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anti-Monkey AKT antibody for ELISA

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
pSer473, Internal Region
185
123
84
65
54
39
31
30
28
22
21
21
18
17
16
14
13
12
12
11
11
10
10
9
9
9
8
7
6
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Monkey
1119
627
487
62
58
55
46
35
33
30
18
8
8
5
5
5
3
3
2
2
1
1
1
1
Host
Mouse
846
317
11
4
1
Clonality
Monoclonal
Conjugate
This AKT antibody is conjugated to Biotin
45
38
35
35
26
25
12
12
7
7
6
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
948
518
433
218
157
148
114
67
54
33
26
15
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN1043752
$ 295.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
1 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Log in to see 18F3-H11 4
1 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 4
1 ABIN968951 ELISA WB Mouse IgG1 Log in to see 3A3 3
1 ABIN964560 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN1099897 IF IHC ELISA WB Biotin Mouse IgG pSer473 Log in to see 17F6-B11
1 ABIN1099900 IHC ELISA WB Biotin Mouse IgG pThr308 Log in to see 18F3-H11
1 ABIN964561 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN964562 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN1099896 ELISA IF IHC (p) WB Mouse IgG1 pSer473 Log in to see 17F6-B11
1 ABIN1099899 ELISA IHC (p) WB Mouse IgG pThr308 Log in to see 18F3-H11
1 ABIN3182568 ELISA IHC WB Rabbit IgG pTyr474 Log in to see Polyclonal
1 ABIN3183233 ELISA IF IHC WB Rabbit IgG Ser13 Log in to see Polyclonal
1 ABIN3181908 ELISA IHC WB Rabbit IgG pThr308 Log in to see Polyclonal
1 ABIN1496551 ELISA WB Mouse IgG1 Log in to see 3A3
1 ABIN965527 ELISA WB Mouse IgG1 Log in to see 3A3 3
1 ABIN4279002 ELISA ICC IF DyLight 488 Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN4279007 ELISA FACS IHC IHC (p) WB Biotin Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN4279006 ELISA FACS ICC IF IHC IHC (p) WB Biotin Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN1099898 IF ELISA WB Atto 594 Mouse IgG1 pSer473 Log in to see 17F6-B11

Top referenced anti-AKT antibody for ELISA

Similar anti-AKT Antibodies

Application / Reactivity Rat (Rattus)
Dot Blot (DB) 1 Antibodies
ELISA 168 Antibodies
Enzyme Immunoassay (EIA) 20 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 49 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 107 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 218 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 90 Antibodies
Immunoprecipitation (IP) 58 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Simple Western (SimWes) 2 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer473, Internal Region
(185), (123), (84), (65), (54), (39), (31), (30), (28), (22), (21), (21), (18), (17), (16), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Monkey
(1119), (627), (487), (62), (58), (55), (46), (35), (33), (30), (18), (8), (8), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(846), (317), (11), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is conjugated to Biotin
(45), (38), (35), (35), (26), (25), (12), (12), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(948), (518), (433), (218), (157), (148), (114), (67), (54), (33), (26), (15), (11), (11), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1043752) Images
Specificity Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
Characteristics AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Immunogen Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Immunogen Type: Peptide
Clone 17F6-B11
Isotype IgG1 kappa

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN1043752) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Background AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Synonyms: phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, phospho AKT antibody, Anti-AKT pS473 Antibody Biotin Conjugated
Gene ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN1043752) Images back to top
Application Notes Biotin Conjugated Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Comment

Gene Name: AKT1

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN1043752) Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-AKT Antibody (ABIN1043752)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1043752) back to top
Supplier Images
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (Biotin) (ABIN1043752) Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST t...
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 2)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 3)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 4)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 5)