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anti-Rat (Rattus) AKT antibody for Flow Cytometry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
AA 2-480
192
136
90
86
55
40
38
37
27
25
21
21
21
20
16
15
15
14
13
12
11
11
10
9
7
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1182
551
417
44
42
41
32
27
20
18
18
6
5
4
2
2
1
1
1
Host
Mouse
910
316
14
4
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
46
39
35
35
26
25
10
10
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Simple Western (SimWes), Western Blotting (WB)
976
555
478
225
142
134
119
71
62
36
26
11
11
5
3
3
3
3
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4899444
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.103481 ABIN4900619 FACS IHC SimWes WB Rabbit IgG pSer473 Log in to see Polyclonal 12
13.103481 ABIN4278999 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
13.103481 ABIN4896123 FACS Allophycocyanin Mouse IgG2b AA 2-480 Log in to see 281046
13.103481 ABIN4896124 FACS PE Mouse IgG2b AA 2-480 Log in to see 281046
13.103481 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6
1 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
1 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
1 ABIN2455513 FACS IF IHC WB Mouse IgG1 Log in to see 4C11
1 ABIN2455515 FACS IF IHC WB Mouse IgG1 Log in to see 1G5
1 ABIN2455510 FACS IF IHC WB Mouse IgG1 Log in to see 4G4
1 ABIN2455508 FACS IF IHC WB Mouse IgG1 Log in to see 4D6
1 ABIN2455509 FACS IF IHC WB Mouse IgG1 Log in to see 3E3
1 ABIN2455511 FACS IF IHC WB Mouse IgG1 Log in to see 3B4
1 ABIN4279007 ELISA FACS IHC IHC (p) WB Biotin Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN4279006 ELISA FACS ICC IF IHC IHC (p) WB Biotin Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN4279000 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN4880525 ELISA FACS ICC WB Mouse IgG1 AA 1-150 Log in to see 1F7E10
1 ABIN4880526 ELISA FACS IHC WB Mouse IgG1 AA 1-150 Log in to see 1F7G2
1 ABIN4278984 FACS Alexa Fluor 647 Mouse IgG2b AA 2-480 Log in to see 281046
1 ABIN4278985 FACS Alexa Fluor 594 Mouse IgG2b AA 2-480 Log in to see 281046

Top referenced anti-AKT antibody for Flow Cytometry

Similar anti-AKT Antibodies

Application / Reactivity Rat (Rattus)
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ELISA 157 Antibodies
Enzyme Immunoassay (EIA) 21 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 33 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 100 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 221 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunoprecipitation (IP) 47 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Simple Western (SimWes) 2 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 2-480
(192), (136), (90), (86), (55), (40), (38), (37), (27), (25), (21), (21), (21), (20), (16), (15), (15), (14), (13), (12), (11), (11), (10), (9), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1182), (551), (417), (44), (42), (41), (32), (27), (20), (18), (18), (6), (5), (4), (2), (2), (1), (1), (1)
Host Mouse
(910), (316), (14), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(46), (39), (35), (35), (26), (25), (10), (10), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Simple Western (SimWes), Western Blotting (WB)
(976), (555), (478), (225), (142), (134), (119), (71), (62), (36), (26), (11), (11), (5), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN4899444) Images
Specificity Detects human, mouse and rat Akt in direct ELISAs and Western blots.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human Akt1, Ser2-Ala480, Accession # P31749
Clone 281046
Isotype IgG2b

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN4899444) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN4899444) Images back to top
Application Notes Western Blot 0.2 μg/mL, Simple Western 2 μg/mL, Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells, Immunocytochemistry 8-25 μg/mL, CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN4899444) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-AKT Antibody (ABIN4899444)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Tolvanen, Dash, Polianskyte-Prause, Dumont, Lehtonen: "Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes." in: Journal of cell science, Vol. 128, Issue 24, pp. 4588-600, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Bifulco, Longanesi-Cattani, Franco, Pavone, Mugione, Di Carluccio, Masucci, Arra, Pirozzi, Stoppelli, Carriero: "Single amino acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion." in: PLoS ONE, Vol. 7, Issue 9, pp. e44806, 2012 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Deng, Han, Yan, Tian, Tao, Kang, Li: "Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt." in: Apoptosis : an international journal on programmed cell death, Vol. 15, Issue 4, pp. 463-73, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Han, Wu, Deng, Tao, Guo, Tian, Kang, Zhang, Yan: "Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation." in: Journal of molecular and cellular cardiology, Vol. 48, Issue 6, pp. 1225-35, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN4899444) back to top
Supplier Images
Simple Western (SimWes) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Human Akt by Simple WesternTM. Simple Western lane view shows lysates of...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Human/Mouse Akt by Western Blot. Western blot shows lysates of MCF-7 hum...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Human Akt Pan Specific by Western Blot. Western blot shows Recombinant H...
Flow Cytometry (FACS) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Detection of Akt in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cance...
Immunocytochemistry (ICC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Akt in MDA-MB-231 Human Cell Line. Akt was detected in immersion fixed MDA-MB-231 hum...
Immunocytochemistry (ICC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 2-480) (ABIN4899444) Akt (pan) in 293T Human Cell Line. Akt pan specific (panels A and C) and Akt1 (E17K M...