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anti-Arabidopsis thaliana AKT antibody for Western Blotting

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Reactivity
Arabidopsis thaliana
1065
473
381
43
41
31
30
20
17
15
10
6
5
4
2
2
1
1
Host
Rabbit
877
208
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
40
33
33
32
27
25
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
879
516
414
201
109
107
107
71
42
36
24
12
11
6
3
3
3
3
1
1
1
1
1
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Catalog No. ABIN488530
$ 497.81
Plus shipping costs $45.00

Top referenced anti-AKT antibody for Western Blotting

Similar anti-AKT Antibodies

Application / Reactivity Arabidopsis thaliana
Western Blotting (WB) 1 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Reactivity Arabidopsis thaliana
(1065), (473), (381), (43), (41), (31), (30), (20), (17), (15), (10), (6), (5), (4), (2), (2), (1), (1)
Host Rabbit
(877), (208), (14), (4), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(40), (33), (33), (32), (27), (25), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(879), (516), (414), (201), (109), (107), (107), (71), (42), (36), (24), (12), (11), (6), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN488530) Images
Predicted Reactivity Arabidopsis thaliana
Characteristics Expected / apparent Molecular Weight of the Antigene: 96.9 kDa
Purification serum
Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN488530) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background AGI Code: At2g26650
AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
Molecular Weight 96.9 kDa
UniProt Q38998
Research Area Cancer, Cell Cycle, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN488530) Images back to top
Application Notes Recommended Dilution: 1 : 50 with 125I (WB).
Comment

In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN488530) Images back to top
Format Lyophilized
Reconstitution For reconstitution add 100 µL of sterile water.
Handling Advice Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Storage -20 °C

References for anti-AKT Antibody (ABIN488530)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Honsbein, Sokolovski, Grefen, Campanoni, Pratelli, Paneque, Chen, Johansson, Blatt: "A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis." in: The Plant cell, Vol. 21, Issue 9, pp. 2859-77, 2009