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anti-Human TANK antibody for Enzyme Immunoassay

Recommended TANK Antibody (supplied by: Log in to see )

Antigen
TRAF Family Member-Associated NFKB Activator (TANK) Antibodies
  • C86182
  • E430026L09Rik
  • I-TRAF
  • ITRAF
  • TRAF2
  • TRAF family member-associated NFKB activator
  • TRAF family member-associated Nf-kappa B activator
  • TANK
  • Tank
Epitope
N-Term
20
19
14
12
10
5
4
3
2
2
1
1
1
1
1
Reactivity
Human
118
47
43
10
5
4
4
4
3
3
2
2
Host
Rabbit
112
3
2
1
1
Clonality
Polyclonal
Conjugate
This TANK antibody is un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
105
43
35
24
16
14
13
13
9
3
2
2
1
Supplier
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Catalog No. ABIN500869
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN500867 EIA IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 5
1 ABIN272299 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-TANK antibody for Enzyme Immunoassay

Similar anti-TANK Antibodies

Application / Reactivity Human
ELISA 35 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Western Blotting (WB) 105 Antibodies

General

Antigen TRAF Family Member-Associated NFKB Activator (TANK) Antibodies
Epitope N-Term
(20), (19), (14), (12), (10), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(118), (47), (43), (10), (5), (4), (4), (4), (3), (3), (2), (2)
Host Rabbit
(112), (3), (2), (1), (1)
Clonality
Polyclonal
Conjugate This TANK antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(105), (43), (35), (24), (16), (14), (13), (13), (9), (3), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-TANK Antibody

Target Details TANK Application Details Handling References for anti-TANK Antibody (ABIN500869) Images
Specificity This antibody detects TANK / ITRAF at N-term.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Peptide affinity chromatography
Immunogen TANK antibody was raised against a 14 amino acid peptide from near the amino terminus of human TANK.
Isotype IgG

Target Details TANK

Product Details anti-TANK Antibody Application Details Handling References for anti-TANK Antibody (ABIN500869) Images back to top
Antigen
Alternative Name TANK / ITRAF (TANK Antibody Abstract)
Background TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-kappaB. This activation of NF-kappaB occurs through an association with the kinases IKKepsilon and TBK1. More recently, it was shown that these proteins can then form a complex with NEMO, a protein that regulates the activity of the IkappaB complex. This suggests that in addition to the possibility that TBK1 and IKKepsilon activate the IKKs, the association with the IKK complex may help these kinases modulate other functions, such as the transactivation potential of NF-kappaB proteins. At least two isoforms of TANK are known to exist.Synonyms: I-TRAF, TRAF family member-associated NF-kappa-B activator, TRAF-interacting protein, TRAF2
Gene ID 10010
NCBI Accession NP_004171
Pathways p53 Signaling, TLR Signaling, Activation of Innate immune Response

Application Details

Product Details anti-TANK Antibody Target Details TANK Handling References for anti-TANK Antibody (ABIN500869) Images back to top
Application Notes ELISA. Western blot: 0.5 - 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-TANK Antibody Target Details TANK Application Details References for anti-TANK Antibody (ABIN500869) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-TANK Antibody (ABIN500869)

Product Details anti-TANK Antibody Target Details TANK Application Details Handling Images back to top
Background publications

Rao, Kadesch: "The intracellular form of notch blocks transforming growth factor beta-mediated growth arrest in Mv1Lu epithelial cells." in: Molecular and cellular biology, Vol. 23, Issue 18, pp. 6694-701, 2003

Chariot, Leonardi, Muller, Bonif, Brown, Siebenlist: "Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 37029-36, 2002

Pomerantz, Baltimore: "NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase." in: The EMBO journal, Vol. 18, Issue 23, pp. 6694-704, 2000

Cheng, Baltimore: "TANK, a co-inducer with TRAF2 of TNF- and CD 40L-mediated NF-kappaB activation." in: Genes & development, Vol. 10, Issue 8, pp. 963-73, 1996

Rothe, Xiong, Shu, Williamson, Goddard, Goeddel: "I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 16, pp. 8241-6, 1996

Images

Product Details anti-TANK Antibody Target Details TANK Application Details Handling References for anti-TANK Antibody (ABIN500869) back to top
Supplier Images
Immunofluorescence (IF) image for anti-TANK antibody (TRAF Family Member-Associated NFKB Activator) (N-Term) (ABIN500869) Immunocytochemistry of TANK in Daudi cells with this product at 2.5 μg/ml.
Western Blotting (WB) image for anti-TANK antibody (TRAF Family Member-Associated NFKB Activator) (N-Term) (ABIN500869) Western blot analysis of TANK in Daudi cell lysate with this product at (A) 0.5 and (...