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|Application / Reactivity||Mouse (Murine)|
|Dot Blot (DB)||1 Antibodies|
|Enzyme Immunoassay (EIA)||26 Antibodies|
|Flow Cytometry (FACS)||23 Antibodies|
|Fluorescence Microscopy (FM)||2 Antibodies|
|Fluorescence Resonance Energy Transfer Microscopy (FRET)||1 Antibodies|
|Immunochromatography (IC)||5 Antibodies|
|Immunocytochemistry (ICC)||22 Antibodies|
|Immunofluorescence (IF)||101 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||32 Antibodies|
|Immunohistochemistry (IHC)||241 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||57 Antibodies|
|Immunoprecipitation (IP)||53 Antibodies|
|Intracellular Staining (ICS)||12 Antibodies|
|Stimulated Emission Depletion Microscopy (STED)||1 Antibodies|
|Western Blotting (WB)||405 Antibodies|
|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This AKT antibody is un-conjugated Alternatives|
Intracellular Staining (ICS), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-AKT AntibodyTarget Details AKT Application Details Handling References for anti-AKT Antibody (ABIN967668) Images
|Cross-Reactivity (Details)||Predicted: Cow, Rat|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. An isotype control should be used at the same concentration as the antibody of interest.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Phosphorylated Human Akt1 (pS473) Peptide|
Target Details AKTProduct Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN967668) Images back to top
|Alternative Name||Akt (AKT1 Antibody Abstract)|
Akt [also known as PKB (Protein kinase B) or RAC-PK (Related to the A and C kinases)] is a family of serine/threonine kinases that contains a pleckstrin homology (PH) domain. PH domains play important roles in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (alpha), Akt2 (beta) and Akt3 (gamma)], they are thought to be regulated similarly. Akt is activated by insulin and growth factors by a mechanism involving phosphoinositide 3-OH kinase. Phosphoinositide 3-OH kinases products bind to the PH domain, resulting in translocation of Akt to the plasma membrane and activation of Akt to phospho-Akt by upstream kinases. Akt is phosphorylated within the activation loop at threonine 308 and the C-terminus at serine 473 (S473). Phospho-Akt promotes cell survival by inhibiting apoptosis. Specifically, phospho-Akt1 has been shown to phosphorylate Bad, a member of the Bcl-2 family that promotes cell death. This phosphorylation results in the inactivation of the proapoptotic function of Bad. The Akt molecule is thus considered to link extracellular survival signals (growth factors) with the apoptotic machinery (BAD). Akt is also a key mediator of the metabolic effects of insulin. Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors. The M89-61 antibody recognizes Akt phosphorylated at S473. This phosphorylation site is shared by all three isoforms of Akt. The homologous phosphorylation sites in Akt2 and Akt3 are S474 and S472, respectively.
Synonyms: Akt1, Akt2, Akt3, PKBalpha, PKBbeta, PKBgamma, RAC-PKalpha, RAC-PKbeta, RAC-PKgamma, STK-2
|Molecular Weight||60 kDa|
|Pathways||PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN967668) Images back to top
Related Products: ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN967668) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-AKT Antibody (ABIN967668)Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
|Product cited in:||
Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, 1999 (PubMed).
Ferrigno, Silver: "Regulated nuclear localization of stress-responsive factors: how the nuclear trafficking of protein kinases and transcription factors contributes to cell survival." in: Oncogene, Vol. 18, Issue 45, pp. 6129-34, 1999 (PubMed).
Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).
Datta, Dudek, Tao et al.: "Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery." in: Cell, Vol. 91, Issue 2, pp. 231-41, 1997 (PubMed).
Alessi, Andjelkovic, Caudwell et al.: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997 (PubMed).
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