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anti-Human AKT antibody for Blocking Reagent

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Reactivity
Human
1142
532
398
43
41
34
33
32
20
17
10
6
5
4
2
2
2
1
1
1
Host
Rabbit
891
291
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
45
36
35
34
27
25
10
9
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
958
547
432
216
129
121
111
71
57
36
24
12
11
6
3
3
3
3
1
1
1
1
1
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Catalog No. ABIN2666122
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Top referenced anti-AKT antibody for Blocking Reagent

Similar anti-AKT Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
Dot Blot (DB) 71 Antibodies
ELISA 512 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 36 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 125 Antibodies
Fluorescence Microscopy (FM) 3 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 59 Antibodies
Immunofluorescence (IF) 216 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 414 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 122 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Reactivity Human
(1142), (532), (398), (43), (41), (34), (33), (32), (20), (17), (10), (6), (5), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(891), (291), (14), (4), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This AKT antibody is un-conjugated
(45), (36), (35), (34), (27), (25), (10), (9), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(958), (547), (432), (216), (129), (121), (111), (71), (57), (36), (24), (12), (11), (6), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN2666122) Images
Purification The antibody was purified by antigen-affinity chromatography.
Clone Poly6034
Isotype IgG

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN2666122) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Background Akt (also known as protein kinase B alpha) is a 60 kD serine/threonine specific kinase containing a pleckstrin domain. This kinase is ubiquitously expressed and translocates to the membrane upon activation. Akt phosphorylates substrates in both the cytoplasm and the nucleus. Akt exists as a catalytically inactive multimeric complex until phosphatidyl-3,4,5-triphosphate (PIP3) is generated as a result of cell signaling. Elevated PIP3 results in the partial activation of Akt after phosphorylation by phosphoinositide-dependent protein kinase 1 complex, Akt becomes fully activated only after autophosphorylation. Akt is a potent inhibitor of apoptosis and has been implicated as a factor in tumorigenesis and progression. Survival factors that suppress apoptosis activate Akt which phosphorylates and inactivates components of the apoptotic machinery. Akt can also be activated by TNF, IL-1, PDGF, and insulin like growth factor 1. Akt interacts through its pleckstrin homology domain with the second messengers phosphatidylinositol-(3,4,5)-trisphosphate (PtdIns(3,4,5)P[3]) and PtdIns(3,4). Akt has also been shown to interact with PED/PEA, TCL-1, Nur77, inosine-5'-monophosphate dehydrogenase, and PKC-ζ. The Poly6034 antibody has been shown to be useful for Western blotting of human Akt.
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN2666122) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN2666122) Images back to top
Format Liquid
Buffer phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Upon receipt, store frozen at -20°C.

References for anti-AKT Antibody (ABIN2666122)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Brunet, Bonni, Zigmond, Lin, Juo, Hu, Anderson, Arden, Blenis, Greenberg: "Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor." in: Cell, Vol. 96, Issue 6, pp. 857-68, 1999

Franke, Yang, Chan, Datta, Kazlauskas, Morrison, Kaplan, Tsichlis: "The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase." in: Cell, Vol. 81, Issue 5, pp. 727-36, 1995

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN2666122) back to top
Supplier Images
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (ABIN2666122) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (ABIN2666122) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody (Image 2)