anti-Human AKT antibody for Dot Blot

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
pThr450
208
123
103
65
56
40
39
30
28
22
21
21
18
18
17
14
13
12
12
11
11
10
10
9
9
9
8
7
7
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1221
647
503
63
58
56
46
35
33
30
18
8
8
6
5
5
3
3
3
2
1
1
1
1
1
Host
Rabbit
883
381
12
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
54
41
39
38
28
25
15
15
10
10
8
6
6
6
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Dot Blot (DB)
1041
552
455
253
183
175
120
82
66
33
26
15
13
11
11
4
3
3
3
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN650890
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
29.191875 ABIN650880 DB IF Rabbit Ig pThr308 Log in to see Polyclonal 1
29.191875 ABIN389509 DB Rabbit Ig pSer129 Log in to see Polyclonal 4
29.191875 ABIN389819 DB WB Rabbit Ig pSer473 Log in to see Polyclonal 4
29.191875 ABIN3029462 DB ELISA Rabbit Ig Fraction pSer129 Log in to see Polyclonal
29.191875 ABIN3029463 DB ELISA Rabbit Ig Fraction pThr450 Log in to see Polyclonal
29.191875 ABIN3029455 DB ELISA WB Rabbit Ig Fraction pSer473 Log in to see Polyclonal
29.191875 ABIN3029461 DB ELISA Rabbit Ig Fraction pSer124 Log in to see Polyclonal
29.191875 ABIN3029454 DB IF ELISA Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN5552139 DB EIA Rabbit Ig Fraction pSer473 Log in to see Polyclonal
1 ABIN2615412 DB ELISA Rabbit IgG pThr450 Log in to see Polyclonal
1 ABIN2615415 DB ELISA IF Rabbit IgG Thr308 Log in to see Polyclonal
1 ABIN1953795 DB ELISA APC Rabbit IgG Ser473 Log in to see Polyclonal
1 ABIN1956237 DB ELISA FITC Rabbit IgG Ser473 Log in to see Polyclonal
1 ABIN1952923 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Ser473 Log in to see Polyclonal
1 ABIN1955072 DB ELISA Biotin Rabbit IgG Ser473 Log in to see Polyclonal
1 ABIN2615420 DB ELISA Rabbit IgG pSer473 Log in to see Polyclonal
1 ABIN2198457 DB ELISA FITC Rabbit IgG pSer473 Log in to see Polyclonal
1 ABIN2198458 DB ELISA HRP Rabbit IgG pSer473 Log in to see Polyclonal
1 ABIN2198459 DB ELISA WB PE Rabbit IgG pSer473 Log in to see Polyclonal
1 ABIN2198484 DB IF ELISA APC Rabbit IgG pThr308 Log in to see Polyclonal

Top referenced anti-AKT antibody for Dot Blot

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Application / Reactivity Human
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Dot Blot (DB) 67 Antibodies
ELISA 513 Antibodies
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Enzyme Immunoassay (EIA) 33 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 178 Antibodies
Fluorescence Microscopy (FM) 13 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 82 Antibodies
Immunofluorescence (IF) 250 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies
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Immunohistochemistry (IHC) 418 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pThr450
(208), (123), (103), (65), (56), (40), (39), (30), (28), (22), (21), (21), (18), (18), (17), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1221), (647), (503), (63), (58), (56), (46), (35), (33), (30), (18), (8), (8), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(883), (381), (12), (4), (2), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This AKT antibody is un-conjugated
(54), (41), (39), (38), (28), (25), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Dot Blot (DB)
(1041), (552), (455), (253), (183), (175), (120), (82), (66), (33), (26), (15), (13), (11), (11), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN650890) Images
Specificity This Phospho-AKT1-T450 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide surrounding T450 of human AKT1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This AKT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T450 of human AKT1.
Clone RB29255
Isotype Ig

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN650890) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Synonyms: RAC-alpha serine/threonine-protein kinase,AKT1,PKB, RAC
Molecular Weight 55686 DA
Gene ID 207
NCBI Accession NP_001014431, NP_001014432, NP_005154
UniProt P31749
Research Area Translation Factors, Protein Modifications, Signaling, Neurology, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cancer, Chromatin, Metabolism
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling ProductDetails: References for anti-AKT antibody (ABIN650890) Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details ProductDetails: References for anti-AKT antibody (ABIN650890) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-AKT antibody (ABIN650890)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Chen, Gomes, Monteiro, Wong, Wu, Ng, Karadedou, Millour, Ip, Cheung, Sunters, Chan, Lam, Khoo: "Constitutively nuclear FOXO3a localization predicts poor survival and promotes Akt phosphorylation in breast cancer." in: PLoS ONE, Vol. 5, Issue 8, pp. e12293, 2010

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN650890) back to top
Supplier Images
Dot Blot (DB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr450) (ABIN650890) Dot blot analysis of anti-Phospho-AKT1-T450 Antibody Phospho-specific Pab (ABIN650890...