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anti-Chicken AKT antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Epitope
C-Term
142
113
88
85
54
39
38
38
26
25
21
20
18
16
15
15
14
13
13
11
11
10
8
7
6
6
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Chicken
1064
472
380
42
41
31
30
20
17
15
10
6
5
4
2
2
1
1
1
Host
Rabbit
877
208
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
40
33
33
32
27
25
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
879
515
414
201
108
107
107
71
42
36
24
12
11
6
3
3
2
2
1
1
1
1
1
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Catalog No. ABIN1043683
$ 135.00
Plus shipping costs $45.00 and $21.00 dry ice

Top referenced anti-AKT antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Similar anti-AKT Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 144 Antibodies
Enzyme Immunoassay (EIA) 21 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 84 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 205 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 39 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies
Western Blotting (WB) 330 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope C-Term
(142), (113), (88), (85), (54), (39), (38), (38), (26), (25), (21), (20), (18), (16), (15), (15), (14), (13), (13), (11), (11), (10), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Chicken
(1064), (472), (380), (42), (41), (31), (30), (20), (17), (15), (10), (6), (5), (4), (2), (2), (1), (1), (1)
Host Rabbit
(877), (208), (14), (4), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(40), (33), (33), (32), (27), (25), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Fluorescence Microscopy (FM), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(879), (515), (414), (201), (108), (107), (107), (71), (42), (36), (24), (12), (11), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1043683) Images
Specificity AKT Antibody detects AKT which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Characteristics AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Sterility Sterile filtered
Immunogen AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type: Peptide

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN1043683) Images back to top
Antigen
Alternative Name AKT (AKT1 Antibody Abstract)
Background AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Gene ID 207, 62241011
UniProt P31749
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN1043683) Images back to top
Application Notes AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Comment

Gene Name: AKT1

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN1043683) Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-AKT Antibody (ABIN1043683)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Matsuda, Takano, Kageyama, Hatakeyama, Shakunaga, Kitajima, Yamazaki, Hattori: "Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock." in: Cardiovascular research, Vol. 76, Issue 1, pp. 132-40, 2007

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1043683) back to top
Supplier Images
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (C-Term) (ABIN1043683) Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody. Tissue: neonatal rat cardi...
Immunohistochemistry (IHC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (C-Term) (ABIN1043683) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (Image 2)
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (C-Term) (ABIN1043683) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (Image 3)
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (C-Term) (ABIN1043683) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (Image 4)