anti-Dog (Canine) AKT antibody for ELISA

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Reactivity
Dog (Canine), Human, Mouse (Murine)
1221
646
503
63
58
56
45
35
33
30
18
8
8
6
5
5
3
3
3
2
1
1
1
1
1
Host
Rabbit
883
381
12
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
54
41
39
38
28
25
15
15
10
10
8
6
6
6
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
ELISA, Western Blotting (WB)
1040
551
455
253
183
175
120
82
67
33
26
15
13
11
11
4
3
3
3
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2463692
$ 433.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2463693 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN609140 IF IHC (p) IP ELISA WB Rabbit AA 404-418 Log in to see Polyclonal

Similar anti-AKT Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies 12 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Simple Western (SimWes) 2 Antibodies 2 Antibodies
Western Blotting (WB) 982 Antibodies 553 Antibodies 42 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Luminex Assay (LMNX) 3 Antibodies
Intracellular Staining (ICS) 11 Antibodies 11 Antibodies 3 Antibodies
Immunoprecipitation (IP) 107 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine)
(1221), (646), (503), (63), (58), (56), (45), (35), (33), (30), (18), (8), (8), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(883), (381), (12), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(54), (41), (39), (38), (28), (25), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(1040), (551), (455), (253), (183), (175), (120), (82), (67), (33), (26), (15), (13), (11), (11), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Purification Antibody is purified by peptide affinity chromatography method.
Immunogen Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AKT1.

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Molecular Weight 56 kDa
Gene ID 207
NCBI Accession NP_005154
UniProt P31749
Research Area Cancer, Cell Cycle, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes AKT1 antibody can be used for detection of AKT1 by ELISA at 1:312500. AKT1 antibody can be used for detection of AKT1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Lyophilized
Reconstitution Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration 1 mg/mL
Buffer Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice As with any antibody avoid repeat freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment For short periods of storage (days) store at 4 °C. For longer periods of storage, store AKT1 antibody at -20 °C.

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (ABIN2463692) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody