxin I in human samples.|
|Purification||Protein G purified Antibody|
|Immunogen||Recombinant human protein purified from E.coli|
Target Details Peroxiredoxin 1Product Details anti-Peroxiredoxin 1 Antibody Application Details Handling Images back to top
|Alternative Name||Peroxiredoxin 1 (PRDX1 Antibody Abstract)|
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 - 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx I is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor alpha by regulating the intracellular concentrations of H2O2. Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive post mitotic motor neuron differentiation.
Synonyms: Peroxiredoxin 1, MSP23, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2, Thioredoxin peroxidase 2.
|Research Area||Cardiovascular, Atherosclerosis, Oxidative Stress|
|Pathways||p53 Signaling, EGFR Signaling Pathway|
Application DetailsProduct Details anti-Peroxiredoxin 1 Antibody Target Details Peroxiredoxin 1 Handling Images back to top
|Application Notes||Western blot: 2-4 μL/mL, IP: 1-2 μL, ICC: 20 μL/mL.|
Suggested positive control: IP analysis : HeLa cell lysate. IHC staining : HeLa cells
|Restrictions||For Research Use only|
HandlingProduct Details anti-Peroxiredoxin 1 Antibody Target Details Peroxiredoxin 1 Application Details Images back to top
|Buffer||100 μL of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Expiry Date||12 months|
ImagesProduct Details anti-Peroxiredoxin 1 Antibody Target Details Peroxiredoxin 1 Application Details Handling back to top