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anti-Human AKT antibody for Dot Blot

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Epitope
pThr450
186
136
90
88
53
39
38
38
26
25
21
20
18
16
16
15
15
14
13
13
11
11
10
8
7
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1138
529
396
43
41
34
33
32
20
17
10
6
5
4
2
2
2
1
1
1
Host
Rabbit
888
290
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
44
36
35
34
27
25
10
9
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Dot Blot (DB)
955
546
431
215
129
119
112
70
56
36
24
12
11
6
3
3
3
3
1
1
1
1
1
1
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Catalog No. ABIN650890
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.61607844 ABIN650880 DB IF Rabbit Ig pThr308 Log in to see Polyclonal 1
0.61607844 ABIN389509 DB Rabbit Ig pSer129 Log in to see Polyclonal 4
0.61607844 ABIN389819 DB WB Rabbit Ig pSer473 Log in to see Polyclonal 4
0.61607844 ABIN2159028 DB Rabbit Ig Fraction pSer129 Log in to see Polyclonal
0.61607844 ABIN2157654 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
0.61607844 ABIN2157655 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
0.61607844 ABIN2157653 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
0.0008871423 ABIN358371 DB EIA Rabbit Ig pSer473 Log in to see Polyclonal 5
0.0008871423 ABIN1952923 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Ser473 Log in to see Polyclonal
0.0008871423 ABIN1953099 DB ICC ELISA Alkaline Phosphatase (AP) Rabbit IgG Thr308 Log in to see Polyclonal
0.0008871423 ABIN1953116 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Thr450, pThr450 Log in to see Polyclonal
0.0008871423 ABIN1953795 DB ELISA APC Rabbit IgG Ser473 Log in to see Polyclonal
0.0008871423 ABIN1953971 DB ICC ELISA APC Rabbit IgG Thr308 Log in to see Polyclonal
0.0008871423 ABIN1953988 DB ELISA APC Rabbit IgG Thr450, pThr450 Log in to see Polyclonal
0.0008871423 ABIN1956237 DB ELISA FITC Rabbit IgG Ser473 Log in to see Polyclonal
0.0008871423 ABIN1956408 DB ICC ELISA FITC Rabbit IgG Thr308 Log in to see Polyclonal
0.0008871423 ABIN1956425 DB ELISA FITC Rabbit IgG Thr450, pThr450 Log in to see Polyclonal
0.0008871423 ABIN1955072 DB ELISA Biotin Rabbit IgG Ser473 Log in to see Polyclonal
0.0008871423 ABIN1955232 DB ICC ELISA Biotin Rabbit IgG Thr308 Log in to see Polyclonal
0.0008871423 ABIN1955248 DB ELISA Biotin Rabbit IgG Thr450, pThr450 Log in to see Polyclonal

Top referenced anti-AKT antibody for Dot Blot

Similar anti-AKT Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
Dot Blot (DB) 71 Antibodies
ELISA 512 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 36 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 124 Antibodies
Fluorescence Microscopy (FM) 3 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 59 Antibodies
Immunofluorescence (IF) 216 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 414 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 122 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pThr450
(186), (136), (90), (88), (53), (39), (38), (38), (26), (25), (21), (20), (18), (16), (16), (15), (15), (14), (13), (13), (11), (11), (10), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1138), (529), (396), (43), (41), (34), (33), (32), (20), (17), (10), (6), (5), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(888), (290), (14), (4), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This AKT antibody is un-conjugated
(44), (36), (35), (34), (27), (25), (10), (9), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB)
(955), (546), (431), (215), (129), (119), (112), (70), (56), (36), (24), (12), (11), (6), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN650890) Images
Specificity This Phospho-AKT1-T450 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide surrounding T450 of human AKT1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This AKT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T450 of human AKT1.
Clone RB29255
Isotype Ig

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN650890) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Synonyms: RAC-alpha serine/threonine-protein kinase,AKT1,PKB, RAC
Molecular Weight 55686 DA
Gene ID 207
NCBI Accession NP_001014431, NP_001014432, NP_005154
UniProt P31749
Research Area Translation Factors, Protein Modifications, Signaling, Neurology, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cancer, Chromatin, Metabolism
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN650890) Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN650890) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-AKT Antibody (ABIN650890)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Chen, Gomes, Monteiro, Wong, Wu, Ng, Karadedou, Millour, Ip, Cheung, Sunters, Chan, Lam, Khoo: "Constitutively nuclear FOXO3a localization predicts poor survival and promotes Akt phosphorylation in breast cancer." in: PLoS ONE, Vol. 5, Issue 8, pp. e12293, 2010

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN650890) back to top
Supplier Images
Dot Blot (DB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr450) (ABIN650890) Dot blot analysis of anti-Phospho-AKT1-T450 Antibody Phospho-specific Pab (ABIN650890...