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anti-Fly (Calliphora) AKT antibody for Western Blotting

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
AA 1-480
186
123
84
64
54
39
32
30
26
21
21
18
17
15
15
14
13
12
12
11
10
10
10
9
9
9
8
7
6
6
6
6
6
6
5
5
5
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus)
1100
615
475
61
58
55
45
35
33
30
18
8
8
5
5
5
3
3
2
1
1
1
1
1
Host
Mouse
842
301
11
4
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
46
38
35
35
26
25
10
10
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
942
517
432
217
146
141
115
67
52
32
26
15
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN968227
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2688841 IF WB Mouse IgG2a AA 1-480 Log in to see 2-PKBa-Akt

Top referenced anti-AKT antibody for Western Blotting

Similar anti-AKT Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Fly (Calliphora)
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies 12 Antibodies 11 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Simple Western (SimWes) 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 887 Antibodies 530 Antibodies 412 Antibodies 42 Antibodies 2 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies 1 Antibodies 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 1-480
(186), (123), (84), (64), (54), (39), (32), (30), (26), (21), (21), (18), (17), (15), (15), (14), (13), (12), (12), (11), (10), (10), (10), (9), (9), (9), (8), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus)
(1100), (615), (475), (61), (58), (55), (45), (35), (33), (30), (18), (8), (8), (5), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(842), (301), (11), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(46), (38), (35), (35), (26), (25), (10), (10), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(942), (517), (432), (217), (146), (141), (115), (67), (52), (32), (26), (15), (11), (11), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN968227) Images
Cross-Reactivity Dog (Canine), Mouse (Murine), Rat (Rattus), Fruit Fly (Drosophila melanogaster)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human PKBalpha/Akt1 aa. 1-480
Clone 2-PKBa-Akt
Isotype IgG2a

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN968227) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Background Protein Kinase B (PKB), also known as Rac (Related to the A and C kinases) Protein Kinase, shows 73% and 68% to Protein Kinase C (PKC) and Protein Kinase A (PKA), respectively. It was also identified as the product of the v-akt oncogene from T-cell lymphomas. Akt/PKB mRNA has been reported to be expressed in a wide variety of cell lines and has been detected in porcine brain, heart, liver, kidney, muscle, and ovary. Rac phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt/PKB is activated in response to growth factors through the activation of PI3-kinase and Ras. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate which may induce the membrane translocation of Akt/PKB coincident with its phosphorylation and activation. Cellular stress leading to the activation of the p38 MAP kinase also induces Akt/PKB activation, indicating a multiplicity of signaling pathways that activate Akt/PKB. Upon activation, Akt/PKB associates with some members of the PKC family of kinases, such as PKCdelta and PKCzeta. Downstream of Akt/PKB, GSK3 is thus far the only identified substrate for Akt/PKB phosphorylated in vivo. This antibody is routinely tested by western blot analysis.
Synonyms: PKBalpha, RAC-PK
Molecular Weight 59 kDa
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN968227) Images back to top
Comment

Related Products: ABIN968551, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN968227) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-AKT Antibody (ABIN968227)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Marte, Downward: "PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond." in: Trends in biochemical sciences, Vol. 22, Issue 9, pp. 355-8, 1997

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN968227) back to top
Supplier Images
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 1-480) (ABIN968227) Western blot analysis of Akt on a HCT-8 (human colorectal adenocarcinoma) cell lysate...
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 1-480) (ABIN968227) Immunofluorescence staining on WI-38 cells (human lung fibroblasts, ATCC CCL-75).
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 1-480) (ABIN968227) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody (Image 3)