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Pig (Porcine) SERPINE1 ELISA Kit

Recommended SERPINE1 Product (supplied by: Log in to see )

Antigen
serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kits
  • B230326M24Rik
  • HsT1201
  • PAI
  • PAI-1
  • PAI-2
  • Pai1
  • PAI1
  • PAI1A
  • Pai1aa
  • PAI2
  • PI-7
  • PI7
  • Planh
  • Planh1
  • PLANH1
  • PLANH2
  • PN-1
  • RATPAI1A
  • SERPINE1
  • si:ch211-138a11.1
  • Spi4
Reactivity
Pig (Porcine)
Alternatives
Kits with alternative reactivity to:
35
18
16
13
10
8
5
4
4
3
2
2
1
Methode Type
Sandwich ELISA
Detection Range
0.2-200 ng/mL
Minimum Detection Limit
0.2 ng/mL
Application
ELISA
Supplier
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Catalog No. ABIN2010780
$ 572.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN2010729 Sandwich ELISA 0.05-50 ng/mL 0.05 ng/mL Log in to see 4
0.0012117105 ABIN368422 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.0012117105 ABIN512224 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 2.5-50 ng/mL 2.5 ng/mL Log in to see
0.0012117105 ABIN578899 Plasma, Biological Fluids Log in to see
0.0012117105 ABIN1565445 Sandwich ELISA Serum, Plasma, Biological Fluids Log in to see
0.0012117105 ABIN1565454 Sandwich ELISA Serum, Plasma, Biological Fluids Log in to see
0.0012117105 ABIN2950421 0.313-20 ng/mL 0.313 ng/mL Log in to see
0.0012117105 ABIN2537545 Serum, Plasma, Biological Fluids 80-4000 pg/mL 80 pg/mL Log in to see
0.0012117105 ABIN2101284 Competition ELISA Log in to see
0.0012117105 ABIN780357 Log in to see

Top referenced SERPINE1 ELISA Kit for ELISA

  • ABIN2010729 (4 Publications): Duburcq, Tournoys, Viviane, Hubert, Gmyr, Pattou, Jourdain: Impact of Obesity on Endotoxin-Induced Disseminated Intravascular Coagulation. in Shock (Augusta, Ga.) 2015 (PubMed)

  • Pig (Porcine) SERPINE1 ELISA Kit for Sandwich ELISA - ABIN2010780 (2 Publications): Thorgersen, Hellerud, Nielsen, Barratt-Due, Fure, Lindstad, Pharo, Fosse, Tønnessen, Johansen, Castellheim, Mollnes: CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs. in FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 (PubMed)

Similar SERPINE1 ELISA Kits

Application / Reactivity Pig (Porcine)
ELISA 11 ELISA Kits

General

Antigen serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kits
Reactivity Pig (Porcine)
Kits with alternative reactivity to:
(35), (18), (16), (13), (10), (8), (5), (4), (4), (3), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 0.2-200 ng/mL
Minimum Detection Limit 0.2 ng/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details SERPINE1 ELISA Kit

Target details Application Details Handling References for SERPINE1 Kit (ABIN2010780) Images
Purpose The sensitive quantitative measurement of total porcine PAI-1 tPA complex antigen in plasma, serum, cell culture media, or tissue extract samples is easily performed with this 96 well strip format ELISA kit.
Detection Method Colorimetric
Sensitivity 0.178 ng/mL
Components All reagents and standards are provided in this kit.

Target details

Product Details SERPINE1 ELISA Kit Application Details Handling References for SERPINE1 Kit (ABIN2010780) Images back to top
Antigen
Alternative Name PAI-1 (SERPINE1 ELISA Kit Abstract)
Background Tissue-type plasminogen activator (tPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system and forms a 1:1 covalent complex with tPA and uPA.
Gene ID 396945
UniProt P79335
Pathways p53 Signaling

Application Details

Product Details SERPINE1 ELISA Kit Target details Handling References for SERPINE1 Kit (ABIN2010780) Images back to top
Plate Strips
Protocol Concentration of PAI-1 tPA complex was found to be 10.2 ng/ml in normal porcine plasma and 37.7 ng/ml in normal porcine serum. The assay measures PAI-1 tPA complex in the 0.2-200 ng/ml range. Free tPA and PAI-1 will not be detected by this assay. Plasma and serum samples should be applied directly to the plate without dilution for best results. Porcine tPA will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 tPA complex in the samples. A standard calibration curve is prepared in PAI-1 depleted plasma using dilutions of purified PAI-1 tPA complex and is measured along with the test samples.
Restrictions For Research Use only

Handling

Product Details SERPINE1 ELISA Kit Target details Application Details References for SERPINE1 Kit (ABIN2010780) Images back to top
Storage 4 °C

References for SERPINE1 Kit (ABIN2010780)

Product Details SERPINE1 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Barratt-Due, Thorgersen, Egge, Pischke, Sokolov, Hellerud, Lindstad, Pharo, Bongoni, Rieben, Nunn, Scott, Mollnes: "Combined inhibition of complement C5 and CD14 markedly attenuates inflammation, thrombogenicity, and hemodynamic changes in porcine sepsis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 2, pp. 819-27, 2013

Thorgersen, Hellerud, Nielsen, Barratt-Due, Fure, Lindstad, Pharo, Fosse, Tønnessen, Johansen, Castellheim, Mollnes: "CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 3, pp. 712-22, 2010

Images

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Supplier Images
ELISA image for SERPINE1 ELISA Kit (serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1) (ABIN2010780) serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kit