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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||9 Antibodies||9 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||6 Antibodies||8 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||14 Antibodies||16 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||13 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Western Blotting (WB)||32 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Antigen||TRAF Family Member-Associated NFKB Activator (TANK) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TANK antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-TANK AntibodyTarget Details TANK Application Details Handling Images
|Specificity||This antibody detects endogenous levels of TANK protein. (region surrounding Phe194)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography using epitope-specific immunogen.|
Target Details TANKProduct Details anti-TANK Antibody Application Details Handling Images back to top
|Alternative Name||TANK / ITRAF (TANK Antibody Abstract)|
|Background||The tumor necrosis factor (TNF) receptor superfamily is composed of several type I integral membrane glycoproteins that exhibit homology in their cystine- rich extracellular domains. Members of this family include TNF-RI and -RII, FAS, OX40, CD27, CD30 and CD40. Ligands for these receptors can be small, secreted proteins such as TNF, or type II integral membrane proteins, such as the CD40 ligand, CD40L. While the signal transduction mechanism of the TNF receptor superfamily is poorly understood, stimulation of cells with either TNF or soluble CD40L has been shown to induce the nuclear translocation of NFκB. Members of the TRAF family associate with activated TNF-R and CD40 and have been implicated in this process. The discovery of a novel protein, designated TANK, has shed light on the means by which TRAF activation of NFkB occurs. TANK is not only capable of binding to all three TRAFs, but also of synergizing with TRAF2 to activate the NFκB signaling cascade. TANK contains a regulatory carboxy terminal domain that maintains its inactivity in unstimulated cells. Upon its association with TRAF2, the inhibitory effect of this domain is overcome.Synonyms: I-TRAF, TRAF family member-associated NF-kappa-B activator, TRAF-interacting protein, TRAF2|
|Molecular Weight||approx. 48 kDa|
|Pathways||p53 Signaling, TLR Signaling, Activation of Innate immune Response|
Application DetailsProduct Details anti-TANK Antibody Target Details TANK Handling Images back to top
ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TANK Antibody Target Details TANK Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-TANK Antibody Target Details TANK Application Details Handling back to top