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Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2) (AA 18-517) Peptide

ALDH2 Reactivity: Human Host: Escherichia coli (E. coli) SDS Dithiothreitol (DTT)
Catalog No. ABIN935020
  • Target See all ALDH2 products
    ALDH2 (Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2))
    Peptide Type
    Recombinant
    Biological Activity
    Active
    Protein Region
    AA 18-517
    Origin
    Human
    Source
    • 3
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Sequence
    MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
    Characteristics
    Purified recombinant Human ALDH2 protein
    Expression System: E.coli
    Bioactivity: Specific activity is < 0.2 units/mL, and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 uM of acetaldehyde to acetic acid per minute at pH 8.0 at 25 °C in the presence of beta-NAD, potassium and thiols.
    Molecular weight on SDS-PAGE will appear higher.
    Purity
    > 90 % pure
    Endotoxin Level
    < 1.0 EU per µg of protein (determined by LAL method)
  • Application Notes
    ALDH2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Assay Procedure
    1. Prepare a 3 mL reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA.
      2. Equilibrate to 25 °C and monitor at A340 nm until value is constant using a spectrophotometer.
      3. Add 5 µg of recombinant ALDH2 into reaction mixture and mix immediately.
      4. Record the increase at A340 nm for 5 minutes
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 0 mM DTT, 0 mM EDTA, and 10 % glycerol.
    Preservative
    Dithiothreitol (DTT)
    Precaution of Use
    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    RT/-20 °C
    Storage Comment
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target
    ALDH2 (Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2))
    Synonyms
    aldh2 Peptide, aldh2a Peptide, zgc:63786 Peptide, MGC80785 Peptide, aldh-e2 Peptide, aldhi Peptide, aldm Peptide, rf2b Peptide, ALDH-E2 Peptide, ALDHI Peptide, ALDM Peptide, Ahd-5 Peptide, Ahd5 Peptide, aldehyde dehydrogenase 2 family (mitochondrial), tandem duplicate 1 Peptide, aldehyde dehydrogenase 2 family (mitochondrial) L homeolog Peptide, aldehyde dehydrogenase 2 family (mitochondrial) Peptide, aldehyde dehydrogenase 2 Peptide, aldehyde dehydrogenase Peptide, aldehyde dehydrogenase (NAD(+)) Peptide, hypothetical protein Peptide, aldehyde dehydrogenase 2, mitochondrial Peptide, aldh2.1 Peptide, aldh2.L Peptide, aldh2 Peptide, ALDH2 Peptide, RHA1_RS43090 Peptide, aldH2 Peptide, OE_RS03015 Peptide, Aldh2 Peptide
    Background
    ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant human ALDH2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: Aldehyde dehydrogenase mitochondrial protein, ALDH E2 protein, mitochondrial Acetaldehyde dehydrogenase 2 protein, ALDH-2, Aldehyde dehydrogenase protein, ALDH 2 protein, ALDH 2 protein, ALDH-2 protein, ALDM protein, MGC1806 protein, ALDH 2, Aldehyde dehydrogenase 2 family protein, Mitochondrial aldehyde dehydrogenase 2 protein, ALDH class 2 protein, ALDH2, ALDHI protein, Nucleus encoded mitochondrial aldehyde dehydrogenase 2., Aldehyde dehydrogenase 2 family (mitochondrial) protein, ALDH-E2 protein, Liver mitochondrial ALDH protein
    Molecular Weight
    54.5 kDa (501 AA)
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