anti-Human AKT antibody for Dot Blot

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
pThr450
225
131
115
66
56
51
39
37
36
32
22
22
18
16
13
12
12
12
11
11
10
10
9
9
9
8
7
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1286
670
521
63
58
56
46
35
33
30
18
8
8
6
5
5
3
3
3
2
1
1
1
1
1
Host
Rabbit
925
407
12
4
2
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
54
44
40
38
29
25
18
17
12
12
8
8
7
7
6
6
6
6
6
6
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Dot Blot (DB)
1081
562
484
259
209
175
129
87
66
33
26
15
13
11
11
7
4
4
3
3
3
1
1
1
1
1
1
1
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Catalog No. ABIN650890
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.92041 ABIN650880 DB IF Rabbit Ig pThr308 Log in to see Polyclonal 1
28.92041 ABIN389509 DB Rabbit Ig pSer129 Log in to see Polyclonal 4
28.92041 ABIN389819 DB WB Rabbit Ig pSer473 Log in to see Polyclonal 4
28.92041 ABIN3029462 DB ELISA Rabbit Ig Fraction pSer129 Log in to see Polyclonal
28.92041 ABIN3029463 DB ELISA Rabbit Ig Fraction pThr450 Log in to see Polyclonal
28.92041 ABIN3029455 DB ELISA WB Rabbit Ig Fraction pSer473 Log in to see Polyclonal
28.92041 ABIN3029461 DB ELISA Rabbit Ig Fraction pSer124 Log in to see Polyclonal
28.92041 ABIN3029454 DB IF ELISA Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN5552139 DB EIA Rabbit Ig Fraction pSer473 Log in to see Polyclonal
1 ABIN2615415 DB ELISA IF Rabbit IgG pThr308 Log in to see Polyclonal
1 ABIN1955072 DB ELISA Biotin Rabbit IgG pSer473 Log in to see Polyclonal
1 ABIN1953795 DB ELISA APC Rabbit IgG pSer473 Log in to see Polyclonal
1 ABIN1956237 DB ELISA FITC Rabbit IgG pSer473 Log in to see Polyclonal
1 ABIN1952923 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG pSer473 Log in to see Polyclonal
1 ABIN1953099 DB ELISA ICC IF Alkaline Phosphatase (AP) Rabbit IgG pThr308 Log in to see Polyclonal
1 ABIN1956408 DB ELISA ICC IF FITC Rabbit IgG pThr308 Log in to see Polyclonal
1 ABIN1958744 DB ELISA ICC IF PE Rabbit IgG pThr308 Log in to see Polyclonal
1 ABIN1957448 DB ELISA HRP Rabbit IgG pSer473 Log in to see Polyclonal
1 ABIN1953971 DB ELISA ICC IF APC Rabbit IgG pThr308 Log in to see Polyclonal
1 ABIN1955232 DB ELISA ICC IF Biotin Rabbit IgG pThr308 Log in to see Polyclonal

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pThr450
(225), (131), (115), (66), (56), (51), (39), (37), (36), (32), (22), (22), (18), (16), (13), (12), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1286), (670), (521), (63), (58), (56), (46), (35), (33), (30), (18), (8), (8), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(925), (407), (12), (4), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This AKT antibody is un-conjugated
(54), (44), (40), (38), (29), (25), (18), (17), (12), (12), (8), (8), (7), (7), (6), (6), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Dot Blot (DB)
(1081), (562), (484), (259), (209), (175), (129), (87), (66), (33), (26), (15), (13), (11), (11), (7), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN650890) Images
Specificity This Phospho-AKT1-T450 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide surrounding T450 of human AKT1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This AKT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T450 of human AKT1.
Clone RB29255
Isotype Ig

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN650890) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Synonyms: RAC-alpha serine/threonine-protein kinase,AKT1,PKB, RAC
Molecular Weight 55686 DA
Gene ID 207
NCBI Accession NP_001014431, NP_001014432, NP_005154
UniProt P31749
Research Area Translation Factors, Protein Modifications, Signaling, Neurology, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cancer, Chromatin, Metabolism
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN650890) Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN650890) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-AKT antibody (ABIN650890)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Chen, Gomes, Monteiro, Wong, Wu, Ng, Karadedou, Millour, Ip, Cheung, Sunters, Chan, Lam, Khoo: "Constitutively nuclear FOXO3a localization predicts poor survival and promotes Akt phosphorylation in breast cancer." in: PLoS ONE, Vol. 5, Issue 8, pp. e12293, 2010

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN650890) back to top
Supplier Images
Dot Blot (DB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody (ABIN650890) Dot blot analysis of anti-Phospho-AKT1-T450 Antibody Phospho-specific Pab (ABIN650890...