anti-Rat (Rattus) AKT antibody for Enzyme Immunoassay

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
AA 460-480, C-Term
188
122
85
65
56
39
32
30
28
22
21
21
18
18
17
14
13
12
12
11
11
10
10
9
9
9
8
7
6
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
1136
636
493
63
57
56
46
35
33
30
18
8
8
5
5
5
3
3
3
2
1
1
1
1
Host
Rabbit
855
323
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
48
38
35
35
26
25
12
12
7
7
6
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
964
527
435
219
159
148
115
67
56
32
26
15
11
11
3
3
3
3
3
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN5556658
$ 446.99
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN265302 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN265304 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN5550837 EIA IF IHC (p) IP WB Mouse IgG1 Ser473 Log in to see 17F6-B11
1 ABIN5551855 EIA WB Sheep IgG Internal Region Log in to see Polyclonal
1 ABIN126858 EIA WB Mouse IgG1 AA 466-480 Log in to see 5C10
1 ABIN126853 EIA WB Mouse IgG1 pSer473 Log in to see 1-10E-007
1 ABIN5556660 EIA WB Rabbit AA 302-311, pThr308 Log in to see Polyclonal
1 ABIN5556659 EIA IF IHC (p) WB Rabbit AA 460-480, C-Term Log in to see Polyclonal
1 ABIN126852 EIA WB Biotin Mouse IgG1 pSer473 Log in to see 1-10E-007
1 ABIN126855 EIA IHC (fro) WB Mouse IgG1 AA 107-122 Log in to see 5G12
1 ABIN126857 EIA WB Biotin Mouse IgG1 Log in to see 5C10
1 ABIN615022 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN302693 EIA IHC (p) WB Rabbit pSer473 Log in to see Polyclonal
1 ABIN126854 EIA WB Mouse IgG1 C-Term, Ser473 Log in to see 11A11
1 ABIN615269 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN5554530 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN1105273 EIA WB Rabbit Internal Region, pSer473 Log in to see Polyclonal
1 ABIN5539956 EIA IHC (p) WB Rabbit AA 270-320 Log in to see Polyclonal
1 ABIN2715844 DB EIA ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-AKT Antibodies

Application / Reactivity Rat (Rattus)
Dot Blot (DB) 1 Antibodies
ELISA 171 Antibodies
Enzyme Immunoassay (EIA) 20 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 49 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 107 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 219 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 91 Antibodies
Immunoprecipitation (IP) 58 Antibodies
Intracellular Staining (ICS) 3 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 460-480, C-Term
(188), (122), (85), (65), (56), (39), (32), (30), (28), (22), (21), (21), (18), (18), (17), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Human, Mouse (Murine), Rat (Rattus)
(1136), (636), (493), (63), (57), (56), (46), (35), (33), (30), (18), (8), (8), (5), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(855), (323), (11), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(48), (38), (35), (35), (26), (25), (12), (12), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(964), (527), (435), (219), (159), (148), (115), (67), (56), (32), (26), (15), (11), (11), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Sequence C-R-PH-F-P-Q-F-S-Y-S-A-S-G-T-A
Specificity This antiserum reacts with the AKT.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Mouse and Chicken.
Species reactivity (tested):Human.
Characteristics Concentration: 85 mg/mL (by refractometry)
Purification Serum
Immunogen Synthetic peptide corresponding to the C-terminus (460-480) of Human, Rat and Mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Research Area Kinases/Phosphatases, Protein Modifications, Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes ELISA: 1/2,000-1/10,000. Immunoprecipitation. Transfected/Primary cell culture. Western Blot (1/500-1/2,000): Detect a single band of the expected apparent molecularweight. Immunofluorescence using paraformaldehyde-fixed primary cardiomyocyte cultures(1/100-1/1,000). Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections (1/500-1/2,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Liquid
Concentration 85 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.