TANK antibody (C-Term)

Catalog No. ABIN500867

The Rabbit Polyclonal anti-TANK antibody is suitable to detect TANK in samples from Human, Mouse and Rat. It has been validated for WB, IF, IHC (p) and EIA.

Quick Overview for TANK antibody (C-Term) (ABIN500867)

Target: TANK (TRAF Family Member-Associated NFKB Activator (TANK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TANK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-TANK Antibody

Binding Specificity: C-Term

Specificity: This antibody detects TANK / ITRAF at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Peptide Affinity Chromatography

Immunogen: 14 amino acid peptide from near the carboxy terminus of Human TANK.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TANK

Target: TANK (TRAF Family Member-Associated NFKB Activator (TANK))

Alternative Name: TANK / ITRAF

Background: TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-kappaB. This activation of NF-kappaB occurs through an association with the kinases IKKepsilon and TBK1. More recently, it was shown that these proteins can then form a complex with NEMO, a protein that regulates the activity of the IkappaB complex. This suggests that in addition to the possibility that TBK1 and IKKepsilon activate the IKKs, the association with the IKK complex may help these kinases modulate other functions, such as the transactivation potential of NF-kappaB proteins. At least two isoforms of TANK are known to exist.Synonyms: I-TRAF, TRAF family member-associated NF-kappa-B activator, TRAF-interacting protein, TRAF2

Gene ID: 10010

NCBI Accession: NP_004171

Pathways: p53 Signaling, TLR Signaling, Activation of Innate immune Response