anti-Chicken AKT antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
C-Term
188
122
85
65
56
39
32
30
28
22
21
21
18
18
17
14
13
12
12
11
11
10
10
9
9
9
8
7
7
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Chicken
1136
636
493
63
57
56
46
35
33
30
18
8
8
5
5
5
3
3
3
2
1
1
1
1
Host
Rabbit
855
323
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
48
38
35
35
26
25
12
12
7
7
6
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
Fluorescence Microscopy (FM), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
964
526
435
220
159
149
115
67
56
33
26
15
11
11
4
3
3
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN1043683
$ 135.00
Plus shipping costs $45.00 and $21.00 dry ice

Top referenced anti-AKT antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

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Application / Reactivity Rat (Rattus)
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ELISA 171 Antibodies
Enzyme Immunoassay (EIA) 20 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 49 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 107 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 219 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 91 Antibodies
Immunoprecipitation (IP) 58 Antibodies
Intracellular Staining (ICS) 3 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope C-Term
(188), (122), (85), (65), (56), (39), (32), (30), (28), (22), (21), (21), (18), (18), (17), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Chicken
(1136), (636), (493), (63), (57), (56), (46), (35), (33), (30), (18), (8), (8), (5), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(855), (323), (11), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(48), (38), (35), (35), (26), (25), (12), (12), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Fluorescence Microscopy (FM), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(964), (526), (435), (220), (159), (149), (115), (67), (56), (33), (26), (15), (11), (11), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN1043683) Images
Specificity AKT Antibody detects AKT which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Characteristics AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Sterility Sterile filtered
Immunogen AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type: Peptide

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN1043683) Images back to top
Antigen
Alternative Name AKT (AKT1 Antibody Abstract)
Background AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Gene ID 207, 62241011
UniProt P31749
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling ProductDetails: References for anti-AKT antibody (ABIN1043683) Images back to top
Application Notes AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Comment

Gene Name: AKT1

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details ProductDetails: References for anti-AKT antibody (ABIN1043683) Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

ProductDetails: References for anti-AKT antibody (ABIN1043683)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Matsuda, Takano, Kageyama, Hatakeyama, Shakunaga, Kitajima, Yamazaki, Hattori: "Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock." in: Cardiovascular research, Vol. 76, Issue 1, pp. 132-40, 2007

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN1043683) back to top
Supplier Images
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (C-Term) (ABIN1043683) Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody. Tissue: neonatal rat cardi...
Immunohistochemistry (IHC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (C-Term) (ABIN1043683) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (Image 2)
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (C-Term) (ABIN1043683) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (Image 3)
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (C-Term) (ABIN1043683) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (Image 4)