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anti-Mouse (Murine) AKT antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Epitope
AA 445-480
187
136
92
88
54
39
38
38
26
25
21
20
18
16
16
15
15
14
13
12
11
11
10
8
7
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1142
531
397
43
41
34
33
32
20
17
10
6
5
4
2
2
2
1
1
1
Host
Rabbit
891
291
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
45
36
35
34
27
25
10
9
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
958
548
433
216
130
120
112
71
57
36
23
12
11
6
3
3
3
3
1
1
1
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN725195
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008846439 ABIN1406506 IF (p) Cy5 Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal
0.0008846439 ABIN1406505 IF (p) Cy3 Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal
0.0008846439 ABIN1406508 IF (p) Cy7 Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal
0.0008846439 ABIN1406507 IF (p) Cy5.5 Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal
0.0008846439 ABIN1388613 IF (p) Alexa Fluor 350 Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal
0.0008846439 ABIN1388614 IF (p) Alexa Fluor 488 Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal
0.0008846439 ABIN1388616 IF (p) Alexa Fluor 647 Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal
0.0008846439 ABIN1388618 IF (p) FITC Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal
0.0008846439 ABIN1388615 IF (p) Alexa Fluor 555 Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal
0.0008846439 ABIN882092 IF (p) Alexa Fluor 488 Rabbit IgG AA 445-480 Log in to see Polyclonal
0.0008846439 ABIN882093 IF (p) Alexa Fluor 350 Rabbit IgG AA 445-480 Log in to see Polyclonal
0.0008846439 ABIN882094 IF (p) Alexa Fluor 555 Rabbit IgG AA 445-480 Log in to see Polyclonal
0.0008846439 ABIN882096 IF (p) Alexa Fluor 647 Rabbit IgG AA 445-480 Log in to see Polyclonal
0.0008846439 ABIN725201 IF (p) Cy7 Rabbit IgG AA 445-480 Log in to see Polyclonal
0.0008846439 ABIN725198 IF (p) Cy3 Rabbit IgG AA 445-480 Log in to see Polyclonal
0.0008846439 ABIN725199 IF (p) Cy5 Rabbit IgG AA 445-480 Log in to see Polyclonal
0.0008846439 ABIN725200 IF (p) Cy5.5 Rabbit IgG AA 445-480 Log in to see Polyclonal
0.0008846439 ABIN725202 IF (p) FITC Rabbit IgG AA 445-480 Log in to see Polyclonal
0.0008846439 ABIN1387775 IF (p) IHC (p) WB Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal
0.0008846439 ABIN2809584 IF (p) Alexa Fluor 594 Rabbit IgG pSer473, pTyr474 Log in to see Polyclonal

Top referenced anti-AKT antibody for Immunofluorescence (Paraffin-embedded Sections)

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Immunofluorescence (IF) 90 Antibodies
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Immunohistochemistry (IHC) 210 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
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Immunoprecipitation (IP) 41 Antibodies
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General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 445-480
(187), (136), (92), (88), (54), (39), (38), (38), (26), (25), (21), (20), (18), (16), (16), (15), (15), (14), (13), (12), (11), (11), (10), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1142), (531), (397), (43), (41), (34), (33), (32), (20), (17), (10), (6), (5), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(891), (291), (14), (4), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(45), (36), (35), (34), (27), (25), (10), (9), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(958), (548), (433), (216), (130), (120), (112), (71), (57), (36), (23), (12), (11), (6), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN725195) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human AKT1
Isotype IgG

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN725195) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Subcellular location: Cytoplasm, Nucleus


Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG
Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN725195) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN725195) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-AKT Antibody (ABIN725195)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, 2014

Xu, Huang, Xie, Lin, Guo, Zhuang, Lin, Zhou, Li, Huang, Wei, Man, Zhang: "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." in: BMC cancer, Vol. 13, pp. 247, 2013

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN725195) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 445-480) (ABIN725195) Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 445-480) (ABIN725195) Mouse placenta lysate with Anti-AKT1/3 Polyclonal Antibody Unconjugated at 1:5000 fo...
'Independent Validation' Badge
Lot Number 131113
Method validated Flow Cytometry
Positive Control MDA-MB-468 cells - lower expression
Notes A strong specific signal is observed in both of the positive control cells stained with anti-AKT1 plus secondary antibody compared with isotype, secondary only and unstained cells.
Primary Antibody
  • Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
  • Catalog number: ABIN725195
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 131113
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Isotype Control Antibody
  • Antibody: Rabbit IgG
  • Catalog number: Log in to see
  • Supplier: Log in to see
  • Lot number: 16
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Secondary Antibody
  • Antibody: Goat anti-rabbit IgG-FITC
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: 115629
  • Dilution: 1:200 in 1X PBS containing 0.5% BSA
Controls
  • Positive control: MCF-7 cells
  • Low positive control: MDA-MB-468 cells
  • Isotype control: Both cell lines treated with rabbit IgG instead of the primary antibody to confirm that primary antibody binding is specific.
  • Secondary only control: Both cell lines treated with Goat anti-rabbit IgG-FITC to confirm no background signal produced from secondary antibody alone
Protocol
  • Control cells were cultured in DMEM + 10% FBS. - Control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
  • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
  • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37 °C.
  • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
  • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA.
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
  • Cells were incubated for 30 min on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
  • Goat anti-rabbit IgG-FITC secondary antibody (Jackson Immunoresearch) was added at a 1:200 dilution. The cells were incubated for 30 min in the dark on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a blue laser (488 nm excitation / 525 nm emission).
Experimental Notes Nothing to note.
Validation Images
Flow Cytometry validation image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Figure 1: Positive control (high expression) MCF7 cells. The red histogram is unstain...
Flow Cytometry validation image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Figure 2: Positive control (lower expression) MDA-MB-468 cells. The red histogram is ...